Tobacco mosaic virus resistant active alkaloid compounds and preparation method thereof

An active alkaloid, mosaic virus technology, applied in the fields of botanical equipment and methods, chemicals for biological control, biocides, etc. difficulties, etc.

Inactive Publication Date: 2018-12-21
YUXI TABACOO COMPANY OF YUNNAN PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Tobacco mosaic virus (TMV), as the first virus discovered and identified in the world, has seriously endangered the yield and quality of tobacco leaves and caused huge economic losses to tobacco farmers.
[0003] Due to the absolute parasiticity of plant viruses to host cells, the diversity of virus transmission modes, and the lack of a complete immune system in plants, it is extremely difficult to develop highly effective antiviral agents, and there is no ideal control agent so far.
At present, the agricultural control measures adopted have relatively large limitations, and chemical control can easily cause environmental problems

Method used

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  • Tobacco mosaic virus resistant active alkaloid compounds and preparation method thereof
  • Tobacco mosaic virus resistant active alkaloid compounds and preparation method thereof
  • Tobacco mosaic virus resistant active alkaloid compounds and preparation method thereof

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Experimental program
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Effect test

Embodiment

[0029] Taking Nicotiana cordata as the test plant, the anti-tobacco common mosaic virus active alkaloid compounds were prepared.

[0030] 1 Materials and methods

[0031] 1.1 Test plants

[0032] The seeds of Nicotiana glutinosa L. were provided by Yunnan Tobacco Agricultural Science Research Institute, and the test plants were collected in Maqiao Research Base, Yuxi City.

[0033] 1.2 Tested virus

[0034] The common strain of tobacco mosaic virus (TMV) U1 was provided by Yunnan Academy of Tobacco Agricultural Sciences. Through conventional friction inoculation, it was propagated on common tobacco K326 (Nicotianatatobacum K326), using Gooding [5] method of purification. Accurately measure 5 μL of purified TMV, dilute it 100 times with 0.01 mol / L phosphate buffer (PB), and measure its mass concentration by ultraviolet spectrophotometer to 16 mg / mL. Store in a -20°C refrigerator, and dilute to 32 μg / mL with 0.01 mol / L PB before use.

[0035] 1.3 The preparation method of ...

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Abstract

The invention relates to tobacco mosaic virus resistant active alkaloid compounds and a preparation method thereof, and belongs to the technical field of biology. An active tracking method is adoptedto obtain the alkaloid compounds with the TMV resistant activity from tobacco, wherein three are new compounds (1-3) [(S)-Nkolbisine-beta-D-glucoside, (R)-Nkolbisine-beta-D-glucoside and Lepabisine A], and nine known compunds (4-12) are firstly separated and obtained from the platymiscium. The alkaloid compounds have the effects of purifying, protecting, treating and inhibiting viral multiplication on the tobacco mosaic virus, the new compound 1 has the stronger virus inhibition effect than the comparator ningnanmycin. One sort of alkaloid compounds with the TMV resistant activity are firstlydiscovered from tobacco, and scientific evidences are provided for further systematic research of tobacco endogenous tobacco mosaic resistant matter.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an active alkaloid compound for resisting tobacco common mosaic virus disease and a preparation method thereof. Background technique [0002] Tobacco mosaic virus (TMV), as the first virus discovered and identified in the world, seriously endangers the yield and quality of tobacco leaves and causes huge economic losses to tobacco farmers. [0003] Due to the absolute parasiticity of plant viruses to host cells, the diversity of virus transmission modes, and the lack of a complete immune system in plants, it is extremely difficult to develop highly effective antiviral agents, and no ideal control agents have been found so far. At present, the agricultural control measures adopted have relatively large limitations, and chemical control is likely to cause environmental problems. Therefore, research and development of anti-TMV natural products are urgently needed. Contents...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D491/048A01N43/90A01P1/00
CPCA01N43/90C07D491/048
Inventor 周文兵李江舟计思贵张立猛崔永和李春林纳红艳黄智华
Owner YUXI TABACOO COMPANY OF YUNNAN PROVINCE
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