Composite stent as well as preparation method and application thereof

A technology of composite scaffold and drug-loaded microspheres, which is applied in the fields of biomedical engineering and biomedical materials, can solve the problems that the microspheres are not suitable for bone repair, and the combination of drug-loaded microspheres and scaffold materials is not firm, etc., so as to improve the bonding stability. , Good drug-loading and drug-releasing properties, and the effect of improving osteoinductive effect

Active Publication Date: 2021-04-02
GUANGDONG PROV MEDICAL INSTR INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the shape and size of the microspheres themselves, the microspheres are not suitable for bone repair alone
Since the technology of drug-loaded microspheres has been studied, its in vivo performance research is inseparable from the compounding with gel system or scaffold material, and the composite scaffolds containing microspheres prepared in the prior art usually have the combination of drug-loaded microspheres and scaffold materials. Weak joint problem

Method used

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  • Composite stent as well as preparation method and application thereof
  • Composite stent as well as preparation method and application thereof
  • Composite stent as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0025] (1) Preparation of mesoporous calcium silicate

[0026] The mesoporous calcium silicate used in the following composite scaffold preparation process is obtained by the following method:

[0027] Dissolve 6.8g of cetyltrimethylammonium bromide and 12ml of ammonium hydroxide in 600mL of deionized water, add 30mL of ethyl orthosilicate and 31.5g of calcium nitrate tetrahydrate sequentially at intervals of 30min under stirring at 40°C, and add completely Afterwards, stirring was continued for 3 h, the product was collected by filtration, and washed three times with deionized water and ethanol respectively; then, the collected powder was dried overnight at 60°C and calcined at 600°C for 2h to obtain mesoporous calcium silicate.

[0028] (2) Preparation of composite scaffold

Embodiment 1

[0030] A kind of composite bracket, its preparation method comprises the following steps:

[0031]S1. Preparation of acellular matrix, including: taking 4-week-old dairy cow upper limb subchondral bone trabeculae, washing with high-pressure water; decellularizing with PBS buffer solution containing 0.1% ethylenediaminetetraacetic acid at room temperature for 1 hour, containing 0.25 Soak in 10mM Tris buffer of % sodium dodecyl sulfate for 7h, wash with PBS several times until there are no bubbles; then use supercritical carbon dioxide technology to treat it, set the minimum pressure to 8MPa, the maximum pressure to 18MPa, and the system temperature to 37 ℃, specifically exposed to the maximum pressure for 10 minutes, with a flow rate of 10kg / h, and then reduced to normal pressure within 10 minutes to prepare an acellular matrix with a three-dimensional porous structure as a scaffold matrix;

[0032] S2, the preparation of drug-loaded microspheres, comprising: mixing 10mgVEGF an...

Embodiment 2

[0035] A kind of composite bracket, its preparation method comprises the following steps:

[0036] S1. Preparation of acellular matrix, comprising: taking 16-week-old dairy cow upper limb subchondral bone trabeculae, washing with high-pressure water; Soak in 0.1% sodium dodecyl sulfate in 10mM Tris buffer for 10h, wash with PBS several times until there are no bubbles; then use supercritical carbon dioxide technology to treat it, set the minimum pressure to 7MPa, the maximum pressure to 20MPa, and the system temperature to be 37°C; Specifically, exposed to the maximum pressure for 15 minutes, with a flow rate of 10kg / h, and then lowered to normal pressure within 5 minutes to prepare an acellular matrix with a three-dimensional porous structure as a scaffold matrix;

[0037] S2. Preparation of drug-loaded microspheres, including: mixing 10 mg of BMP-7 with 40 mg of mesoporous calcium silicate to obtain a mixed powder of drug and mesoporous calcium silicate, and then dispersing ...

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Abstract

The invention discloses a composite stent as well as a preparation method and application thereof. The composite stent comprises a stent matrix, drug-loaded microspheres and an alginate layer; the stent matrix is of a three-dimensional porous structure; the drug-loaded microspheres are fixed to the surface and pore channels of the stent matrix; the alginate layer covers the surfaces of the drug-loaded microspheres and the stent matrix; drug-loaded microspheres comprise carrier microspheres and drugs loaded on the carrier microspheres; the drugs consist of a bone repair medicine, and/or a growth factor; and the raw materials of the carrier microspheres are composed of degradable polyester and mesoporous calcium silicate. The composite stent is stable in structure, and has good drug slow releasing effect as well as osteogenic differentiation capacity; and moreover, the composite stent is capable of effectively promoting repair and reconstruction of bone tissue.

Description

technical field [0001] The invention relates to the technical fields of biomedical engineering and biomedical materials, in particular to a composite bracket and its preparation method and application. Background technique [0002] With the continuous development of medicine, pharmacy, and biology, new drugs for various diseases emerge in an endless stream, but how to release drugs continuously and stably in the body is still a difficult problem. Regardless of oral administration or intravenous injection, there will be a "peak-valley" phenomenon in the change of blood drug concentration. If the drug concentration is too high, it will cause relatively large toxic and side effects, while if it is too low, the therapeutic effect will not be achieved. Although the emerging bioactive macromolecular drugs are highly effective, they have a short biological half-life and are easily inactivated, which is also a problem that plagues drug developers. Use appropriate biomaterials to em...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/02A61L27/18A61L27/22A61L27/34A61L27/36A61L27/54A61L27/56A61L27/58
CPCA61L27/56A61L27/54A61L27/58A61L27/227A61L27/18A61L27/025A61L27/34A61L27/3608A61L27/3687A61L2300/414A61L2300/622C08L5/04
Inventor 许为康王丽艳
Owner GUANGDONG PROV MEDICAL INSTR INST
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