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Phenyllactic acid composite biological preservative and preparation method thereof

A technology of biological preservatives and phenyllactic acid, which is applied to the preservation of bacteria and food ingredients used in food preparation as anti-microbial preservation, lactobacilli, etc., can solve the problem of low antibacterial activity of fermentation broth, difficulty in separation and purification of phenyllactic acid, and limitations. Research and application of phenyllactic acid

Active Publication Date: 2019-01-11
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But at present, the yield of phenyllactic acid by microbial conversion method is low, the antibacterial activity of fermentation broth is not high, and the separation and purification of phenyllactic acid are difficult, which limits the research and application of phenyllactic acid

Method used

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  • Phenyllactic acid composite biological preservative and preparation method thereof
  • Phenyllactic acid composite biological preservative and preparation method thereof
  • Phenyllactic acid composite biological preservative and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0023] (1) Strain fermentation

[0024] Lactic acid bacteria are added to a liquid containing phenylalanine for fermentation to produce phenyllactic acid. The lactic acid bacteria is Lactobacillus plantarum, the initial inoculation amount is 2%, the initial pH is 6.4, the addition amount of phenylalanine is 3.470g / L, the fermentation temperature is 37°C, and the fermentation time is 48h to prepare phenyllactic acid fermentation broth.

[0025] (2) Fermentation broth pretreatment

[0026] Adjust the pH of the phenyllactic acid fermentation liquid prepared in the above step (1) to 6.5-7.0 with 10mol / L sodium hydroxide under agitation to fully release the phenyllactic acid in the fermentation liquid, and then pass through a 150-300,000 pore diameter The filter membrane removes macromolecular proteins and impurities, and the filtrate is collected.

[0027] (3) Compounding of fermentation broth

[0028] Get the phenyllactic acid fermentation broth filtrate that step (2) obtains,...

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Abstract

The invention discloses a phenyllactic acid composite biological preservative and a preparation method thereof. The composite biological preservative comprises a phenyllactic acid fermentation liquid,an antioxidant accounting for 0.1 to 1% the weight of the phenyllactic acid fermentation liquid and a bacterial quorum sensing inhibitor accounting for 0.1 to 0.5% the weight of the phenyllactic acidfermentation liquid, wherein the bacterial quorum sensing inhibitor is a mixture of one or more selected from a group consisting of cinnamaldehyde, naringin and dandelion extract. The preservative ofthe invention overcomes the limitation of a narrow antibacterial spectrum of simple phenyllactic acid, has broad-spectrum anticorrosion and anti-oxidation effect and is capable of interfering with bacterial quorum sensing and blocking information exchange between spoilage bacteria, thereby improving the sensitivity of the spoilage bacteria to the biological preservative and fundamentally enhancing the anticorrosion effect of phenyllactic acid.

Description

technical field [0001] The invention belongs to the field of food additives and food preservation, and in particular relates to a phenyllactic acid composite biological preservative and a preparation method thereof. Background technique [0002] Phenyllactic acid, also known as 2-hydroxy-3-phenylpropionic acid, has the molecular formula C 9 h 10 o 3 . The relative molecular mass of phenyllactic acid is 166.17, and the high-purity anhydrous phenyllactic acid is usually in the form of white crystals with relatively stable properties. As a naturally occurring bacteriostatic compound and an important organic acid, phenyllactic acid has many uses. Since phenyllactic acid was first isolated from honey and fermented foods, it has become a natural, green alternative to chemical preservatives. The melting point of phenyllactic acid is 121-125°C, and it is not easily destroyed at 121°C for 20 minutes. Phenyllactic acid has good solubility in water and can adapt to a wide pH rang...

Claims

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Application Information

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IPC IPC(8): A23L3/3508A23L3/3499A23L3/3562A23L3/3472A23L3/349A23B4/20
CPCA23B4/20A23L3/3472A23L3/349A23L3/3499A23L3/3508A23L3/3562A23V2002/00A23V2400/169A23V2200/10A23V2200/02A23V2250/0638A23V2250/1614A23V2250/21A23V2250/2132A23V2250/214
Inventor 曾名湧史国萃刘尊英
Owner OCEAN UNIV OF CHINA
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