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Extraction method for bradyrhizobium extract and application of bradyrhizobium extraction in mango grafting

A technology of brachyrhizobium and extraction method, which is applied in microorganism-based methods, biochemical equipment and methods, and botanical equipment and methods, etc., can solve the problem of low graft survival rate, low utilization rate of brachyrhizobia research, and grafting. The interface healing effect is not ideal and other problems, to achieve the effect of promoting the healing of scabs, promoting growth and development, and shortening the healing time

Active Publication Date: 2019-01-04
云南元江大有为食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention overcomes the low utilization rate of bradyrhizobium in the prior art, the unsatisfactory effect of the existing plant healing agent on promoting the healing of the grafting interface, and the low survival rate of grafting, and provides a kind of bradyrhizobium extract. Extraction method and its application in mango grafting

Method used

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  • Extraction method for bradyrhizobium extract and application of bradyrhizobium extraction in mango grafting

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A method for extracting Bradyrhizobium extract, comprising the steps of:

[0020] (1) Selection and cultivation of Bradyrhizobium: select Bradyrhizobium from the roots of peanuts, then dry and pulverize the peanut roots, use ethanol for rough extraction to obtain crude extracts, and then perform supercritical carbon dioxide extraction on the crude extracts, The crude extract is placed in a supercritical extraction ax for supercritical extraction, and an equal mass ratio of ethanol, ethyl acetate, and n-butanol mixture is used as an entrainer to extract for 20 minutes to obtain the Bradyrhizobium extract; the supercritical The carbon dioxide extraction temperature is 35°C, the extraction pressure is 35kpa, and the carbon dioxide flow rate is 25L / min. The selected bradyrhizobium was placed in the culture medium, cultured at 28° C. for 5 days to obtain a culture solution, and the culture solution was filtered and sterilized by gauze to obtain a bradyrhizobium fermentation ...

Embodiment 2

[0023] A method for extracting Bradyrhizobium extract, comprising the steps of:

[0024] (1) Selection and cultivation of Bradyrhizobium: select Bradyrhizobium from the roots of peanuts, then dry and pulverize the peanut roots, use ethanol for rough extraction to obtain crude extracts, and then perform supercritical carbon dioxide extraction on the crude extracts, The crude extract is placed in a supercritical extraction ax for supercritical extraction, and an equal mass ratio of ethanol, ethyl acetate, and n-butanol mixture is used as an entrainer to extract for 20 minutes to obtain the Bradyrhizobium extract; the supercritical The carbon dioxide extraction temperature is 35°C, the extraction pressure is 35kpa, and the carbon dioxide flow rate is 25L / min. The selected bradyrhizobium was placed in the culture medium, cultured at 25° C. for 6 days to obtain a culture solution, and the culture solution was filtered and sterilized by gauze to obtain a bradyrhizobium fermentation ...

Embodiment 3

[0027] A method for extracting Bradyrhizobium extract, comprising the steps of:

[0028] (1) Selection and cultivation of Bradyrhizobium: select Bradyrhizobium from the roots of peanuts, then dry and pulverize the peanut roots, use ethanol for rough extraction to obtain crude extracts, and then perform supercritical carbon dioxide extraction on the crude extracts, The crude extract is placed in a supercritical extraction ax for supercritical extraction, and an equal mass ratio of ethanol, ethyl acetate, and n-butanol mixture is used as an entrainer to extract for 20 minutes to obtain the Bradyrhizobium extract; the supercritical The carbon dioxide extraction temperature is 35°C, the extraction pressure is 35kpa, and the carbon dioxide flow rate is 25L / min. The selected bradyrhizobium was placed in the culture medium, cultured at 26° C. for 5 days to obtain a culture solution, and the culture solution was filtered and sterilized by gauze to obtain a bradyrhizobium fermentation ...

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Abstract

The invention discloses an extraction method for bradyrhizobium extract and application of the bradyrhizobium extract in mango grafting. The extraction method comprises the steps of selective culturing of bradyrhizobium: specifically selecting bradyrhizobium from peanut roots, drying the peanut roots, conducting crushing, conducting crude extraction with ethanol to obtain a crude extract, then putting the crude extract in a supercritical extraction kettle to conduct supercritical carbon dioxide extraction on the crude extract, so as to obtain a bradyrhizobium extraction substance; (2) puttingselected bradyrhizobium in a culture medium for culturing to obtain a culture solution, filtering the culture solution by a gauze for sterilization, so as to obtain a bradyrhizobium fermentation solution; and (3) mixing the bradyrhizobium extraction substance and the bradyrhizobium fermentation solution to obtain the bradyrhizobium extract. According to the invention, an agent for promoting graftwound to heal is prepared by mixing the bradyrhizobium extraction substance and the bradyrhizobium fermentation solution, is capable of promoting healing of grafting wounds of mangoes, and guaranteesa short healing time for the grafting wounds and a high grafting successful rate.

Description

technical field [0001] The invention relates to the technical field of fruit tree planting, in particular to a method for extracting a bradyrhizobium extract and its application in mango grafting. Background technique [0002] At present, grafting technology is widely used in horticultural crops. In production practice, the factors affecting the survival of grafted seedlings are not only the affinity of rootstock and ear, but also the nutritional status of rootstock and ear, moisture, sterilization and other factors, which affect the healing speed of grafted joints. In order to ensure the survival rate of grafting, people apply various chemical substances to the grafting to promote the healing of the grafting wound as soon as possible, and the effect is more obvious. In the existing research, the components that have been grafted into the interface healing agent mainly contain plant growth regulators such as naphthalene acetic acid, indole acetic acid and gibberellin, bacte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G2/30A01G2/38A01G7/06C12N1/20C12R1/41
CPCA01G2/30A01G2/38A01G7/06C12N1/20
Inventor 韦绍仙
Owner 云南元江大有为食品有限公司
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