Method for preparing enzyme from brewer's spent grains
A technology of wort ferment and ferment, applied in the field of bioengineering, can solve the problems of neglect of nutritional value and lack of wort ferment, and achieve the effects of benefiting human health, improving utilization value and good antioxidant effect.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0030] (1) Yeast expansion
[0031] Preparation of culture medium (wort): take 2kg of malted malt for saccharification to prepare wort. The saccharification process is as follows: figure 1 shown. Filter after saccharification, remove wheat grains, boil the filtered clear liquid for 60 minutes, and add 0.03% hops during boiling (add 1 / 2 at the beginning of boiling, add 1 / 4 at the time of 30 minutes, add 1 / 4 at the time of 50 minutes) 4). After filtering, the wort was subpackaged and sterilized at 105°C for 10 minutes for later use.
[0032] Take 100 μL of strains Saccharomyces cerevisiae and Saccharomyces cerevisiae stored in glycerol tubes and put them in 10mL of wort, culture at 28°C and 180r / min for 12h, transfer 10mL of the culture solution to 200mL of fresh wort for 24h at 28°C and 180r / min, and take the culture solution in 50mL In a centrifuge tube, centrifuge at 8000r / min for 10min, wash with distilled water 2-3 times, and reserve the yeast sludge for later use.
[0...
Embodiment 2
[0049] (1) Yeast expansion
[0050] Preparation of culture medium (wort): take 2kg of malted malt for saccharification to prepare wort. The saccharification process is as follows: figure 1 shown. Filter after saccharification, remove wheat grains, boil the filtered clear liquid for 60 minutes, and add 0.03% hops during boiling (add 1 / 2 at the beginning of boiling, add 1 / 4 at the time of 30 minutes, add 1 / 4 at the time of 50 minutes) 4). After filtering, the wort was subpackaged and sterilized at 105°C for 10 minutes for later use.
[0051] Take 100 μL of strains Saccharomyces cerevisiae and Saccharomyces cerevisiae stored in glycerol tubes, put them in 10 mL of wort, and culture them at 28°C and 180r / min for 12h, then transfer 10mL of culture liquid to 200mL of fresh wort and culture them at 28°C and 180r / min for 24h, take the culture liquid and centrifuge at 50mL In the tube, centrifuge at 8000r / min for 10min, wash with distilled water 2-3 times, and keep the yeast sludge ...
Embodiment 3
[0068] (1) Yeast expansion
[0069] Preparation of culture medium (wort): take 2kg of malted malt for saccharification to prepare wort. The saccharification process is as follows: figure 1 shown. Filter after saccharification, remove wheat grains, boil the filtered clear liquid for 60 minutes, and add 0.03% hops during boiling (add 1 / 2 at the beginning of boiling, add 1 / 4 at the time of 30 minutes, add 1 / 4 at the time of 50 minutes) 4). After filtering, the wort was subpackaged and sterilized at 105°C for 10 minutes for later use.
[0070] Take 100 μL of strains Saccharomyces cerevisiae and Saccharomyces cerevisiae stored in glycerol tubes, put them in 10 mL of wort, and culture them at 28°C and 180r / min for 12h, then transfer 10mL of culture liquid to 200mL of fresh wort and culture them at 28°C and 180r / min for 24h, take the culture liquid and centrifuge at 50mL In the tube, centrifuge at 8000r / min for 10min, wash with distilled water 2-3 times, and keep the yeast sludge ...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More