Volvariella volvacea culture medium, preparation method and culture method thereof
A medium and straw mushroom technology, applied in the fields of botanical equipment and methods, mushroom cultivation, cultivation, etc., can solve the problems of slow growth speed, low density, low accuracy, etc., and achieve fast growth speed, high density, and mycelium. stout effect
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Embodiment 1
[0095] The straw mushroom medium provided in this example contains 200 parts by weight of potatoes, 20 parts of glucose, 5 parts of peptone, 10 parts of agar, and 25 parts of soybean meal water extract.
[0096] Wherein, the aqueous extract solution of soybean meal is prepared by the following method:
[0097] Boil 25g of soybean meal with 500mL of water for 20 minutes, filter, and take the filtrate to obtain the aqueous extract solution of soybean meal, about 300mL, and set aside.
[0098]Taking the preparation of 1000ml straw mushroom culture medium as an example, the preparation method of straw mushroom culture medium is described as follows:
[0099] Weigh 200g of potatoes, cut into small pieces about 3cm long, add 500ml of water, boil for 15-20min; then use gauze to filter, take the filtrate, which is the potato water extract solution. Take 300ml of potato water extract solution, add 20g of glucose, 5g of peptone, 25g of soybean meal water extract solution and 10g of aga...
Embodiment 2
[0102] The straw mushroom culture medium that present embodiment provides, by weight, it contains 200 parts of potatoes, 20 parts of glucose, 5 parts of peptone, 10 parts of agar, 25 parts of soybean meal water extract, and mass volume ratio (g:ml) is 0.5% calcium sulfate.
[0103] Wherein, taking the straw mushroom culture medium of preparing 1000ml as an example, the preparation method of the straw mushroom culture medium is described as follows:
[0104] Get potato water extract solution (its preparation method is the same as Example 1) 300ml, 20g glucose, 5g peptone, 25g soybean meal water extract, appropriate amount of calcium sulfate dihydrate and 10g agar join in the filtrate, add water to make up to 1000mL, pH Naturally, autoclave, 121°C, 20min. Wherein, the added amount of calcium sulfate dihydrate can ensure that the final concentration of calcium sulfate in the 1000ml straw mushroom culture medium is 0.5%.
Embodiment 3
[0106] The composition and preparation method of the straw mushroom culture medium provided in this example are the same as those in Example 2, except that the concentration of calcium sulfate is 1%.
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