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Culture method for obtaining high-content glycerol glucoside algae cells

A breeding method, glucose technology, applied to bacteria, adding compounds to stimulate growth, fermentation, etc., can solve the problems that cannot meet the requirements of industrial application, and achieve the effect of reducing the cost of separation and extraction

Active Publication Date: 2018-12-11
青岛中科蓝智生物科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the content in the cells directly affects the extraction cost, the above-mentioned reporting technology still cannot meet the requirements of industrial applications. How to further increase the content of GG in cells has become a key technology for industrial applications.

Method used

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  • Culture method for obtaining high-content glycerol glucoside algae cells
  • Culture method for obtaining high-content glycerol glucoside algae cells
  • Culture method for obtaining high-content glycerol glucoside algae cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0095] A culture method for obtaining spirulina cells with high content of glycerol glucoside, the reactor used in the method is a 10L plate reactor. The specific implementation of the method includes the following steps:

[0096] (1) Obtain the cultured algae cell stage:

[0097] This algae cell is inoculated in the low-salt freshwater medium Zarrouk substratum (see Table 1 and Table 2 for details), and initial inoculation concentration is 0.2g / L, and the temperature program of the freshwater medium of controlling cultivation this algae cell is: constant temperature 25°C, the light program is: continuous light, the light intensity is 500μE·m -2 ·s -2, the light wavelength range is 400-700nm, the ventilation volume is: 0.3VVM, the gas passed through is mixed air containing carbon dioxide, and the carbon dioxide concentration is 5% (v / v).

[0098] The culture time at this stage is 5-7 days.

[0099] (2) GG elevation stage:

[0100] The algae cells are selected from the cel...

Embodiment 2

[0105] A culture method for obtaining spirulina cells with high content of glycerol glucoside, the reactor used in the method is a 10L plate reactor. The specific implementation of the method includes the following steps:

[0106] (1) Obtain the cultured algae cell stage:

[0107] This algae cell is inoculated in the low-salt freshwater medium Zarrouk culture medium (see table 1 and table 2 for details), and initial inoculation concentration is 0.2g / L, and the temperature program of the freshwater medium of controlling cultivation this algae cell is: constant temperature 25°C, the light program is: continuous light, the light intensity is 500μE·m -2 ·s -2 , the light wavelength range is 400-700nm, the ventilation volume is: 0.3VVM, the gas passed through is mixed air containing carbon dioxide, and the carbon dioxide concentration is 5% (v / v).

[0108] The culture time at this stage is 5-7 days.

[0109] (2) GG elevation stage:

[0110] The algae cells are selected from th...

Embodiment 3

[0115] A culture method for obtaining spirulina cells with high content of glycerol glucoside, the reactor used in the method is a 10L plate reactor. The specific implementation of the method includes the following steps:

[0116] (1) Obtain the cultured algae cell stage:

[0117] This algae cell is inoculated in the low-salt freshwater medium Zarrouk culture medium (see table 1 and table 2 for details), and initial inoculation concentration is 0.2g / L, and the temperature program of the freshwater medium of controlling cultivation this algae cell is: constant temperature 25°C, the light program is: continuous light, the light intensity is 500μE·m -2 ·s -2 , the light wavelength range is 400-700nm, the ventilation volume is: 0.3VVM, the gas passed through is mixed air containing carbon dioxide, and the carbon dioxide concentration is 5% (v / v).

[0118] The culture time at this stage is 5-7 days.

[0119] Wherein, the specific formula of the fresh water medium is Zarrouk med...

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Abstract

The invention discloses a culture method for obtaining high-content glycerol glucoside (GG) algae cells. The method comprises the following steps: a phase of obtaining cultured algae cells; a GG improvement phase I: a culture medium in the phase contains a substance capable of changing osmotic pressure of the cells, and the concentration of the substance in the culture medium is that C1 is greaterthan 300mmol / L and less than or equal to 800mmol / L; and / or a GG improvement phase II: a culture medium in the phase contains a substance capable of changing the osmotic pressure of the cells, and theconcentration of the substance in the culture medium is that C2 is greater than 800mmol / L and less than or equal to 1500mmol / L. According to the culture method disclosed by the invention, the high-content GG algae cells can be obtained through gradual regulation and culture, and large-scale industrial production of GG can be realized.

Description

technical field [0001] The invention relates to a cultivation method for obtaining algae cells with high content of glycerol glucoside, belonging to the technical field of microalgae cell cultivation. Background technique [0002] The statements herein merely provide background information related to the present invention and may not necessarily constitute prior art. [0003] Glycerol glucoside (Glucosylglycerol, GG for short) is a class of glycoside compounds formed by linking glycerol molecules and glucose molecules through glycosidic bonds. It was first discovered in traditional Japanese fermented foods, such as sake, and later studies found that it has a variety of important physiological functions, such as skin moisturizing, dental caries prevention, and protein stabilization. [0004] GG is usually produced during the wine brewing process, but the production efficiency is low. There is only about 1 ml of GG compound in every 300 ml of sake, and GG is completely dissol...

Claims

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Application Information

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IPC IPC(8): C12N1/38C12N1/20C12P19/44
CPCC12N1/20C12N1/38C12P19/44
Inventor 吕雪峰段仰凯张凯吴怀之刘祥
Owner 青岛中科蓝智生物科技发展有限公司
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