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Biocontrol bacillus velezensis and application thereof in preventing cucumber powdery mildew

A technology of cucumber white powder and bacillus, applied in the field of plant disease prevention and control, can solve the problems of pesticide residues, social problems, short incubation period, etc., achieve protection and restoration of soil micro-ecological environment, reduce the use of pesticides, and have simple preparation methods Effect

Inactive Publication Date: 2018-12-18
天津市农业科学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the short incubation period and rapid onset of the disease, it is necessary to frequently apply pesticides for prevention and control during production. Taking cucumber powdery mildew as an example, it is usually sprayed once every 7-10 days in the spring and autumn seasons when the disease peaks, resulting in serious The problem of pesticide residues not only directly leads to serious economic losses and threatens people's health, but may even lead to serious social problems

Method used

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  • Biocontrol bacillus velezensis and application thereof in preventing cucumber powdery mildew
  • Biocontrol bacillus velezensis and application thereof in preventing cucumber powdery mildew
  • Biocontrol bacillus velezensis and application thereof in preventing cucumber powdery mildew

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Bacillus Velez TB1501 was screened by the Laboratory of Plant Disease Biological Control of Tianjin Institute of Plant Protection, and TB1501 was identified as a strain of Bacillus Velez by the Institute of Microbiology, Chinese Academy of Sciences ( Bacillus Velezensis ), and preserved in the General Microorganism Center of China Committee for the Collection of Microorganisms, the preservation time is: July 6, 2018; the preservation registration number is: CGMCC No. 16069.

[0029] (2) Screening method for Bacillus veleisi TB1501 strain:

[0030] TB1501 strain was isolated from vegetable rhizosphere soil, sampling location: Wuqing District, Tianjin.

[0031] (1) Weigh 1 gram of soil, put it into a 50 mL Erlenmeyer flask, add 9 mL of sterilized tap water, 100 rpm, shake and mix for 30 minutes, and make a soil bacteria suspension;

[0032] (2) Put the soil sample suspension in a water bath at 85°C for 15 minutes, then absorb the supernatant and dilute it 100 time...

Embodiment 2

[0040] Preparation of TB1501 fermentation broth:

[0041] (1) Activate TB1501 strain. Pick a ring of TB1501 colonies, inoculate them in 5 mL of liquid LB medium, and cultivate overnight at 37°C with shaking at 160 rpm to make TB1501 seed liquid.

[0042] (2) Preparation of solid LB medium: 10g / L tryptone, 10g / L sodium chloride, 5g / L beef extract powder, 15g / L agar powder, dissolved in deionized water, sterilized at 121°C for 30 minutes.

[0043] (3) Preparation of liquid LB medium: 10g / L tryptone, 10g / L sodium chloride, 5g / L beef extract powder, dissolved in deionized water, sterilized at 121°C for 30 minutes with damp heat.

[0044] (4) Inoculate 1 / 1000 of the prepared seed liquid into 100 ml of liquid LB medium, 160 rpm, and cultivate for 48-72 hours, which is the prepared TB1501 fermentation liquid.

Embodiment 3

[0046] TB1501 control of cucumber powdery mildew test:

[0047] Spray ×100TB1501 fermentation broth dilution, tebuconazole (provided by Tianjin Hanbang Plant Protective Agent Co., Ltd.), and fuconazole (provided by Tianjin Hanbang Plant Protective Agent Co., Ltd.) at the initial stage of powdery mildew Cucumber leaves were set up as a control with clean water, and the control effect was investigated one week after spraying. The control effect test results show that the TB1501 strain has a good control effect in the control of cucumber powdery mildew, with a control effect of up to 85%, which is better than the chemical drug tebuconazole (provided by Tianjin Hanbang Plant Protective Agent Co., Ltd.) , which is almost equivalent to the new medicament triadol (provided by Tianjin Hanbang Plant Protection Agent Co., Ltd.).

[0048]

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Abstract

The invention relates to a strain of biocontrol bacillus velezensis which is named as TB1501. The strain is preserved in China Microbiological Culture Preservation Management Committee General Microbiological Culture Collection Center, and has the preservation number as CGMCC No.16069 on 6th July, 2018. The strain has a remarkable effect on preventing cucumber powdery mildew. The typical control effect result shows that the TB1501 strain achieves a favorable preventing effect during the process of preventing cucumber powdery mildew, the prevention rate reaches up to 85 percent or more, and thebiocontrol bacillus velezensis is superior to chemical agent tebuconazole and is almost equivalent to novel drug flutriafol.

Description

technical field [0001] The invention relates to the technical field of plant disease prevention and control, in particular to a biocontrol Bacillus veleisi TB1501 and its application in the control of cucumber powdery mildew. Background technique [0002] Powdery mildew, commonly known as "white hair disease", is one of the most important plant diseases in my country at present. Except for conifers, it occurs in various food crops, economic crops, fruits and vegetables, and ornamental plants, and has a wide distribution. It occurs in various planting areas in the country. In the greenhouse cucurbit crops in northern China represented by cucumbers, powdery mildew is particularly harmful and can easily cause large economic losses (up to 50%). Due to the short incubation period and rapid onset of the disease, it is necessary to frequently apply pesticides for prevention and control during production. Taking cucumber powdery mildew as an example, it is usually sprayed once every...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
CPCA01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 田涛孙冰冰石皓文魏军
Owner 天津市农业科学院
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