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A tomato and gene technology, applied in the field of genetic engineering, can solve problems such as OASTL
Active Publication Date: 2021-07-02
SHANXI UNIV
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[0007] However, there are no reports about OASTL's involvement in plant leaf-type dev
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[0021] Example 1: Tomato Sloas7 Cloning of gene coding regions
[0022] The leaf cDNA from tomato Ailsa Craig is cloned from tomato Ailsa Craig, using nucleotide sequence, such as the forward primer and nucleotide sequence shown in SEQ ID NO. Sloas7 Gene's coding region sequence;
[0029] The above PCR product was directly connected to the PEASY-T1 Simple in accordance with the TA clone method (eg figure 1 The ligated product is transformed into E. coli TOP10, and is expanded therein, and the positive clone is sequencing the sequence to obtain the sequence; its nucleotide sequence such as SEQ ID NO.1, the amino acid sequence encoded by the protein thereof su...
Example Embodiment
[0030] Example 2: Sequence Structure Analysis of Tomato Sloas7 Protein
[0031] Tomato Sloas7 has an OASTL family protein characteristic sequence area: PLP binding region (PXXSVKDR), the similarity of the Cys-C1 protein sequence in the same Arabidopsis is 84%, and the similarity of the Sloas8 protein sequence in tomatoes is 91%. Such as figure 2 Indicated.
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[0032] Example 3: System Evolution Analysis of Tomato Sloas7 Protein
[0033] The system evolution analysis showed that Sloas7 belongs to a Cysc subfamily, and the Cys-C1 in Baneman is a branch in the evolution tree. There is also a Sloas8 in the subfamily in tomatoes. Such as image 3 Indicated.
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Abstract
The invention belongs to the technical field of genetic engineering and provides a tomato S10AS7 gene, the nucleotide sequence of which is shown in SEQ ID No.1. The S10AS7 protein encoded by the gene is composed of the amino acid sequence shown in SEQ ID No.2; or the amino acid sequence shown in SEQ ID No.2 is substituted, deleted and/or increased by one or more amino acids and has the same active derivatized protein. tomato SlOAS7 Gene belongs to the family of genes encoding tomato O‑acetyl‑L‑serine (thiol) lyase (OASTL) and represses in tomato SlOAS7 The expression of the gene can significantly affect the leaf shape and size of tomato plant leaves, resulting in compound leaves with smaller leaf size and reduced complexity. Since the development of plant leaves is closely related to the photosynthetic efficiency, it can be used to improve the photosynthetic efficiency of tomato plants and has good potential application value.
Description
technical field [0001] The invention belongs to the technical field of genetic engineering, in particular to tomato SlOAS7 A gene and its application, the gene belongs to the genes related to leaf development and its application in the process of compound leaf development and leaf size formation. Background technique [0002] Plant leaves are important vegetative organs of higher plants, the main body of plants for photosynthesis, and also important organs for plants to perceive changes in the external environment and receive and transmit environmental signals. In flowering plants, the leaves have various shapes, but according to the shape of the mature leaves, the leaves can basically be divided into two types: simple leaves and compound leaves. [0003] Tomato is an important fruit vegetable and economic crop widely grown all over the world. Tomato leaves are pinnate compound leaves, which are important vegetative organs for photosynthesis of tomato plants. [0004] Fro...
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