Biocontrol microbial inoculum composition for controlling gray mold and leaf mold of tomatoes

A technology for tomato gray mold and biocontrol fungicides, which is applied in the directions of biocides, fungicides, biocides, etc., can solve the problem of low commercialization rate of biocontrol bacteria resources, large differences in control effects between indoor tests and field tests, and different types of biocontrol bacteria. and limited quantity

Inactive Publication Date: 2018-12-14
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although great progress has been made in the research on biological control of plant diseases including tomato gray mold and leaf mold, there are still many common problems: 1) The resources of biocontrol microorganisms are extremely rich, but most of the researches are only in the laboratory stage , the types and quantities of preparations or products that can be used for production are limited, and the commercialization rate of biocontrol bacteria resources is low, which is difficult to meet production needs; 2) There is a lack of excellent strains with strong adaptability, stable control effect, and wide antibacterial range; 3) The biocontrol mechanism of the strain is not yet clear, and the control effects of laboratory tests and field tests vary greatly
In addition, the use of a single strain for disease control often leads to poor adaptability of biocontrol bacteria and unstable control effects.

Method used

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  • Biocontrol microbial inoculum composition for controlling gray mold and leaf mold of tomatoes
  • Biocontrol microbial inoculum composition for controlling gray mold and leaf mold of tomatoes
  • Biocontrol microbial inoculum composition for controlling gray mold and leaf mold of tomatoes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Preparation of Preliminary Example 1 Biocontrol Bacteria Nitrogenous Pseudomonas WXCDD51 Inoculant

[0038] The WXCDD51 bacterial agent was prepared according to the conventional preparation method of the Pseudomonas azogenes bacterial agent.

Embodiment 2

[0039] Preparation of Preliminary Example 2 Biocontrol Bacillus subtilis (Bacillus sp) WXCDD105 strain bacterial agent

[0040] The WXCDD105 bacterial agent was prepared according to the conventional preparation method of the Bacillus subtilis bacterial agent.

Embodiment 3

[0041] Preparation of Preliminary Example 3 Biocontrol Bacteria Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) Ba strain bacterial agent

[0042] The B strain inoculum was prepared according to the medium formula and fermentation method provided by the China Agricultural Microorganism Culture Collection Management Center.

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Abstract

The invention discloses a biocontrol microbial inoculum composition for controlling gray mold and leaf mold of tomatoes, and belongs to the field of biocontrol of the gray mold and the leaf mold of the tomatoes. The compound biocontrol microbial inoculum composition comprises a bacillus amyloliquefaciens Ba strain and a bacillus sp Bs wy-1 strain, wherein the microbial inoculum composition comprising the bacillus amyloliquefaciens Ba strain and the bacillus sp Bs wy-1 strain according to a mass ratio of 1:3 has the highest rate of inhibiting gray mold bacteria; the microbial inoculum composition obtained by mixing the bacillus amyloliquefaciens Ba strain and the bacillus sp Bs wy-1 strain according to a mass ratio of 3:1 has the highest rate of inhibiting leaf mold bacteria. The biocontrolmicrobial inoculum composition has a significant preventive or therapeutic effect on both the gray mold and the leaf mold of the tomatoes, and the disease preventing effect thereof is significantly better than that of a single strain and that of a combination of other strains.

Description

technical field [0001] The invention relates to a compound biocontrol fungicide composition, in particular to a compound biocontrol fungicide composition for preventing and treating tomato gray mold and leaf mold, and belongs to the field of biological control of tomato gray mold and leaf mold. Background technique [0002] China is a major producer of tomatoes. Since 2013, the annual output of tomatoes in China has been more than 50 million tons, and it has been increasing year by year. The occurrence of diseases often has a serious impact on tomato production, among which botrytis cinerea and leaf mold are two main types of intractable diseases, which cause huge economic losses to tomato production. At present, the two diseases are mainly controlled by chemical pesticides. In recent years, the harm caused by long-term application of pesticides has received great attention. In contrast, biological control has the advantages of no residue and no pollution, and is the first...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01P3/00
CPCA01N63/00
Inventor 王傲雪石玉莹孟令波陈秀玲冉东升刘佳音张瑶张淑梅王迎春康春生姜景彬张贺宫香余
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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