The invention discloses a noniron finishing method of a silk fabric. The noniron finishing method particularly comprises the following steps: moistening and rinsing the silk fabric with warm water twice, refining and expanding the silk fabric by using a biological enzyme, after rinsing with warm water, padding the expanded silk fabric in a wash and wear finisher containing silk fibroin, citric acid and chitosan quaternary ammonium salt, then predrying the silk fabric, tentering at constant temperature, baking at high temperature, washing with water and drying, and pressing-packaging to obtain the noniron finished silk fabric. According to the noniron finishing method, a biological enzyme technology is combined with a padding technology, the silk fabric is subjected to noniron finishing by using the silk fibroin, the citric acid and the chitosan quaternary ammonium salt, so that the noniron finishing method has the advantages of simple and effective, environmental-friendly and biological effects. The silk fabric prepared by adopting the noniron finishing method reaches a noniron degree, and has a good crease-resisting and deformation-resisting capabilities; the dyeing performance of the fabric is improved; the handfeel, the style and the mechanical property of the fabric are reserved, and thus the noniron finishing method is an ideal noniron finishing method of the silk fabric.