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Bacillus amyloliquefaciens and application thereof in degradation of zearalenone

A technology of zearalenone and amylolytic spores, applied in the biological field, can solve the problems of difficult operation, high cost, and difficulty in large-scale use, and achieve good application prospects

Inactive Publication Date: 2014-08-13
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among the physical removal methods, methods such as mechanical classification treatment, high temperature inactivation, radiation treatment or extraction of pollutants also have disadvantages such as high cost and difficult operation, making it difficult to use them on a large scale

Method used

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  • Bacillus amyloliquefaciens and application thereof in degradation of zearalenone
  • Bacillus amyloliquefaciens and application thereof in degradation of zearalenone
  • Bacillus amyloliquefaciens and application thereof in degradation of zearalenone

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1, the separation and identification of bacteria

[0024] 1. Isolation of bacteria

[0025] (1) In October 2013, in the ultra-clean workbench, the collected Hunan corn samples were shaken in sterile distilled water for 15 minutes to prepare the bacterial suspension, and the shaker speed was 180rpm.

[0026] (2) Dilute the bacterial suspension in a concentration gradient with sterile distilled water, spread it on the NA medium plate, and cultivate it at 30°C for 24 hours. The colonies cover the entire plate. Use an inoculation loop to pick out the shape, size, Strains with different colors and transparency were streaked and purified on plates, and the purified strains were applied to the aflatoxin degradation experiment. A strain with strong aflatoxin degradation ability was obtained and named NS2.

[0027] 2. Identification

[0028] 1. Morphological and physiological and biochemical characteristics

[0029] According to the method described in "Berger Bac...

Embodiment 2

[0036] Embodiment 2, Bacillus amyloliquefaciens NS2 degrades zearalenone

[0037] 1. Culture activation

[0038]Inoculate Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) NS2CGMCC No.8726 in liquid NB medium, shake and cultivate for 24 hours at a temperature of 28°C and a rotation speed of 200rpm (radius of rotation: 20mm), and record all the substances in the culture vessel. As NS2 culture medium, NS2 culture medium is used for subsequent zearalenone degradation experiments.

[0039] 2. Degradation of zearalenone by Bacillus amyloliquefaciens NS2

[0040] 1), the preparation of zearalenone solution:

[0041] The 5mg zearalenone standard substance ( Product No. Z2125) was dissolved in 5 ml of chromatographically pure methanol to obtain a zearalenone solution with a concentration of 1000 ppm.

[0042] 2), degradation experiment

[0043] Experimental group: 5 μl of zearalenone solution with a concentration of 1000 ppm was placed in a 10 ml centrifuge tube, and 995 μ...

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Abstract

The invention discloses a bacillus amyloliquefaciens and an application thereof in degradation of zearalenone and provides a bacillus amyloliquefaciens NS2 which is assigned the accession number CGMCC No.8726. An experiment in the invention proves that the amyloliquefaciens NS2 is disclosed in the invention. The zearalenone can be degraded efficiently when a fermentation liquor of the amyloliquefaciens NS2 is added to the zearalenone with dissolving and mixing uniformly. The amyloliquefaciens NS2, used as a biological material for degrading the zearalenone, has a good application prospects in exploitation of not only novel biodegradation inoculants but also novel biodegradation sterile preparations.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a strain of bacillus amyloliquefaciens and its application in degrading zearalenone. Background technique [0002] Zearalenone (ZEN) is an estrogen-like toxin produced by Fusarium fungi, which can cause abnormal reproductive functions such as abortion, stillbirth, and return to oestrus in animals, and can also lead to growth decline, immunosuppression, infertility, and deformity. Wait. The pollution of zearalenone in feed and feed raw materials is serious. [0003] Traditional zearalenone detoxification methods include physical and chemical methods, including ammonification method, alkali method, high temperature method, ray irradiation method and ultrafiltration-diafiltration method, etc. These methods have unstable effects and loss of nutrients Large, complex degradation products, difficult to determine the toxicity of degradation products, and difficulty in large-scale productio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L1/015A23K1/16C12R1/07A23K10/16A23L5/20
Inventor 刘阳赵月菊娅娃·米妮·埃尔迪·富丽兰茨纳·桑格邢福国周露王龑
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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