Lactuca sativa extract capable of inhibiting synthesis of melanin and integrated cultivation method of lactuca sativa
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A cultivation method and extraction method technology, applied in the field of plant physiology, can solve the problems of easy discoloration, skin irritation, light sensitivity, etc., and achieve the effect of easy promotion
Active Publication Date: 2020-07-10
INST OF URBAN AGRI CHINESE ACADEMY OF AGRI SCI +1
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[0004]
At present, the main whitening ingredients that inhibit melanin synthesis in the market include arbutin, kojic acid derivatives, ascorbic acid derivatives, hydroquinone derivatives, etc. These whitening agents have significant whitening effects, but there are also skin irritation, photosensitivity, cell Problems such as toxicity and easy discoloration
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Embodiment 1
[0034] 1. Lettuce cultivation
[0035] A, seedling cultivation, select the lettuce (Lactuca sativa L.) seed of kind " Lollo Rossa ", cultivate 10 days in the seedling cultivation plug tray, obtain lettuce seedling;
[0036] b. Cultivation, planting the lettuce seedlings on the floating plate floating on the culture solution in the greenhouse, the cultivation continued for 30 days, and the lettuce was cultivated for 30 days. During the cultivation period, the culture solution grouping was respectively set to the S13 group containing 4.5mmol / L glycine , S15 group containing 9mmol / L glycine, S17 group containing 18mmol / L glycine, the temperature control during the cultivation period is: the average temperature during the day is 22°C, the average temperature at night is 16°C, and the lettuce is harvested;
[0037] c. The whole plant of lettuce is quick-frozen with liquid nitrogen, and then stored in a -80°C refrigerator.
[0038] 2. Extract preparation
[0039] Step 1), take 0.2...
Embodiment 2
[0043] Extract effect monitoring test:
[0044] 1. Determination of cytotoxicity of lettuce extract to B16 mouse melanoma cell line
[0045] Step 1), resuspend the B16 mouse melanoma cells in the culture medium and spread them on a 96-well plate, with about 5000 cells per well, at 37°C, 5% CO 2 Discard the solution after culturing for 24 hours;
[0046] Step 2), dilute the three above-mentioned lettuce extracts with a medium (DMEM medium) containing various amino acids and glucose, that is, 10 μL lettuce extract (S13 group, S15 group, S17 group) and 90 μL DMEM medium Mixed and added to the cells; B16 mouse melanoma cells without lettuce extract were used as a control; for the cell samples added with lettuce extract and the control cell samples, 5 replicate holes were set for each sample;
[0047] Step 3), after 48 hours, detect the inhibitory efficiency of the lettuce extract on cell growth.
[0048] The results showed that compared with the control group, the lettuce extra...
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Abstract
The invention discloses a cultivation method of lactuca sativa. The cultivation method of the lactuca sativa comprises the steps of selecting lactuca sativa seeds of a Lollo Rossa variety, conductingculture in a seedling-growing plug for 10 days, planting obtained lactuca sativa seedlings in floating boards in a greenhouse, and then conducting cryopreservation after 30 days of cultivation. A production method of an extract, with a melanin inhibiting effect, of the lactuca sativas is further disclosed. The production method of the extract, with the melanin inhibiting effect, of the lactuca sativas comprises the steps of subjecting fresh lactuca sativa samples to grinding, extraction, centrifugation and vacuum freeze-drying, and dissolving an obtained product in sterile ultrapure water containing 0.1% dimethyl sulfoxide (DMSO) at last. Furthermore, application of the extract in production of spot-removing make-up, medicines, health-care products, daily chemical products or functional raw materials is disclosed. The provided lactuca sativa extract comes from the natural plants, does not have cytotoxicity, significantly inhibits synthesis of the melanin at the same time, and has wideapplication.
Description
technical field [0001] The invention relates to the field of plant physiology, in particular to a lettuce supporting cultivation method and the preparation and application of a lettuce extract capable of inhibiting melanin synthesis. Background technique [0002] The depth of skin color is mainly determined by the content and distribution of melanin in the skin. Melanin can reduce the damage of ultraviolet rays to the skin. However, when excessive melanin is abnormally accumulated and over-sedated, it will cause age spots, melasma, freckles and other diseases that seriously affect the appearance of the skin. Therefore, the research and development of anti-freckle cosmetics with whitening effect and their functional raw materials are becoming more and more active. [0003] Melanin mainly includes two high-polymer quinones, eumelanin and pheomelanin. The biosynthesis steps of melanin mainly include: tyrosinase catalyzes tyrosine to form dopaquinone; dopaquinone is oxidized t...
Claims
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