A super-resolution imaging method, device and terminal equipment
A technology of super-resolution imaging and super-resolution images, which is applied in the field of image processing and can solve problems such as functional super-resolution imaging and super-resolution imaging.
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Embodiment 1
[0063] like figure 1 As shown, the embodiment of the present invention provides a super-resolution imaging method, which can be applied to super-resolution positioning imaging of an optical reconstruction microscope, which includes:
[0064] S101. Using the excitation light, excite the fluorescent probe with double emission peaks on the biological sample, and act on the excited fluorescent probe with an affinity reagent.
[0065] In the above step S101, after the fluorescent probe with dual emission peaks is excited, two emission peaks will be displayed in the fluorescence emission spectrum of the fluorescent probe, namely a short-wavelength emission peak and a long-wavelength emission peak.
[0066] In specific applications, before the excitation light acts on the biological sample, it should also pass through optical processing elements, such as lenses, field diaphragms, tube mirrors, objective lenses, etc., so that the excitation light can be uniformly and concentratedly ir...
Embodiment 2
[0094] like figure 2 As shown, the embodiment of the present invention exemplarily shows a detailed implementation process of S102 in the first embodiment above, where step S102 is:
[0095] S102, irradiating the fluorescent probe after the affinity reagent has been acted on by activating light to obtain a dual-channel fluorescence intensity image.
[0096] Its detailed implementation process includes:
[0097] S1021. Obtain the short-wavelength signal according to the first structure in the fluorescent probe.
[0098] S1022. Obtain the long-wavelength signal according to the second structure in the fluorescent probe.
[0099] In the above step S1021 and step S1022, the wavelength of the activation light is the same as the wavelength of the excitation light, wherein the short wavelength emission peak is caused by the first structure in the probe, and the long wavelength emission peak is caused by the second structure in the probe, And the fluorescence emission spectra of t...
Embodiment 3
[0108] like Figure 4 As shown, the embodiment of the present invention exemplarily shows a detailed implementation process of step S103 in the first embodiment above, where step S103 is:
[0109] S103. Separately collect short-wavelength signals of short-wavelength emission peaks and long-wavelength signals of long-wavelength emission peaks in the dual-channel fluorescence intensity image.
[0110] Its detailed implementation process includes:
[0111] S1031. Separate the excitation light from the short-wavelength signal and the long-wavelength signal by using a first dichroic mirror.
[0112] In the above step S1031, the dichroic mirror almost completely transmits light of a certain wavelength, and almost completely reflects light of other wavelengths.
[0113] In a specific application, after the excitation light acts on the biological sample, the light emitted by the fluorescent probe on the biological sample includes a short-wavelength signal, a long-wavelength signal, ...
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