Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Tomato sloas7 gene and its application

A tomato and gene technology, applied in the field of genetic engineering, can solve problems such as OASTL

Active Publication Date: 2021-07-02
SHANXI UNIV
View PDF9 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, there are no reports about OASTL's involvement in plant leaf-type development, and detailed reports about OASTL's functions other than catalytic functions.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Tomato sloas7 gene and its application
  • Tomato sloas7 gene and its application
  • Tomato sloas7 gene and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: tomato SlOAS7 Cloning of coding regions of genes

[0022] Utilize the forward primer of nucleotide sequence as shown in SEQ ID No.3 and the reverse primer of nucleotide sequence as shown in SEQ ID No.4, clone from the leaf cDNA of tomato Ailsa Craig SlOAS7 The sequence of the coding region of the gene;

[0023]PCR program: 94°C, 5 minutes; 94°C, 30 seconds; 55°C, 30 seconds; 72°C, 45 seconds; repeat 35 times; 72°C, 10 minutes.

[0024] PCR system: 2× EasyTaq PCR SuperMix (Full Type Gold Company) 25μl;

[0025] Forward primer (10μM) 2μl;

[0026] Reverse primer (10μM) 2μl;

[0027] DNA template 5μl;

[0028] Double distilled water to make up 50μl.

[0029] The above PCR product was directly cloned and connected to pEASY-T1 Simple according to the TA cloning method (such as figure 1 shown); the ligation product was transformed into Escherichia coli Top10, and multiplied in it, and the positive clone was sequenced and screened to obtain the sequence; i...

Embodiment 2

[0030] Embodiment 2: Sequence structure analysis of tomato S10AS7 protein

[0031] Tomato SlOAS7 has the characteristic sequence region of OASTL family proteins: PLP binding region (PXXSVKDR), which has 84% ​​similarity with Cys-C1 protein sequence in Arabidopsis and 91% similarity with SlOAS8 protein sequence in tomato. The result is as figure 2 shown.

Embodiment 3

[0032] Embodiment 3: Phylogenetic analysis of tomato S10AS7 protein

[0033] The results of phylogenetic analysis showed that SlOAS7 belonged to the CysC subfamily, and belonged to the same branch of the phylogenetic tree as Cys-C1 in Arabidopsis. Also belonging to this subfamily in tomato is SlOAS8. The result is as image 3 shown.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of genetic engineering and provides a tomato S10AS7 gene, the nucleotide sequence of which is shown in SEQ ID No.1. The S10AS7 protein encoded by the gene is composed of the amino acid sequence shown in SEQ ID No.2; or the amino acid sequence shown in SEQ ID No.2 is substituted, deleted and / or increased by one or more amino acids and has the same active derivatized protein. tomato SlOAS7 Gene belongs to the family of genes encoding tomato O‑acetyl‑L‑serine (thiol) lyase (OASTL) and represses in tomato SlOAS7 The expression of the gene can significantly affect the leaf shape and size of tomato plant leaves, resulting in compound leaves with smaller leaf size and reduced complexity. Since the development of plant leaves is closely related to the photosynthetic efficiency, it can be used to improve the photosynthetic efficiency of tomato plants and has good potential application value.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, in particular to tomato SlOAS7 A gene and its application, the gene belongs to the genes related to leaf development and its application in the process of compound leaf development and leaf size formation. Background technique [0002] Plant leaves are important vegetative organs of higher plants, the main body of plants for photosynthesis, and also important organs for plants to perceive changes in the external environment and receive and transmit environmental signals. In flowering plants, the leaves have various shapes, but according to the shape of the mature leaves, the leaves can basically be divided into two types: simple leaves and compound leaves. [0003] Tomato is an important fruit vegetable and economic crop widely grown all over the world. Tomato leaves are pinnate compound leaves, which are important vegetative organs for photosynthesis of tomato plants. [0004] Fro...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/60C12N9/88C12N15/82A01H5/12A01H6/82
CPCC12N9/88C12N15/8218C12N15/8261C12Y402/99
Inventor 刘旦梅裴雁曦张彦洁
Owner SHANXI UNIV
Features
  • Generate Ideas
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com