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Detection method and application of personalized children's drug analysis system based on SNP (single nucleotide polymorphisms) sites

A detection method and site technology, which is applied in the detection field of children's personalized drug analysis, can solve the problems of high cost and low detection efficiency, and achieve the effects of low cost, low detection cost and high throughput

Active Publication Date: 2019-01-04
深圳一道医学检验实验室
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Problems solved by technology

[0009] At present, the research on the polymorphism of these gene loci is mainly based on the Sanger sequencing method, which can only detect a certain region of a certain sample, and the detection efficiency is low and the cost is high

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  • Detection method and application of personalized children's drug analysis system based on SNP (single nucleotide polymorphisms) sites
  • Detection method and application of personalized children's drug analysis system based on SNP (single nucleotide polymorphisms) sites
  • Detection method and application of personalized children's drug analysis system based on SNP (single nucleotide polymorphisms) sites

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Embodiment Construction

[0030] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Apparently, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0031] In order to better understand the present invention, definitions and explanations of relevant terms are provided below.

[0032] The term "high resolution melting curve method" as used herein mainly refers to the difference based on the physical properties of nucleic acid molecules. The fragment length, GC content, and GC distribution of different nucleic acid molecules are different, so any double-stranded DNA molecule will have its own shape and position of the melting...

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Abstract

The invention discloses a detection method personalized children's drug analysis system based on SNP (single nucleotide polymorphisms) sites. SNP site combination for common anti-infection children'sdrug risk evaluation comprise 67 SNP site combinations and specific primers for detecting drug toxicity, drug metabolism and medicine reactions. Sample DNA is subjected to PCR amplification through site specific primers, the difference between a melting curve and a standard melting curve is analyzed, the Tm values of PCR amplified products are compared, and corresponding genotyping results can beobtained. The detection method can be applied to quick screening of applicability of common drugs to children, provides guide for reasonable selection of children's drugs and has the advantages of being simple to operate, high in efficiency, high in specificity, economical and the like.

Description

technical field [0001] The invention relates to the technical field of gene detection, in particular to a detection method and application thereof for the analysis of individualized medication for children based on SNP sites. Background technique [0002] Drug gene detection is to extract the DNA in the patient's oral mucosa, interpret the genes related to the drug response, and predict the degree of adverse reactions of the patient to the specific drug according to the statistical results of the clinical response of different genotypes to the specific drug. to a certain guiding role. [0003] The 2017 "National Adverse Drug Reaction Monitoring Annual Report" pointed out that a total of 1.43 million adverse drug reactions or events were received throughout the year, of which children under the age of 14 (inclusive) accounted for 9.9% of the total reports, and serious reports accounted for 9.9% of the total reports. 6.5% of the total. In 2017, among the distribution of drug...

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/6858
CPCC12Q1/6858C12Q1/6883C12Q2600/106C12Q2600/156C12Q2600/16C12Q2531/113C12Q2563/107C12Q2537/143
Inventor 欧阳秦
Owner 深圳一道医学检验实验室
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