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pH responding type polyoxazoline-nano silver layer by layer self-assembly multilayer film and preparation method thereof

A layer-by-layer self-assembly and polyoxazoline technology, applied in coatings, pharmaceutical formulations, medical science, etc., can solve the problem of difficult removal of bacterial biofilms, achieve broad-spectrum high-efficiency antibacterial properties, easy end groups, and cytotoxicity low effect

Active Publication Date: 2018-12-07
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the problem that the existing obstinate bacterial biofilm is difficult to remove, and provide a layer-by-layer self-assembled multilayer film of pH-responsive polyoxazoline-nanometer silver and its preparation method. The water surface resists bacterial adhesion, and realizes the release of antibacterial agent nano-silver under the stimulation of slightly acidic pH to kill bacteria, realizing the integration of anti-killing

Method used

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  • pH responding type polyoxazoline-nano silver layer by layer self-assembly multilayer film and preparation method thereof
  • pH responding type polyoxazoline-nano silver layer by layer self-assembly multilayer film and preparation method thereof
  • pH responding type polyoxazoline-nano silver layer by layer self-assembly multilayer film and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0037] 1) Prepare the following solutions for use: PBS 0.01M, BPEI 3mg / mL, CSS-AgNPs 1mg / mL, POx 1mg / mL.

[0038] 2) Soak the slides in BPEI solution for pre-assembly, take them out after 30 min, wash them with PBS buffer and dry them three times.

[0039] 3) Soak the slides in the CSS-AgNPs solution, take them out after 30 min, rinse them with PBS buffer and dry them three times.

[0040] 4) Soak the slides in the POx solution, take them out after 30 min, wash them repeatedly with PBS buffer solution and dry them three times.

[0041] 5) Repeat alternate soaking and assembly until the required number of assembly layers is reached.

Embodiment 2

[0043] 1) Prepare the following solutions for use: PBS 0.01M, BPEI 2mg / mL, CSS-AgNPs 2mg / mL, POx 2mg / mL.

[0044] 2) Soak the slides in BPEI solution for pre-assembly, take them out after 30 min, wash them with PBS buffer and dry them three times.

[0045] 3) Soak the slides in the CSS-AgNPs solution, take them out after 30 min, rinse them with PBS buffer and dry them three times.

[0046] 4) Soak the slides in the POx solution, take them out after 30 min, wash them repeatedly with PBS buffer solution and dry them three times.

[0047]5) Repeat alternate soaking and assembly until the required number of assembly layers is reached.

Embodiment 3

[0049] 1) Prepare the following solutions for use: PBS 0.01M, BPEI 2mg / mL, CSS-AgNPs 1mg / mL, POx 1mg / mL.

[0050] 2) Soak the slides in BPEI solution for pre-assembly, take them out after 30 min, wash them with PBS buffer and dry them three times.

[0051] 3) Soak the slides in the CSS-AgNPs solution, take them out after 30 min, rinse them with PBS buffer and dry them three times.

[0052] 4) Soak the slides in the POx solution, take them out after 30 min, wash them repeatedly with PBS buffer solution and dry them three times.

[0053] 5) Repeat alternate soaking and assembly until the required number of assembly layers is reached.

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Abstract

The invention discloses a pH responding type polyoxazoline-nano silver layer by layer self-assembly multilayer film and a preparation method thereof. The self-assembly film can prevent adhesion, has apH responding property, and can controllably release nano silver (an antibacterial agent). The preparation method comprises following steps: at first preparing a functional monomer 2-(2-methoxycarbonyl)ethyl-2-oxazoline, then preparing anionic polyoxazoline (Pox) with negative charges through copolymerization reactions; at the same time, reducing silver ions by utilizing catechol grafted chitosanto obtain nano silver composite sol (CCS-AgNPs); and alternatively assembling polyoxazoline with negative charges and nano silver with positive charges by a static layer by layer self-assembly methodto obtain the multilayer film with a pH responding property. The provided multilayer film can prevent the adhesion of bacteria, and can release nano silver to kill bacteria under the irritation of weak acids, and the functions of bacterium inhibiting and bacterium killing are integrated.

Description

technical field [0001] The invention belongs to the field of medical antibacterial films, and in particular relates to a pH-responsive polyoxazoline-nanometer silver layer-by-layer self-assembled multilayer film and a preparation method thereof. Background technique [0002] In recent decades, due to the high incidence of cardiovascular and various metabolic diseases, the number of patients undergoing medical device implantation surgery has increased. However, in the process of implanting the device in the body, bacteria are easily adhered to the surface, forming a film of bacterial aggregates, that is, bacterial biofilm. This bacterial infection has become one of the leading causes of implant failure. Because once the biofilm on the surface of the device is formed, it is extremely difficult to kill and remove. Compared with free bacteria, bacteria in the membrane are much more resistant to antibiotics and antibacterial agents, and are difficult to be swallowed by macropha...

Claims

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Application Information

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IPC IPC(8): A61L31/08A61L31/10A61L31/16C08G73/06
CPCA61L31/08A61L31/088A61L31/10A61L31/16A61L2300/104A61L2300/404A61L2400/12A61L2420/02A61L2420/06A61L2420/08C08G73/06C08L79/04
Inventor 王征科鲍晓炯黄晓飞金晓强乔丰慧傅倍佳胡巧玲
Owner ZHEJIANG UNIV
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