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Bacteria culture medium, preparation method thereof and application thereof

A technology of bacterial culture medium and culture medium, applied in the field of microbial detection, can solve problems such as reducing the prevalence of dental caries in the population

Inactive Publication Date: 2019-01-11
河北海棠花医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, most people in our country seek medical treatment only when they have symptoms, and clinical treatment by doctors alone cannot reduce the prevalence of caries in the population as a whole and improve the oral health of the public

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A kind of bacterial culture medium, described culture medium is liquid culture medium, comprises following component in every liter of culture medium: Trypsin 15g, sucrose 80g, sodium chloride 5g, bromocresol blue 1.0g, balance is water.

[0025] The preparation method of the above medium comprises the following steps: weigh the trypsin, add water to dissolve it, add the remaining components, mix evenly, sterilize with saturated steam at 115°C for 30min, aseptically cool to 40°C, and aseptically fill , to obtain the culture medium.

[0026] The above-mentioned medium was used to cultivate oral bacteria. First, a cotton swab was used to gently wipe the tooth surface to take a bacterial sample, inoculated onto the above-mentioned medium, and incubated at a constant temperature of 37°C for 48 hours, and the color change of the medium was observed.

[0027] When the medium is blue, it means that the content of cariogenic bacteria on the tooth surface is low, and the oral co...

Embodiment 2

[0029] A kind of bacterial culture medium, described culture medium is liquid culture medium, comprises following component in every liter of culture medium: trypsin 25g, sucrose 120g, sodium chloride 9g, bromocresol blue 5.5g, surplus is water.

[0030] The preparation method of the above medium comprises the following steps: weigh the trypsin, add water to dissolve it, add the remaining components, mix well, sterilize with saturated steam at 121°C for 15min, aseptically cool to 40°C, and aseptically fill , to obtain the culture medium.

[0031] The above-mentioned medium was used to cultivate oral bacteria. First, a cotton swab was used to gently wipe the tooth surface to take a bacterial sample, inoculated onto the above-mentioned medium, and incubated at a constant temperature of 37°C for 48 hours, and the color change of the medium was observed.

[0032] When the medium is blue, it means that the content of cariogenic bacteria on the tooth surface is low, and the oral con...

Embodiment 3

[0034] A kind of bacterial culture medium, described culture medium is liquid culture medium, comprises following component in every liter of culture medium: trypsin 20g, sucrose 100g, sodium chloride 8g, bromocresol blue 2g, vitamin B0.8mg, vitamin B C350mg, the balance is water.

[0035]The preparation method of the above medium comprises the following steps: weigh the trypsin, add water to dissolve it, add the remaining components, mix well, sterilize with saturated steam at 121°C for 25min, aseptically cool to 40°C, and aseptically fill , to obtain the culture medium.

[0036] The above-mentioned medium was used to cultivate oral bacteria. First, a cotton swab was used to gently wipe the tooth surface to take a bacterial sample, inoculated onto the above-mentioned medium, and incubated at a constant temperature of 37°C for 48 hours, and the color change of the medium was observed.

[0037] When the medium is blue, it means that the content of cariogenic bacteria on the to...

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PUM

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Abstract

The invention provides a bacteria culture medium. The bacteria culture medium comprises nutrients and indicators required for the growth of cariogenic bacteria on the tooth surface, which provides a good environment for the bacteria on the tooth surface to grow, the bacteria on the tooth surface can stably grow on the culture medium, decompose the nutrients in the culture medium to produce acids in the growth process, the higher the concentration of bacteria on the culture medium, the more acid produced, the greater the color change of the culture medium. According to the color change of the culture medium, we can judge the content of cariogenic bacteria on the surface of the sample tooth. The invention judges the content of cariogenic bacteria on the tooth surface by applying the culturemedium to the color change of the culture medium produced by the bacteria on the tooth surface, and is helpful for timely understanding the condition of the cariogenic bacteria on the tooth surface. The bacteria culture medium provided by the invention is simple in composition, reasonable in distribution ratio of each group and convenient in use.

Description

technical field [0001] The invention belongs to the field of microorganism detection, and in particular relates to a bacterial culture medium, its preparation method and application. Background technique [0002] In recent years, although people's overall health and oral health have been continuously improved, oral diseases, especially dental caries, are still very common health problems. Take children as an example: Children may suffer from dental caries from about 1 year old. As they grow older, the caries rate of children increases linearly and reaches a peak at the age of 7 or 8. The results of the third national oral health epidemiological survey in 2005 showed that the deciduous tooth caries rate of 5-year-old children in my country was as high as 66%, with 3.5 carious teeth per capita, ranking the highest in the world. The caries rate of permanent teeth of 12-year-old children reaches 28.9%, and more than 70% of adults suffer from caries, most of which have not been ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12Q1/04C12R1/46C12R1/01C12R1/225
CPCC12N1/20C12Q1/045G01N2333/315G01N2333/335G01N2333/355
Inventor 杨子中刘海洋李彪朱丽刚朱智明郝秋星张凌宇陈冲蔺杰
Owner 河北海棠花医疗器械有限公司