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A method for screening turbot feed protectant

A technology of turbot and protective agent is applied in the field of screening the feed protective agent of turbot, which can solve the problems of declining body health, water environment pollution, water quality deterioration, etc., so as to improve health, ensure systemicity, and reduce the occurrence of diseases. Effect

Active Publication Date: 2022-05-17
SHANDONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The decline of intestinal health not only leads to a decline in the health of the body, but also reduces the digestion and absorption rate of nutrients. A large amount of undigested nutrients are discharged into the water body, causing water environmental pollution and water quality deterioration, which in turn reduces the health of fish and causes frequent outbreaks of diseases.

Method used

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  • A method for screening turbot feed protectant
  • A method for screening turbot feed protectant
  • A method for screening turbot feed protectant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: Screening of detection index

[0017] The applicant found that substituting soybean meal for fish meal in the feed would lead to a decline in the intestinal health of turbot; by adding soybean meal to the feed, a sub-health model of the turbot intestinal tract was established, and the effect of setting indicators on the response to turbot intestinal health was investigated. The sensitivity of the health level of the road is determined to determine the index used for the screening of turbot feed protectant. The screening method of the index is as follows:

[0018] The whole fish meal feed was used as the control feed, and the feed with 40% soybean meal instead of fish meal was used as the experimental feed, and two different feeds were fed to the cultured fish (Table 1). The breeding facilities use 300L fiberglass barrels, and 30 turbot fry are stocked in each barrel, with a starting weight of about 7.5 grams. Each barrel was used as a replicate, and each ...

Embodiment 2

[0049] Example 2 Detection of the protective effect of mannan oligosaccharides on turbot intestinal health

[0050] The feed that replaced fish meal with soybean meal was used as the control feed, and the intestinal protective agent to be investigated was added to the control feed as the experimental feed, and two different feeds were fed to the farmed fish. The breeding facilities use 300L fiberglass barrels, and 30 turbot fry are stocked in each barrel, with a starting weight of about 7.5 grams. Each barrel was used as a replicate, and each feed was set up three replicates. Running water farming, inflatable. During the 56-day breeding test, the dissolved oxygen in seawater was 7.8-8.2mg / L, pH 7.5-8.0, salinity 28-30, and temperature 14-16°C. They were fed twice a day, at 07:00 and 19:00 respectively, and they were fed with enough food. The breeding cycle is 8 weeks.

[0051] The feed with soybean meal instead of fish meal was used as the control feed (Table 3), and 0.2% ...

Embodiment 3

[0062] Example 3: Detection of the protective effect of dextran on turbot intestinal health

[0063] The feed with soybean meal instead of fish meal was used as the control feed (Table 5), and 0.2% dextran was added to the control feed as the experimental feed, and two different feeds were fed to the cultured fish. Feeding management, sample collection, and index determination are the same as the above-mentioned methods.

[0064] Table 5: Feed raw material composition and ingredient list

[0065]

[0066] The experimental results are as follows:

[0067] Table 6: Effects of dextran on intestinal health evaluation indicators of turbot

[0068]

[0069]

[0070] Note: * Indicates the percentage change in the mean value of the experimental diet compared to the mean value of the control diet

[0071] It can be seen from Table 6 that the addition of dextran in the feed can significantly increase the intestinal body ratio of turbot and the height of the absorption vac...

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Abstract

The present invention provides a method for screening turbot feed protectant, which can effectively screen the protectant effective for turbot intestinal health and its addition amount. The present invention can be used for the screening of fish enteritis protective agent, through the investigation of three different levels of tissue, cell and molecule, combined with the intestinal physiological characteristics and intestinal tissue characteristics of fish, the systematic and comprehensive evaluation method is guaranteed The obtained results can provide a basis for improving fish gut health and reducing disease occurrence.

Description

technical field [0001] The invention belongs to the technical field of aquaculture feed, in particular to a method for screening a feed protectant for turbot. Background technique [0002] Fish provides high-quality protein sources for humans, which is very important for improving dietary nutritional structure and ensuring human health. With the development of society, the improvement of people's living standards and the increasing awareness of health, the demand and consumption of fish products have increased dramatically. However, while fish farming is booming, the problems of water pollution and ecological environment damage are becoming increasingly prominent, resulting in frequent outbreaks of fish diseases. The reason is that fish live in water and are lower vertebrates with weak digestion and disease resistance. Among them, intestinal health is the key to healthy fish farming. The intestinal tract is the organ with the largest contact area between fish and the exter...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K50/80A01K61/10
CPCA01K61/10A23K50/80Y02A40/81
Inventor 谷珉柳文爽白楠贾倩潘世会
Owner SHANDONG UNIV
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