Multi-gene polymerization effect analytic breeding method for increasing lambing number of goats

A litter size and multi-gene technology, which is applied in biochemical equipment and methods, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of long time-consuming, poor selection effect of single gene molecular markers, low breeding efficiency, etc.

Active Publication Date: 2018-12-07
SOUTH CHINA AGRI UNIV
View PDF4 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to overcome the defects and deficiencies of the prior art in the genetic selection of litter size traits, to provide a multi-gene aggregation molecular marker that affects the litter size traits of goats and a multi-gene aggreg

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Multi-gene polymerization effect analytic breeding method for increasing lambing number of goats
  • Multi-gene polymerization effect analytic breeding method for increasing lambing number of goats
  • Multi-gene polymerization effect analytic breeding method for increasing lambing number of goats

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0076] Example 1 Establishment of PCR-RFLP detection method for GnRHR gene

[0077] (1) Primer design

[0078] According to the goat GnRHR gene sequence (Gene ID: 100860755 on NCBI), a pair of primers was designed using Primer5.0 software. The sequence is as follows:

[0079] GnRHR:

[0080] SEQ ID NO. 4: Upstream primer PCR-F: 5'-TCTTGAAGCTGTATCAGCCATA-3';

[0081] SEQ ID NO. 5: Downstream primer PCR-R: 5'-GTGTTGAAAATTGTGGAGAGTAGA-3'.

[0082] (2) PCR amplification system and program settings

[0083] PCR reaction system: 10μL system contains 1μL of genomic DNA template, 0.2μL of upstream and downstream primers, 5μL TaqDNA polymerase, supplemented to the final volume with deionized double distilled water.

[0084] PCR reaction program: pre-denaturation at 94°C for 2min, 35 cycles (denaturation at 94°C for 30s, annealing at 56°C for 30s, extension at 72°C for 30s), extension at 72°C for 5min, storage at 4°C.

[0085] The PCR reaction product was detected by 2% agarose gel electrophoresis, a...

Example Embodiment

[0102] Example 2 Establishment of PCR-HRM detection method for FSHR gene

[0103] (1) Primer design of high resolution melting curve (HRM)

[0104] According to the goat FSHR gene sequence (Gene ID: 100861291 on NCBI), a pair of primers was designed using Primer5.0 software. The sequence is as follows:

[0105] FSHR:

[0106] SEQ ID NO.6: Upstream primer PCR-F: 5'-TACCAGCTCCCAACGCAGAC-3';

[0107] SEQ ID NO. 7: Downstream primer PCR-R: 5'-GACAGAGTCGATGGTGGCAT-3'.

[0108] (2) High resolution melting curve (HRM) reaction conditions

[0109] Use 55-65°C temperature gradient PCR to know the optimal annealing temperature of primers to obtain specific PCR products to improve the sensitivity of high-resolution melting.

[0110] PCR reaction system (10μL): 1μL of DNA template, 0.3μL of upstream and downstream primers, Dye 5μL, RNase-free water 3.4μL.

[0111] The PCR reaction program is: pre-denaturation at 94°C for 2 minutes, 40 cycles (denaturation at 94°C for 10 seconds, annealing at 60°C for ...

Example Embodiment

[0129] Example 3 Establishment of PCR-RFLP detection method for BMP6 gene

[0130] (1) Primer design

[0131] According to the goat BMP6 gene sequence (Gene ID: 100860793 on NCBI), a pair of primers was designed using Primer5.0 software. The sequence is as follows:

[0132] BMP6:

[0133] SEQ ID NO. 8: Upstream primer PCR-F: 5'-TGTGCCCTCACCTGCTGTCTC-3';

[0134] SEQ ID NO. 9: Downstream primer PCR-R: 5'-CCCGGCCTTCCTCTTTAACTC-3'.

[0135] (2) PCR amplification system and program settings

[0136] PCR reaction system: 10μL system contains 1μL of genomic DNA template, 0.2μL of upstream and downstream primers, 5μL TaqDNA polymerase, supplemented to the final volume with deionized double distilled water.

[0137] PCR reaction program: pre-denaturation at 94°C for 2min, 35 cycles (denaturation at 94°C for 30s, annealing at 59°C for 30s, extension at 72°C for 30s), extension at 72°C for 5min, and storage at 4°C.

[0138] The PCR reaction product was detected by 2% agarose gel electrophoresis, and ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the field of molecular biological technology and molecular marker breeding and especially relates to a multi-gene polymerization effect analytic breeding method for increasinglambing number of goats. In the method, GnRHR, FSHR and BMP6 genes are used as the candidate genes that influence the character of the lambing number of goats. In each of the three genes, there is apolymorphic site, so that by performing GnRHR-FSHR-BMP6 multi-gene polymerization effect model analysis to the molecular markers, it is found that AATTCC is an optimum combined genotype. By means of the optimization of combined genotype, the genetic progress on the character of lambing number of Chongqing Black goats is accelerated, and early-stage selection efficiency of the Chongqing Black goatsis increased, so that the method has important significance to cultivation of new species (lines) of high-quality high-reproductive goats.

Description

technical field [0001] The invention belongs to the technical fields of goat molecular biotechnology and molecular markers, and in particular relates to a breeding method for analyzing the effect of multigene aggregation for increasing the litter size of goats. In the present invention, the aggregation effect analysis of the molecular marker and the litter size of goats is applied to the early breeding work of the litter size traits of goats. Background technique [0002] At present, the degree of intensification of the goat industry is not high, most of them are free-range breeding, and the degree of improved breeds is low, the level of productivity is not high, and the economic benefits are not ideal. Therefore, the healthy and sustainable development of the sheep industry is a top priority. Reproductive traits are important economic traits. For breeding stock, fecundity is productivity, and reproductive performance is directly related to the economic effect of the mutton...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 李耀坤杨新月刘德武柳广斌孙宝丽
Owner SOUTH CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap