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Method for simultaneously quantitatively detecting astragaloside-IV and cycloxanthine in mouse plasma

A technology of astragaloside IV and cycloastragaloside, which is applied in the field of medicine, can solve the problems of low solubility, low bioavailability, high relative molecular mass and the like, and achieves the effect of high specificity

Active Publication Date: 2018-12-25
MINZU UNIVERSITY OF CHINA
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  • Application Information

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Problems solved by technology

The other method takes a long data acquisition time (17 minutes)
In addition, the sample volume of the above two methods is 50 μL plasma, which is not suitable for quantification in mouse plasma
In addition, due to the high relative molecular mass and low solubility of AST, the bioavailability in animals is not high

Method used

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  • Method for simultaneously quantitatively detecting astragaloside-IV and cycloxanthine in mouse plasma
  • Method for simultaneously quantitatively detecting astragaloside-IV and cycloxanthine in mouse plasma
  • Method for simultaneously quantitatively detecting astragaloside-IV and cycloxanthine in mouse plasma

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Experimental program
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Embodiment

[0052] 1.1 Experimental reagents

[0053] Astragaloside IV (Astragaloside IV, AST), molecular formula C 41 h 68 o 14 , with a relative molecular mass of 784.4609, purchased from China National Institute for the Control of Pharmaceutical and Biological Products; cycloastragenol (Cycloastragenol, CST), molecular formula C 30 h 50 o 5 , relative molecular mass 490.3658, internal standard Digoxin (Digoxin), molecular formula C 41 h 64 o 14 , with a relative molecular mass of 780.4296, all purchased from Chengdu Kangbang Biotechnology Co., Ltd., with a purity of >98% by HPLC, sealed and protected from light, and stored at 2-8°C. Acetonitrile, methanol, (chromatographically pure) were purchased from Merck, Germany, and formic acid (chromatographically pure) was purchased from Roe, USA. The experimental water was Wahaha purified water.

[0054] 2.2 Plasma sample collection

[0055] Twenty-four male KM mice (8 weeks old, weighing 35-40 g) were purchased from Beijing Weitong ...

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Abstract

The present invention establishes a simultaneous quantification method for mouse plasma based on UPLC-HRMS, in which the method targets astragaloside IV and cycloxanthine that is the main metabolite of astragaloside IV. The quantitative time of the method is 3 mins, digoxin is used as an internal standard, and only 20 [mu]L of mouse plasma is needed, thus having the advantages of rapidity, high sensitivity and strong specificity. After being precipitated by the protein, the sample is filtered by dephospholipidation plate, which effectively reduces the matrix effect of endogenous metabolites ofphospholipids in plasma on the analyte. Ultra-high performance C18 column is used as the analytical column to detect two kinds of analytes and internal standards in the electrospray ion source positive ion selective ion monitoring mode. The linear range of the two analytes is 1-200ng / mL, the intra-day and inter-day precision is <=8.6%, and the precision is <=8.8%, which indicates that the methodhas good precision and accuracy. The method for simultaneously quantitatively detecting astragaloside-IV and cycloxanthine in mouse plasma was successfully applied to the pharmacokinetic study of astragaloside IV mice.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to the establishment of a simultaneous quantitative method for astragaloside IV and cycloastragenol in mouse plasma based on UPLC-HRMS and the application of pharmacokinetics. Background technique [0002] Astragaloside-IV (Astragaloside IV, AST), as the main active ingredient of Chinese herbal medicine Astragalus, has various pharmacological effects, including anti-inflammatory, anti-hypertensive, cardioprotective, anti-oxidative and anti-apoptotic. AST has also been reported as a potential therapeutic drug for various metabolic syndromes. The main bioactive metabolite of AST is Cycloastragenol (CST), which is a small molecule telomerase activator and a potential inhibitor of adipogenesis. In addition, recent studies have shown that AST and CST have equal efficacy in inhibiting reactive oxygen species (Reactive oxygen species, ROS)-related endoplasmic reticulum stress and inhib...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/7266
Inventor 王中华何秉淑陈路路再帕尔·阿不力孜
Owner MINZU UNIVERSITY OF CHINA
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