Micro-pore acellular porcine aorta matrix

Abstract

The invention provides a micro-pore acellular porcine aorta matrix which is characterized in that the micro-pore acellular porcine aorta matrix has a micro-pore structure, a micro-pore of the micro-pore structure is circular or elliptic, and a pig aorta is placed into low-concentration decontaminant buffer solution under a vacuum condition to be repeatedly frozen and thawed and then sequentially oscillated and cleaned in low-concentration decontaminant buffer solution, sterile deionized water and phosphate solution to obtain the micro-pore acellular porcine aorta matrix. The micro-pore acellular porcine aorta matrix effectively retains a three-dimensional structure and biomechanical property of a porcine aorta tissue, toxicity of a decontaminant does not remain, infiltrative growth of fibroblasts and vascular endothelial cells can be promoted, so that regeneration of blood vessels and collagen is promoted, and the matrix has excellent in-vivo remodeling performance.

Description

technical field [0001] The invention relates to the field of tissue engineering, in particular to a microporous decellularized porcine aortic matrix. Background technique [0002] Vascular diseases represented by aortic aneurysm, vascular dissection, and atherosclerosis are not only one of the leading fatal diseases in developed countries, but also in developing countries. Surgical replacement of diseased blood vessels is the last resort in the treatment of vascular diseases, thus various vascular replacement materials are required. In addition, as the incidence of esophageal cancer increases year by year, the demand for artificial esophageal replacement materials is also increasing. [0003] Although homogeneous tissue replacement is an ideal replacement material, there are few sources and ethical restrictions. At present, with the development of biotechnology, a variety of polymer biosynthetic materials have been developed as artificial tissue replacement materials. For...

AI Technical Summary

Problems solved by technology

In the prior art, detergents are generally used to treat porcine aorta to achieve the purpose of decellularization. Although this method can achieve a good decellularization effect, the decellularized porcine aorta matrix obtained by this method often has residual toxicity, and Its biomechanical properties are also compromised

In addition, a large number of studies have shown that the extracellular matrix structure of decellularized porcine aorta is dense, so it is difficult for cells to grow endogenously when applied in the host body, and the remodeling effect in the host body is not good

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