A kind of Rhododendron tissue culture rapid propagation method

A tissue culture, azalea technology, applied in the directions of horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of difficulty in controlling the time of mercuric chloride treatment, difficulty in flower bud differentiation, difficulty in disinfection and sterilization, etc. The effect of raising seedlings of new varieties, improving the survival rate of hardening and domestication, and improving the rooting rate outside the bottle

Active Publication Date: 2018-10-23
HUAYUAN LANDSCAPE ARCHITECTURE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] (1) The cost of tissue culture and reproduction is high and the technical requirements are also high. How to shorten the time of tissue culture and reproduction to save costs and effectively improve the survival rate of tissue culture seedlings has become the key to tissue culture and reproduction
[0005] (2) The differentiation of flower buds is relatively difficult and the cycle is long; the material of the stem tip is limited by a certain period of time. Generally, stem nodes or leaves are used as explants for primary culture, but there are glandular hairs on the branches and leaves of Rhododendron, and they are very viscous. , will be stained with a lot of dirt and bacteria, it is not easy to disinfect, but if the disinfectant is used too much, it will cause the branches and leaves to wither
[0006] (3) Excessive mercury treatment time will easily cause browning and death of the explant material. If the treatment is too short, the disinfection will not be complete. Controlling the time of mercury treatment becomes a difficult problem.
[0007] (4) It is not easy to control the type and dosage of hormones in the bud culture of Rhododendron

Method used

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  • A kind of Rhododendron tissue culture rapid propagation method
  • A kind of Rhododendron tissue culture rapid propagation method
  • A kind of Rhododendron tissue culture rapid propagation method

Examples

Experimental program
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Effect test

Embodiment 1

[0033] The rhododendron tissue culture rapid propagation method that the present embodiment provides, comprises the following steps:

[0034] (1) Obtain Rhododendron aseptic sowing seedlings, rhododendron seeds are treated with 150mg / L gibberellin for 8 hours and then use 75% (volume percentage content) alcohol (add 1mL sterilized spit in every 100mL alcohol) temperature (80°C) for soaking for 5 minutes, then disinfected with 0.1% (mass percent) mercuric chloride on the ultra-clean workbench for 7 minutes, then sterilized with 10% (mass percent) NaClO for 9 minutes, and finally rinsed with sterile water for 4 minutes ~5 times, use sterilized tweezers to inoculate the sterilized rhododendron seeds on the 1 / 2Read medium without any hormone, and then aseptically cultivate them for about 30 days to obtain sterile seedlings, such as figure 1 shown;

[0035] (2) Obtain a large amount of sterile clustered seedlings, remove the roots of the above-mentioned sterile seedlings and inocu...

Embodiment 2

[0044] The rhododendron tissue culture rapid propagation method that the present embodiment provides, comprises the following steps:

[0045] (1) Acquisition of rhododendron aseptic sowing seedlings: select rhododendron seeds, soak them in alcohol containing Tween 80 after treatment with gibberellin, then sterilize with mercuric chloride and sodium hypochlorite, and rinse with sterile water to obtain sterilized For rhododendron seeds, inoculate sterilized rhododendron seeds on 1 / 2Read medium for aseptic culture to obtain aseptic seedlings of rhododendron;

[0046](2) Clumpy seedling induction: remove the roots of the rhododendron aseptic seedlings obtained in step (1) and inoculate them on the Read medium containing zeatin ZT to induce clustered seedlings to obtain a large number of tissue cultured seedlings;

[0047] (3) Rooting outside the bottle of tissue culture seedlings: select the cultivation medium, cut the stem of the tissue culture seedlings obtained in step (2), cut...

Embodiment 3

[0058] The rhododendron tissue culture rapid propagation method that the present embodiment provides, comprises the following steps:

[0059] (1) Acquisition of rhododendron aseptic sowing seedlings: select rhododendron seeds, soak them in alcohol containing Tween 80 after treatment with gibberellin, then sterilize with mercuric chloride and sodium hypochlorite, and rinse with sterile water to obtain sterilized For rhododendron seeds, inoculate sterilized rhododendron seeds on 1 / 2Read medium for aseptic culture to obtain aseptic seedlings of rhododendron;

[0060] (2) Clumpy seedling induction: remove the roots of the rhododendron aseptic seedlings obtained in step (1) and inoculate them on the Read medium containing zeatin ZT to induce clustered seedlings to obtain a large number of tissue cultured seedlings;

[0061] (3) Rooting outside the bottle of tissue culture seedlings: select the cultivation medium, cut the stem of the tissue culture seedlings obtained in step (2), cu...

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Abstract

The invention discloses a rhododendron tissue culture and rapid propagation method which comprises the following steps: (1) obtaining rhododendron sterile sowing seedlings; (2) performing clustered seedling induction; (3) performing ex-vitro rooting on tissue culture seedlings. By the method provided by the invention, the rhododendron tissue culture steps are simplified, the tissue culture cost is reduced, and the ex-vitro rooting rate and the seedling hardening and domesticating survival rate of the tissue culture seedlings are increased.

Description

technical field [0001] The invention relates to a tissue culture rapid propagation method, in particular to a rhododendron tissue culture rapid propagation method. Background technique [0002] The process of tissue culture rapid propagation of rhododendron includes: selection of explants, disinfection, induction of clustered seedlings, subculture, rooting culture and transplanting of test-tube seedlings. Under normal circumstances, the tissue culture of rhododendrons chooses the young stems, stem tips, flower buds or leaves of the current year as explants; the disinfectant for explants generally chooses neutral detergent, mercury chloride, alcohol and NaClO, etc. The disinfection time of each disinfectant should be controlled at an appropriate time to achieve a better disinfection effect. For example, patent CN201010240170 discloses a rapid propagation method of Rhododendron maxima through tissue culture, which uses tissue culture technology to induce callus on the cotyled...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 罗倩罗伟聪董运常
Owner HUAYUAN LANDSCAPE ARCHITECTURE CO LTD
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