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A fermented liquid rich in ergonovine and its preparation method

A technology of ergometrine and fermentation liquid, which is applied in the field of microbial fermentation engineering and can solve the problems of fast action and high cost

Active Publication Date: 2021-12-03
NEW FOUNDER HLDG DEV LLC +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Ergometrine is a commonly used drug in obstetrics and gynecology. It is mainly used for postpartum hemostasis and contraction of the uterus. Compared with ergot poisonous alkaloids, it has the characteristics of quick action and low toxicity. Many countries use wild ergot or artificially cultivated ergot as raw materials. Production is often limited by regions and climatic conditions, and mass production cannot be carried out in a short period of time, and large farmland is required, and the cost is relatively high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The present embodiment provides a method of preparing a fermentation broth rich ergometrine, includes the following steps:

[0033] 1) Preparation of a liquid in a liquid fermentation medium in a fermentor, the fermentor as 5000L, 3000L charging amount of the fermentation broth comprising the component and component content: 350Kg glucose, maltodextrin 90Kg, 23Kg yeast, soybean meal powder 150Kg, magnesium 3Kg, remainder water; wherein the initial pH of the liquid fermentation medium was 7.4.

[0034] 2) the liquid fermentation medium is sterilized, the initial pH of the liquid fermentation medium after sterilization was 7.3.

[0035] 3) The bacterial solution aseptically transferred to a liquid tank seed fermentation medium, wherein the volume percentage of 8% inoculation, the fermentation temperature is 28 ~ 32 ℃, the tank pressure is 0.03 ~ 0.06mpa, an aeration amount of 1: 0.8, rotational speed 180 r / min, culture 440h.

[0036] To give a titer of 3325ug / ergometrine ...

Embodiment 2

[0038] The present embodiment provides a method of preparing a fermentation broth rich ergometrine, includes the following steps:

[0039] 1) Preparation of a liquid in a liquid fermentation medium in a fermentor, the fermentor as 5000L, 3000L charging amount of the fermentation broth comprising the component and component content: 250Kg glucose, maltodextrin 90Kg, 15Kg yeast, soybean meal powder 150Kg, magnesium 3Kg, remainder water; wherein the initial pH of the liquid fermentation medium was 7.8;

[0040] 2) the liquid fermentation medium is sterilized, the initial pH of the liquid fermentation medium after sterilization was 7.5.

[0041] 3) The bacterial solution aseptically transferred to a liquid tank seed fermentation medium, wherein the volume percentage of 5% inoculation, fermentation temperature is 28 ~ 32 ℃, the tank pressure is 0.03 ~ 0.06mpa, an aeration amount of 1: 0.8, rotational speed 180 r / min, culture 432h.

[0042] Discharge tank, to give a titer of 3126ug / ...

Embodiment 3

[0044] Fermentation broth provided a rich source ergometrine the method of the present embodiment, includes the following steps:

[0045] 1) Preparation of a liquid in a liquid fermentation medium in a fermentor, the fermentor as 5000L, 3000L charging amount of the fermentation broth comprising the component and component content: glucose 300Kg, 180Kg maltodextrin, 24Kg yeast, soybean meal powder 200Kg, magnesium 4Kg, remainder water; wherein the initial pH of the liquid fermentation medium was 7.6;

[0046] 2) the liquid fermentation medium is sterilized, the initial pH of the liquid fermentation medium after sterilization is 7.2.

[0047] 3) The bacterial solution aseptically transferred to a liquid tank seed fermentation medium, wherein the volume percentage of 6% inoculation, fermentation temperature is 28 ~ 32 ℃, the tank pressure is 0.03 ~ 0.06mpa, an aeration amount of 1: 1, the rotational speed 180 r / min, culture 456h.

[0048] Discharge tank, to give a titer of 3323ug / ...

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PUM

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Abstract

The invention relates to the field of microbial fermentation engineering, in particular to a fermented liquid rich in ergometrine and a preparation method thereof. The preparation method of the present invention is that the seed bacterium liquid of ergot fungus is inserted into the liquid fermentation medium, and the ergonovine fermentation liquid is obtained through shaking table culture; wherein, the liquid fermentation medium comprises: glucose 80-120g / L, maltodextrin 20-60g / L, yeast powder 4-8g / L, soybean meal powder 50-80g / L, magnesium sulfate 0.7-1.5g / L. The preparation method of the ergometrine fermented liquid provided by the present invention has the advantages of low material cost, simple and convenient operation, suitable conditions and stable results, and the fermented liquid prepared by the method provided by the present invention has a ergometrine content of More than 3000ug / ml, it also has the advantages of high liquid chromatography components and no impurities of higher components.

Description

Technical field [0001] The present invention relates to the field of microbial fermentation engineering, in particular, it relates to a process for preparing a fermentation broth rich and ergometrine. Background technique [0002] Ergometrine commonly used drugs in clinical obstetrics and gynecology, and is mainly used for hemostasis post-partum uterine contraction, and the like ergot muscarinic action compared with fast, low toxicity characteristics, many countries in the wild or cultivated ergot ergot as a raw material production, often restricted area and climatic conditions, etc., can not be mass-produced in a short time, and require large tracts of farmland, the cost is higher. Industrial fermentation production using an ergot overcome these difficulties and disadvantages promising approach. Inventive content [0003] The present invention provides a fermentation broth a rich source ergometrine preparation, which can be obtained high titer, high parts group fermentation bro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P17/18C12R1/645
CPCC12P17/183
Inventor 雷川洋杜森林张葵
Owner NEW FOUNDER HLDG DEV LLC