Method for preparing cold-soluble Dendrobium officinale extract
A technology of Dendrobium officinale and extract is applied in the field of preparation of cold-dissolving Dendrobium officinale extract, and can solve the problems of different effects of active ingredients, poor water solubility of polysaccharides, and untargeted application of active ingredients, and the like, To achieve the effects of easy absorption by the human body, high extraction rate and simple preparation method
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[0014] A preparation method of cold-dissolving Dendrobium officinale extract, which comprises the following steps:
[0015] (1) Dendrobium officinale is pulverized and soaked: after the Dendrobium officinale is pulverized, add hot water to soak to obtain the initial liquid;
[0016] (2) enzymatic hydrolysis: adding cellulase enzymatic hydrolysis to the initial feed solution described in step (1), and then adding pectinase enzymatic hydrolysis to obtain secondary feed solution;
[0017] (3) filtration: the secondary feed liquid obtained in step (2) is filtered to obtain a crude feed liquid;
[0018] (4) Purification: the crude material liquid obtained in step (3) is adsorbed by macroporous adsorption resin, and then eluted with water-ethanol as eluent, and the effluent is collected;
[0019] (5) concentration: the effluent collected in step (4) is concentrated to obtain concentrated solution;
[0020] (6) filtration by cooling: the concentrated solution obtained in step (5) i...
Example Embodiment
[0035] Example 1:
[0036] A preparation method of cold-dissolving Dendrobium officinale extract, which comprises the following steps:
[0037] (1) Dendrobium candidum pulverization and soaking: first pulverize the whole plant of Dendrobium candidum to 10 meshes, then add the pulverized Dendrobium candidum in hot water of 60 ° C and soak for 30min to obtain the initial liquid; wherein, the material of Dendrobium candidum and hot water is The liquid ratio was 1:10 g / mL.
[0038] (2) Enzymatic hydrolysis: cooling the initial feed solution obtained in step (1) to 40° C., then adding cellulase accounting for 0.05% of the weight of Dendrobium officinale, and reacting for 45 min; after the reaction, adding 0.05% of the weight of Dendrobium officinale The pectinase was enzymatically hydrolyzed at 40 °C for 60 min to obtain secondary feed liquid.
[0039] (3) Filtration: the secondary feed liquid obtained in step (2) does not need to be cooled, and directly firstly uses a 40-mesh fi...
Example Embodiment
[0044] Embodiment 2:
[0045] A preparation method of cold-dissolving Dendrobium officinale extract, which comprises the following steps:
[0046] (1) Dendrobium candidum pulverization and soaking: first pulverize the whole plant of Dendrobium candidum to 15 meshes, then add the pulverized Dendrobium candidum in hot water of 80 ° C and soak for 60min to obtain the initial liquid; wherein, the material of Dendrobium candidum and hot water is The liquid ratio was 1:15 g / mL.
[0047] (2) Enzymatic hydrolysis: the initial feed solution obtained in step (1) is cooled to 45° C., then 0.1% of the cellulase by weight of Dendrobium candidum is added, and the reaction is performed for 60 min; after the reaction, 0.1% of the weight of Dendrobium candidum is added The pectinase was enzymatically hydrolyzed at 45 °C for 90 min to obtain secondary feed liquid.
[0048] (3) Filtration: the secondary feed liquid obtained in step (2) does not need to be cooled, and directly firstly uses an 8...
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