Method for <60>Co-gamma radiation mutation breeding of new tulipa gesneriana variety with protective agent

A protective agent and tulip technology, which is applied in the field of cultivating new varieties of tulips by using radiation mutation breeding technology, can solve the problems that have not yet been seen in the application research of protective agents, and achieve the effects of increasing the mutation rate, expanding the mutant population, and reducing the short growth cycle

Active Publication Date: 2018-12-18
SOUTHWEAT UNIV OF SCI & TECH
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the prior art, there is no related research on the application of protec

Method used

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  • Method for &lt;60&gt;Co-gamma radiation mutation breeding of new tulipa gesneriana variety with protective agent
  • Method for &lt;60&gt;Co-gamma radiation mutation breeding of new tulipa gesneriana variety with protective agent
  • Method for &lt;60&gt;Co-gamma radiation mutation breeding of new tulipa gesneriana variety with protective agent

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Experimental program
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Effect test

Embodiment 1

[0037] Embodiment 1: The effect of protective agent treatment on the survival rate of tulips after gamma ray irradiation:

[0038] This experiment was carried out in the multi-span greenhouse of the School of Life Science and Engineering, Southwest University of Science and Technology. use 60 Co-γ irradiated healthy tulip bulbs, the radiation dose was 5Gy, 10Gy, 20Gy, 40Gy, 60Gy, 80Gy, 100Gy, and the radiation dose was 2.57Gy / min. Two controls were set up in the experiment, one was unirradiated and unwatered bulbs (CK 1 ), the second is the bulbs soaked in deionized water for 30 minutes (CK 2 ). According to 3 different melatonin concentrations for each radiation dose (see Table 1-1 for specific melatonin formulations), soak for 30 minutes respectively, dry and plant. The tulip planting time is January 20th, planted in a 10cm*10cm*30cm black nutrient pot, the soil formula is special soil for plant cultivation: fermented organic substrate: leaf humus: vermiculite = 2:1:1:1 ...

Embodiment 2

[0051] Embodiment 2: The effect of protective agent treatment on tulip vegetative growth after gamma ray irradiation:

[0052] This experiment was carried out in the multi-span greenhouse of the School of Life Science and Engineering, Southwest University of Science and Technology. use 60 Co-γ irradiated healthy tulip bulbs, the radiation dose was 5Gy, 10Gy, 20Gy, 40Gy, 60Gy, 80Gy, 100Gy, and the radiation dose was 2.57Gy / min. Two controls were set up in the experiment, one was unirradiated and unwatered bulbs (CK 1 ), the second is the bulbs soaked in deionized water for 30 minutes (CK 2 ). According to 3 different melatonin concentrations for each radiation dose (see Table 2-1 for the specific melatonin formula), soak for 30 minutes respectively, dry and plant. The tulip planting time is January 20th, planted in a 10cm*10cm*30cm black nutrient pot, the soil formula is special soil for plant cultivation: fermented organic substrate: leaf humus: vermiculite = 2:1:1:1 (mixe...

Embodiment 3

[0062] Embodiment 3: the effect of protective agent treatment on tulip reproductive growth after gamma ray irradiation:

[0063] This experiment was carried out in the multi-span greenhouse of the School of Life Science and Engineering, Southwest University of Science and Technology. use 60 Co-γ irradiated healthy tulip bulbs, the radiation dose was 5Gy, 10Gy, 20Gy, 40Gy, 60Gy, 80Gy, 100Gy, and the radiation dose was 2.57Gy / min. Two controls were set up in the experiment, one was unirradiated and unwatered bulbs (CK 1 ), the second is the bulbs soaked in deionized water for 30 minutes (CK 2 ). According to 3 different melatonin concentrations for each radiation dose (see Table 1-1 for specific melatonin formulations), soak for 30 minutes respectively, dry and plant. The tulip planting time is January 20th, planted in a 10cm*10cm*30cm black nutrient pot, the soil formula is special soil for plant cultivation: fermented organic substrate: leaf humus: vermiculite = 2:1:1:1 (m...

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Abstract

The invention discloses a method for <60>Co-gamma radiation mutation breeding of a new tulipa gesneriana variety with a protective agent. The method is characterized by including: soaking <60>Co-gammaradiated tulipa gesneriana seed balls under a dose rate of 2.57Gy/min in a protective agent aqueous solution, then performing airing and conducting planting. The protective agent is any one of or a mixture of melatonin, gibberellin and salicylic acid. Furthermore, the protective agent aqueous solution can be sprayed to tulipa gesneriana plants in the tulipa gesneriana planting process. Accordingto the invention, soaking treatment of the radiated seed balls with the protective agent can reduce damage to the seed balls after irradiation mutation breeding, increase the germination rate and survival rate of the seed balls, promote the normal growth of the seed balls during the vegetative and reproductive stages, and increase the variation rate. A plant spraying way is adopted for secondary protection, can improve the seedling formation and fertility of tulipa gesneriana, increase the number of flowering plants and increase the variation rate of flowers, thus being beneficial to breedingof a new tulipa gesneriana variety in a short time.

Description

technical field [0001] The invention belongs to a method for obtaining new plants, and relates to a method of using a protective agent in 60 The method for cultivating new varieties of tulips by Co-γ radiation mutation is particularly suitable for cultivating new varieties of tulips by means of radiation mutation breeding technology. Background technique [0002] With the development and wide application of nuclear technology, the opportunities for people to be exposed to radiation are increasing day by day. The application of radioprotectants has attracted much attention. Researchers have been devoting themselves to the study of highly efficient, low-toxic natural radiation protectors. [0003] Since Muller [Muller H J. X-ray mutation generation in 1927 [J]. Proceedings of the National Academy of Sciences of the United States, 1928, 14 (9): 714-726.] discovered the mutagenic effect of radiation on organisms, ionizing radiation mutagenesis technology has been Used in plan...

Claims

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Application Information

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IPC IPC(8): A01H1/06A01C1/00A01G22/35A01G7/06
CPCA01C1/00A01G7/06A01H1/06A01G22/35
Inventor 王丹黎熠睿湛晓蝶刘玲闵可怜
Owner SOUTHWEAT UNIV OF SCI & TECH
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