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Method for rapid seedling formation of dendrobium officinale

A technology of Dendrobium officinale and stem segments is applied in the field of rapid seedling growth of Dendrobium officinale, can solve the problems of low seedling rate, low reproduction coefficient, long reproduction cycle, etc., and achieves the effects of saving cost, ensuring survival rate and shortening cultivation cycle

Inactive Publication Date: 2019-01-15
NANJING LONGYUAN ECOLOGICAL AGRI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional asexual reproduction method has a low reproduction coefficient. The cultivation of protocorms undergoes protocorm induction, proliferation, differentiation, rooting, strong seedlings, and seedling hardening steps. The reproductive cycle is long and the seedling rate is low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0113] A method for rapidly growing seedlings of Dendrobium officinale, the cultivation steps comprising the following steps:

[0114] 1) Collection and disinfection

[0115] Cut the young stems of 2-5cm long from Dendrobium officinale plants, wash them under running water, gently scrub them with a brush dipped in a little washing powder aqueous solution, and wash them thoroughly with tap water, place the stems on an ultra-clean workbench After disinfecting in 75% alcohol for 30 seconds, soak in 0.1% HgCl2 aqueous solution for 8-10 minutes, and rinse with sterile water for 5-6 times;

[0116] 2) Induction culture

[0117] Induction culture: cut off the young stems of Dendrobium officinale with a scalpel, and inoculate them in the induction medium for cultivation; after 15 to 20 days, the explants begin to germinate, the stems expand, and the cut surface and top tissue form loose tissues; continue After culturing for 20 days, transfer it to the micro-placement chamber, the cu...

Embodiment 2

[0125] A method for rapidly growing seedlings of Dendrobium officinale, the cultivation steps comprising the following steps:

[0126] 1) Collection and disinfection

[0127] Cut the young stems of 2-5cm long from Dendrobium officinale plants, wash them under running water, gently scrub them with a brush dipped in a little washing powder aqueous solution, and wash them thoroughly with tap water, place the stems on an ultra-clean workbench After disinfecting in 75% alcohol for 30 seconds, soak in 0.1% HgCl2 aqueous solution for 8-10 minutes, and rinse with sterile water for 5-6 times;

[0128] 2) Induction culture

[0129] Induction culture: cut off the young stems of Dendrobium officinale with a scalpel, and inoculate them in the induction medium for cultivation; after 15 to 20 days, the explants begin to germinate, the stems expand, and the cut surface and top tissue form loose tissues; continue After culturing for 20 days, transfer it to the micro-placement chamber, the cu...

Embodiment 3

[0137] A method for rapidly growing seedlings of Dendrobium officinale, the cultivation steps comprising the following steps:

[0138] 1) Collection and disinfection

[0139] Cut the young stems of 2-5cm long from Dendrobium officinale plants, wash them under running water, gently scrub them with a brush dipped in a little washing powder aqueous solution, and wash them thoroughly with tap water, place the stems on an ultra-clean workbench After disinfecting in 75% alcohol for 30 seconds, soak in 0.1% HgCl2 aqueous solution for 8-10 minutes, and rinse with sterile water for 5-6 times;

[0140] 2) Induction culture

[0141]Induction culture: cut off the young stems of Dendrobium officinale with a scalpel, and inoculate them in the induction medium for cultivation; after 15 to 20 days, the explants begin to germinate, the stems expand, and the cut surface and top tissue form loose tissues; continue After culturing for 20 days, transfer it to the micro-placement chamber, the cut...

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Abstract

The invention discloses a method for rapid seedling formation of dendrobium officinale. The method comprises the steps successively: 1) taking materials and disinfecting; 2) carrying out induction culture; 3) carrying out proliferation and differentiation culture, transferring protocorms formed from induction into a proliferation and differentiation culture medium, at the same time, allowing a part of protocorms to begin to differentiate into bud points and grow out 1-2 leaflets, and allowing proliferation and bud differentiation of the protocorms to be carried out simultaneously, wherein thetemperature is 26 DEG C, the illumination intensity is 2200-2500 lx, and the illumination time is 13 h / d; 4) carrying out rooting culture; and 5) carrying out transitional management of bottled seedlings. Through cultivation in all the steps, the seedling refining link is omitted, the cultivation period is greatly shortened, the cost is saved, and the survival rate of the seedlings is ensured.

Description

technical field [0001] The invention belongs to the field of dendrobium cultivation, and in particular relates to a method for rapidly growing seedlings of dendrobium candidum. Background technique [0002] Dendrobium candidum, commonly known as Tiepi Fengdou, is a perennial herb of the genus Dendrobium in the family Orchidaceae. [0003] It is a precious traditional Chinese medicine with important medicinal value. It has the effects of nourishing yin and clearing heat, promoting body fluid and benefiting stomach, relieving cough and moistening lung, and has considerable market development value. In recent years, due to people's excessive excavation of it, its wild resources have decreased sharply, and it has become increasingly endangered. It has been listed as a national key protected plant. Because Dendrobium candidum has high requirements on the ecological environment, the reproductive rate of Dendrobium candidum is low under natural conditions and the growth rate is sl...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 邵丽丽刘琴
Owner NANJING LONGYUAN ECOLOGICAL AGRI CO LTD