Microorganisms and artificial ecosystems for the production of protein, food, and useful co-products from c1 substrates

A technology of microorganisms, autotrophic microorganisms, used in biological regeneration and closed-loop life support systems, production of amino acids, proteins and other biomass components, the field of microbial amino acids

Pending Publication Date: 2019-01-04
KIVERDI INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This would result in feedstock flexibility far beyond comparable heterotrophic systems

Method used

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  • Microorganisms and artificial ecosystems for the production of protein, food, and useful co-products from c1 substrates
  • Microorganisms and artificial ecosystems for the production of protein, food, and useful co-products from c1 substrates
  • Microorganisms and artificial ecosystems for the production of protein, food, and useful co-products from c1 substrates

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0347] Insecticidal Cuspermia strain DSM 531 in H as the only energy and carbon source for growth 2 and CO 2 and O 2 Grow on mixture of gases.

[0348] Follow the protocol below for experiments using gas mixtures in airtight serum bottles.

[0349] Experimental inoculum: 5% by volume, taken from another H 2 Growing serum bottle cultures.

[0350] to the initial H 2 Serum bottle cultures grown were given a 5% inoculum from a lysogenous broth (LB) grown C. insecticidal inoculum and were incubated in H 2 / CO 2 / O 2 Growth on the gas mixture was about 72 hours. Inoculum of primary LB growth was recovered from glycerol stock stored at -80 °C.

[0351] Serum bottle growth on gas was performed in 160 ml stoppered and sealed Wheaton glass serum bottles (VWR product number 16171-385). The volume of liquid medium was 20 ml. Bottles were stoppered with rubber stoppers (VWR#100483-774) and aluminum seals (VWR#89047-008) using a Wheaton hand crimper (VWR#80078-996). 20ml workin...

example 2

[0360] Insecticidal copper greedy bacteria strain DSM 531 in H as the only energy and carbon source for growth 2 , CO 2 and O 2 Grow on the gas mixture to a dry cell density of 38 g / L.

[0361] Follow the protocol below for use in stirred tank bioreactors including H 2 , CO 2 and O 2 Experiments with gas mixtures.

[0362] Setup: Cultures were grown in batches using custom-made 500 mL glass fermenters with PEEK top plates. Temperature and pH were controlled and monitored with a commercial controller (Electrolab, Fermac 360, UK). A combination of a magnetic stir bar and a continuous cycle of 280 mL / min was used for mixing. Recirculation can be taken from the bottom liquid portion of the reactor and returned to the headspace via spargers to control foaming or run in reverse to recycle headspace gas and foam to the bottom of the broth. The gas supply comes from compressed H 2 , compressed CO 2 and room air, each regulated to 20psi. H 2 And air is delivered to a flow p...

example 3

[0371] The ability of microorganisms from the genera Rhodococcus and Cuprocorcus to grow on different carbon sources was tested ( Figure 7 ). Colonies of strains grown on LB agar plates at 30°C were transferred to flasks containing 10% (V / V) of the indicated media at 30°C and 250 rpm for 3-20 days. Rhodococcus opacus strain DSM 44193 exhibited only in MSM medium (1L medium A: 9g Na 2 HPO 4 .12H 2 O, 1.5g H 2 PO 4 , 1.0g NH 4 Cl and 0.2g MgSO 4 .7H 2 O / 1L; 10ml medium B: 50mg ferric ammonium citrate and 100mg CaCl 2 / 100ml; 10ml Medium C: 5g NaHCO 3 / 100ml; and 1ml trace mineral solution: 100mg ZnSO 4 .7H 2 O, 30mg MnCl 2 .4H 2 O, 300mg H 3 BO 3 , 200mgCoCl 2 .6H 2 O, 10mg CuCl 2 .2H 2 O, 20mg NiCl 2 .6H 2 O and 30 mg Na 2 MoO 4 .2H 2 0 / 1 L) under heterotrophic growth conditions measured by optical density (OD) at 650 nm. Rhodococcus opacus strain DSM 43205 showed the same growth rate under heterotrophic conditions, reaching OD=9.0. Strain DSM 43205 w...

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Abstract

Microorganisms and bioprocesses are provided that convert gaseous C1 containing substrates, such as syngas, producer gas, and renewable H2 combined with CO2, into nutritional and other useful bioproducts.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of U.S. Patent Application No. 62 / 310,705, filed March 19, 2016, and U.S. Patent Application No. 62 / 454,347, filed February 3, 2017, both of which are incorporated herein by reference in their entirety middle. technical field [0003] The present subject matter involves the use of gaseous substrates such as synthesis gas or process gas or pyrolysis gas or H 2 with CO 2 The gas mixture serves as a carbon and energy source for the bioproduction of amino acids and proteins and other biomass components in microbial systems. The invention also relates to the use of microbial amino acids, proteins and other biomass components for feeding or providing nutrition to other heterotrophic organisms, animals or humans. Amino acids, proteins and other biomass components produced according to the present invention can be consumed by other organisms and used as nutrients for the production of food...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/04C12P5/00C12P7/64C25B1/04C12M1/04C12N1/20
CPCC12N1/20C12P1/04C12P5/00C12P7/64C25B1/04C12P21/02C12P13/04Y02P20/133A61P35/00Y02E50/30Y02E60/36Y02W30/40C07K16/2896G01N33/573G01N33/84C07K2317/71C07K2317/92C07K2317/76C07K2317/52C07K2317/24G01N2333/916C12P7/6409C12P17/167C12P13/001C12P25/00
Inventor J.S.里德J.盖勒S.汉德
Owner KIVERDI INC
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