Full-automatic magnetic bead method DNA extraction platform and operation method

An operation method and technology of magnetic bead method, applied in the field of DNA extraction, can solve the problems of low DNA extraction precision and purity, many operation steps, slow extraction speed, etc., and achieve the effect of saving the cost of pipette tips, high precision and saving time.

Pending Publication Date: 2018-12-21
江苏齐耀生物科技有限公司
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Problems solved by technology

[0007] The purpose of the present invention is to solve the problems of many operating steps, slow extraction speed, time-consuming and labor-intensive, and low precision and purity of DNA extraction in the DNA fragment extraction process, and to provide an automatic extraction, less time-consuming, simple and convenient operation, The fully automatic magnetic bead method DNA extraction platform with lower cost and higher precision is the operation method

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  • Full-automatic magnetic bead method DNA extraction platform and operation method

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Embodiment Construction

[0019] The present invention will be described in detail below in conjunction with the accompanying drawings and specific examples.

[0020] Such as figure 1 As shown, the platform device of the present invention includes: pipetting module (1), plate-moving module (2), heating and shaking module (3), centrifugal module (4), magnetic separation module (5), orifice plate (6), Test tube (7), gun tip storage area (8), raw material storage area (9), controller (10), described pipetting module (1) adopts high-precision pipetting system, and the CV value of pipetting accuracy is less than 5% ( 100 μl), connected to the controller (10), and operated under the control of the controller (10); the plate moving module (2) adopts a high-precision mechanical arm with a three-dimensional axial positioning accuracy of ±0.2mm, and is used to move the orifice plate (6), connected to the controller (10), and operated under the control of the controller (10); the heating and shaking module (3) i...

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Abstract

The invention discloses a full-automatic magnetic bead method DNA extraction platform and a method. The platform comprises a pipetting module, a plate shifting module, a heating vibration module, a centrifugal module, a magnetic separation module, a pore plate, test tubes, a pipette head storage area, a raw material storage area and a controller. Frequent pipetting is avoided, DNA extraction of 48samples can be performed simultaneously, special pipette heads are adopted for different reagents and materials and can be repeatedly used, the cost of the pipette heads is saved, and cross contamination of the reagents and the materials is also avoided; by means of the controller, automatic sample adding, pore plate transferring, centrifugation and DNA separation are realized, automation of thewhole extraction process of DNA is guaranteed, and the full-automatic magnetic bead method DNA extraction platform which can perform automatic extraction, takes shorter time and is simple to operate,lower in cost and higher in accuracy is provided.

Description

technical field [0001] The invention relates to the field of DNA extraction, in particular to an automatic DNA extraction platform. Background technique [0002] DNA technology plays an increasingly important role in the field of forensic evidence, and the DNA extraction system has become an indispensable experimental facility for today's forensic laboratories. The current laboratory nucleic acid extraction systems mostly use manual or semi-automatic methods, which are cumbersome and complicated, which not only delays the time to solve cases, but also affects the efficiency of case detection. It has increasingly become a bottleneck restricting case detection. The demands of the system are more and more urgent. [0003] In order to ensure the effective extraction of DNA and the use of the extracted DNA in related work, the following principles must be followed for DNA extraction: 1) The primary structure of DNA must be complete; 2) DNA samples should not contain organic solv...

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Application Information

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IPC IPC(8): C12N15/10
CPCC12N15/1013
Inventor 柏锦程张振
Owner 江苏齐耀生物科技有限公司
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