FITC test paper strip for detecting amphetamine as well as preparation method and application method thereof

A technology of test strips and amphetamine, applied in the field of FITC test strips and its preparation, can solve the problems of inability to realize rapid detection of large batches of samples, complicated operation, and long time consumption, and achieve short detection time, simple operation, and simple storage Effect

Inactive Publication Date: 2018-11-13
郑州左安检测科技有限公司
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  • Abstract
  • Description
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Problems solved by technology

[0005] The purpose of the present invention is to overcome the complex operation and time-consuming characteristics of the existing methods for detecting amphetamine, and can not realize the characteristics of rapid detection of large batches of samples, and provide a method with simple operation, high sensitivity, fast detection speed, Low-cost test strip for detecting amphetamine and its preparation method and application method, so as to realize rapid detection and on-site monitoring of large quantities of amphetamine samples

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  • FITC test paper strip for detecting amphetamine as well as preparation method and application method thereof
  • FITC test paper strip for detecting amphetamine as well as preparation method and application method thereof
  • FITC test paper strip for detecting amphetamine as well as preparation method and application method thereof

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preparation example Construction

[0038] The preparation method of the test strip for detecting amphetamine mainly comprises the following steps:

[0039] 1) Label the amphetamine antibody with FITC and spray it on the conjugate release pad to prepare the conjugate release pad sprayed with FITC-labeled amphetamine antibody;

[0040] 2) Spray the hapten-carrier protein conjugate and goat anti-mouse secondary antibody on the reaction membrane as the test line (T) and quality control line (C) to prepare the amphetamine-coated hapten-carrier protein conjugate The detection line of the substance and the reaction membrane of the quality control line coated with goat anti-mouse secondary antibody;

[0041] 3) Assemble the conjugate release pad prepared in 1) and 2), the reaction membrane, the sample absorption pad, the water absorption pad and the bottom plate into a test strip.

[0042] The following step-by-step detailed description:

[0043] 1. Preparation of FITC markers:

[0044] Preparation of FITC-labeled a...

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Abstract

The invention discloses an FITC test paper strip for detecting amphetamine as well as a preparation method and an application method thereof. The test paper strip comprises a sample absorption pad, aconjugate release pad, a reaction membrane, a water absorbing pad and a substrate in sequence, wherein the conjugate release pad is coated with an antibody marked by FITC; the reaction membrane is coated with a detection line of hapten-carrier protein conjugate and a quality control line of a goat-anti-mouse second antibody. Due to adoption of the FITC marked antibody and an immunochromatography technique, rapid immunoassay of amphetamine can be achieved. The invention further provides a method for detecting amphetamine in a sample by using the test paper strip. The test paper strip has the advantages of being high in sensitivity, accurate in quantification, rapid in detection, convenient to operate, economical, and practical, and rapid detection and on-site detection on large scales of amphetamine samples can be achieved.

Description

technical field [0001] The invention relates to the detection of amphetamine, in particular to a FITC test strip for quantitatively detecting amphetamine by using an immunochromatography technique, a preparation method and an application method thereof. Background technique [0002] Amphetamine, or amphetamine, is the prototype of a series of synthetic drugs with pronounced stimulant effects on the central nervous system. It is classified as a drug because of the addictive nature of intravenous injection. Adverse reactions caused by amphetamine include symptoms such as excessive excitement, restlessness, insomnia, tremor, tension and irritability. Physical tolerance to amphetamines develops very quickly, so long-term users must take more and more. The most severe consequence of high doses of amphetamines is a toxic neurosis with symptoms resembling paranoid schizophrenia. Abuse of amphetamines often occurs together with abuse of barbiturates and alcohol. Excessive or rep...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558
CPCG01N33/558
Inventor 贾嘉张振兴吕小翠赵琳周亮崔浩哲张鹏高翔
Owner 郑州左安检测科技有限公司
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