Bacillus amyloliquefaciens and application thereof in degradation of zearalenone
A technology of zearalenone and amylolytic spores, applied in the biological field, can solve the problems of difficult operation, high cost, and difficulty in large-scale use, and achieve good application prospects
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Embodiment 1
[0023] Embodiment 1, the separation and identification of bacteria
[0024] 1. Isolation of bacteria
[0025] (1) In October 2013, in the ultra-clean workbench, the collected Hunan corn samples were shaken in sterile distilled water for 15 minutes to prepare the bacterial suspension, and the shaker speed was 180rpm.
[0026] (2) Dilute the bacterial suspension in a concentration gradient with sterile distilled water, spread it on the NA medium plate, and cultivate it at 30°C for 24 hours. The colonies cover the entire plate. Use an inoculation loop to pick out the shape, size, Strains with different colors and transparency were streaked and purified on plates, and the purified strains were applied to the aflatoxin degradation experiment. A strain with strong aflatoxin degradation ability was obtained and named NS2.
[0027] 2. Identification
[0028] 1. Morphological and physiological and biochemical characteristics
[0029] According to the method described in "Berger Bac...
Embodiment 2
[0036] Embodiment 2, Bacillus amyloliquefaciens NS2 degrades zearalenone
[0037] 1. Culture activation
[0038]Inoculate Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) NS2CGMCC No.8726 in liquid NB medium, shake and cultivate for 24 hours at a temperature of 28°C and a rotation speed of 200rpm (radius of rotation: 20mm), and record all the substances in the culture vessel. As NS2 culture medium, NS2 culture medium is used for subsequent zearalenone degradation experiments.
[0039] 2. Degradation of zearalenone by Bacillus amyloliquefaciens NS2
[0040] 1), the preparation of zearalenone solution:
[0041] The 5mg zearalenone standard substance ( Product No. Z2125) was dissolved in 5 ml of chromatographically pure methanol to obtain a zearalenone solution with a concentration of 1000 ppm.
[0042] 2), degradation experiment
[0043] Experimental group: 5 μl of zearalenone solution with a concentration of 1000 ppm was placed in a 10 ml centrifuge tube, and 995 μ...
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