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An FITC test paper strip for detecting benzodiazepine, a preparing method thereof and an application method of the test paper strip

A benzodiazepine and test strip technology, applied in the field of FITC test strips and their preparation, can solve the problems of inability to realize rapid detection of large quantities of samples, time-consuming, complicated operation, etc., and achieve short detection time and simple storage. , the effect of simple operation

Inactive Publication Date: 2018-11-30
郑州左安检测科技有限公司
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Problems solved by technology

[0005] The purpose of the present invention is to overcome the existing methods for detecting benzodiazepines, which are complex in operation, time-consuming, and unable to realize the rapid detection of large batches of samples, and provide a method with simple operation, high sensitivity and detection FITC test strips for detecting benzodiazepines with high speed and low cost, preparation method and application method thereof, so as to realize rapid detection and on-site monitoring of large quantities of benzodiazepine samples

Method used

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  • An FITC test paper strip for detecting benzodiazepine, a preparing method thereof and an application method of the test paper strip
  • An FITC test paper strip for detecting benzodiazepine, a preparing method thereof and an application method of the test paper strip
  • An FITC test paper strip for detecting benzodiazepine, a preparing method thereof and an application method of the test paper strip

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preparation example Construction

[0038] The preparation method of the FITC test strip for detecting benzodiazepine mainly comprises the following steps:

[0039] 1) Label the benzodiazepine antibody with FITC and spray it on the conjugate release pad to prepare the conjugate release pad sprayed with FITC-labeled benzodiazepine antibody;

[0040] 2) Spray the hapten-carrier protein conjugate and goat anti-mouse secondary antibody on the reaction membrane as the test line (T) and quality control line (C) to prepare the hapten-carrier coated with benzodiazepine The detection line of protein conjugates and the reaction membrane of the quality control line coated with goat anti-mouse secondary antibody;

[0041] 3) Assemble the conjugate release pad prepared in 1) and 2), the reaction membrane, the sample absorption pad, the water absorption pad and the bottom plate into a test strip.

[0042] The following step-by-step detailed description:

[0043] 1. Preparation of FITC markers:

[0044] Preparation of FITC-...

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Abstract

An FITC test paper strip for detecting benzodiazepine, a preparing method thereof and an application method of the test paper strip are disclosed. The test paper strip includes a sample absorption pad, a conjugate releasing pad, a reaction film, a water absorbing pad and a bottom plate in order. The conjugate releasing pad is sprayed with an FITC labeled antibody. The reaction film is coated witha hapten-carrier protein conjugate detection line and a goat anti-mouse secondary antibody quality control line. The FITC labeled antibody is utilized to achieve benzodiazepine rapid immunoassay through immunochromatography. The invention also provides the application method for detecting benzodiazepine in a sample by utilizing the test paper strip. The test paper strip has advantages of high sensitivity, capability of accurate quantification, rapid detection, convenient operation, and capability of being practical and economical, and can achieve rapid detection and on-site detection for large-batch benzodiazepine samples.

Description

technical field [0001] The invention relates to the detection of benzodiazepines, in particular to a FITC test strip for quantitatively detecting benzodiazepines by utilizing FITC immunochromatography technology, a preparation method and an application method thereof. Background technique [0002] Benzodiazepines, or amphetamines, are the prototype of a series of synthetic drugs with pronounced stimulant effects on the central nervous system. It is classified as a drug because of the addictive nature of intravenous injection. The adverse reactions caused by benzodiazepines include symptoms such as excessive excitement, restlessness, insomnia, tremor, tension and irritability. Physical tolerance to benzodiazepines develops very quickly, so long-term users must take more and more. The most severe consequence of high doses of benzodiazepines is a toxic neuropathy with symptoms resembling paranoid schizophrenia. Abuse of benzodiazepines often occurs together with abuse of bar...

Claims

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Application Information

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IPC IPC(8): G01N33/94G01N33/68G01N33/577G01N33/558G01N33/533G01N21/64
CPCG01N21/6428G01N33/533G01N33/558G01N33/577G01N33/68G01N33/946
Inventor 贾嘉张振兴吕小翠赵琳周亮崔浩哲张鹏高翔
Owner 郑州左安检测科技有限公司
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