Method for preparing cold-soluble Dendrobium officinale extract
A technology of Dendrobium officinale and extract is applied in the field of preparation of cold-dissolving Dendrobium officinale extract, and can solve the problems of different effects of active ingredients, poor water solubility of polysaccharides, and untargeted application of active ingredients, and the like, To achieve the effects of easy absorption by the human body, high extraction rate and simple preparation method
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[0014] A preparation method of cold-soluble dendrobium officinale extract, it comprises the following steps:
[0015] (1) crushing and soaking of Dendrobium candidum: adding hot water to soaking after the dendrobium candidum is pulverized, obtains initial feed liquid;
[0016] (2) Enzymolysis: add cellulase enzymolysis to the primary feed liquid described in step (1), and then add pectinase enzymolysis to obtain secondary feed liquid;
[0017] (3) Filtration: the secondary feed liquid of step (2) gained is filtered to obtain coarse feed liquid;
[0018] (4) Purification: the crude material liquid obtained in step (3) is adsorbed by a macroporous adsorption resin, then eluted with water-ethanol as the eluent, and the effluent is collected;
[0019] (5) concentration: the effluent collected in step (4) is concentrated to obtain a concentrated solution;
[0020] (6) cooling and filtering: the concentrated solution obtained in step (5) is cooled, then filtered, and the filtrate ...
Embodiment 1
[0036] A preparation method of cold-soluble dendrobium officinale extract, it comprises the following steps:
[0037] (1) Crushing and soaking of Dendrobium candidum: first crush the whole herb of Dendrobium candidum to 10 meshes, then add the crushed Dendrobium candidum to 60°C hot water and soak for 30 minutes to obtain the initial material liquid; wherein, the material of Dendrobium candidum and hot water The liquid ratio is 1:10g / mL.
[0038] (2) Enzymolysis: the initial feed solution of step (1) gained is cooled to 40 ℃, then adds the cellulase that accounts for 0.05% of the weight of Dendrobium candidum, and reacts for 45 minutes; After the reaction finishes, add 0.05% of the weight of Dendrobium candidum pectinase, hydrolyzed at 40°C for 60 minutes to obtain secondary feed solution.
[0039] (3) Filtration: The secondary feed liquid obtained in step (2) does not need to be cooled, and is directly firstly filtered through a 40-mesh filter to collect the filtrate; then t...
Embodiment 2
[0045] A preparation method of cold-soluble dendrobium officinale extract, it comprises the following steps:
[0046] (1) Crushing and soaking of Dendrobium candidum: first crush the whole herb of Dendrobium candidum to 15 meshes, then add the crushed Dendrobium candidum to 80°C hot water and soak for 60 minutes to obtain the initial material liquid; wherein, the material of Dendrobium candidum and hot water The liquid ratio is 1:15g / mL.
[0047] (2) Enzymolysis: the initial feed liquid of step (1) gained is cooled to 45 ℃, then adds the cellulase that accounts for 0.1% of the weight of Dendrobium candidum, and reacts for 60 minutes; After the reaction finishes, add 0.1% of the weight of Dendrobium candidum pectinase, hydrolyzed at 45°C for 90 minutes to obtain secondary feed solution.
[0048] (3) Filtration: The secondary feed liquid obtained in step (2) does not need to be cooled, and directly first uses an 80-mesh filter screen for thermal filtration to collect the filtra...
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