Preparation method of autologous collagen tissue filler matched with face raising surgery through threads
A collagen and matching surface technology, applied in the field of medical cosmetology, can solve problems such as being easily degraded by the body and cannot fundamentally solve the problem of wrinkles, and achieve the effect of improving the adhesion effect, good histocompatibility, and reducing the rejection reaction.
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[0022] An embodiment of the present invention provides a method for preparing an autologous collagen tissue filler for facial thread carving, which is characterized in that it includes the following steps:
[0023] Step 1: Obtain autologous fibroblast tissue, wash it with D-Hanks buffer to remove residual blood and tissue fragments, cut the autologous fibroblast tissue into small pieces, add digestive juice, and put it in a 37°C constant temperature shaking box for digestion for 20 minutes~ After standing for 60 minutes, after the layering, absorb the fibroblast liquid in the upper layer, transfer it into the DMEM medium containing fetal bovine serum, seal and centrifuge, the speed of centrifugation is 800rpm-1800rpm, the centrifugation time is 5min-15min Add an appropriate amount of DMEM medium containing fetal bovine serum to the supernatant, pipette the cells to make a fibroblast suspension, inoculate into culture flasks, and store at 37°C in 5% CO 2 Cultivate at constant t...
Embodiment 1
[0033] Use the following steps to prepare autologous collagen tissue fillers for facial thread carving:
[0034] Step 1: Obtain the autologous fibroblast tissue, wash it with D-Hanks buffer to remove residual blood and tissue fragments, cut the autologous fibroblast tissue into small pieces, add digestive juice, and put it in a 37°C constant temperature shaking box for digestion for 20 minutes. Then let it stand until it is layered, absorb the fibroblast liquid in the upper layer, move it into the DMEM medium containing fetal bovine serum, seal and centrifuge, the speed of centrifugation is 800rpm, the centrifugation time is 15min, remove the supernatant, add again Appropriate amount of DMEM medium containing fetal bovine serum, pipetting cells to make fibroblast suspension, inoculated into culture flasks, at 37°C, 5% CO 2 Culture at a constant temperature, and replace the medium regularly until the fibroblasts cover the bottom of the culture flask;
[0035] Step 2, subcultur...
Embodiment 2
[0041] Use the following steps to prepare autologous collagen tissue fillers for facial thread carving:
[0042] Step 1: Obtain autologous fibroblast tissue, wash it with D-Hanks buffer to remove residual blood and tissue fragments, cut autologous fibroblast tissue into small pieces, add digestive juice, and put it in a 37°C constant temperature shaking box for digestion for 30 minutes. Then let it stand until it is layered, absorb the fibroblast liquid in the upper layer, move it into the DMEM medium containing fetal bovine serum, seal and centrifuge, the speed of centrifugation is 1000rpm, and the centrifugation time is 12min, remove the supernatant, and add Appropriate amount of DMEM medium containing fetal bovine serum, pipetting cells to make fibroblast suspension, inoculated into culture flasks, at 37°C, 5% CO 2 Culture at a constant temperature, and replace the medium regularly until the fibroblasts cover the bottom of the culture flask;
[0043] Step 2, subculture, ta...
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