Unlock instant, AI-driven research and patent intelligence for your innovation.

Expression of porcine circovirus type 3 cap protein by recombinant baculovirus and its construction method and primers

A technology of recombinant baculovirus and porcine circovirus, applied in the direction of viruses, viral peptides, viruses/phages, etc., to achieve the effect of good immune reactivity

Active Publication Date: 2022-05-27
YANGZHOU UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports on PCV3 vaccines, and the expression of PCV3 Cap protein by constructing recombinant baculovirus can lay the foundation for the development of PCV3 subunit vaccines

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Expression of porcine circovirus type 3 cap protein by recombinant baculovirus and its construction method and primers
  • Expression of porcine circovirus type 3 cap protein by recombinant baculovirus and its construction method and primers
  • Expression of porcine circovirus type 3 cap protein by recombinant baculovirus and its construction method and primers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0039] 1. Construction of recombinant baculovirus rBac-Cap expressing porcine circovirus type 3 Cap gene:

[0040] The primer sequences designed for amplification are as follows:

[0041] Table 1 Required primers and restriction sites

[0042]

[0043]

[0044] Note: The underlined part is each restriction site

[0045] 1. Construct pSK-sPCV3 containing the whole genome of PCV3:

[0046] Using the extracted PCV3 DNA as a template, the whole PCV3 genome was amplified with primers Xho I-3F and EcoR I-3R; the PCV3 whole genome gel was recovered and the pBluescript SK(+) vector preserved in the laboratory was simultaneously used with Xho I and EcoRI double digestion, the two digestion products were purified and recovered, and then connected at a molar ratio of 3:1, and finally the recombinant plasmid pSK-sPCV3 was obtained. The recombinant plasmid was identified by double digestion with Xho I and EcoR I, and the digestion product was electrophoresed on a 0.7% agarose gel ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention belongs to the technical field of vaccine manufacture. The invention discloses a recombinant baculovirus expressing porcine circovirus type 3 Cap protein. The invention also discloses primers for constructing recombinant baculovirus expressing porcine circovirus type 3 Cap protein. The present invention also discloses a construction method for expressing porcine circovirus type 3 Cap protein by recombinant baculovirus, comprising the following steps: 1. Construction of pSK-sPCV3 containing the whole PCV3 genome; 2. Construction of transfer vector; 3. Shuttle Construction of plasmid; 4. Obtaining of recombinant baculovirus; transfecting Sf9 cells with positive shuttle plasmid Bacmid-Cap to obtain recombinant baculovirus rBac-Cap expressing porcine circovirus type 3 Cap gene. The Cap protein expressed in the present invention has good biological activity, and it is found to have good immunogenicity in immunized mice.

Description

technical field [0001] The present invention relates to a method for constructing a recombinant baculovirus expressing porcine circovirus type 3 (PCV3) Cap gene, which is used for making vaccines. Background technique [0002] Porcine circovirus disease (PCVD) is one of the important diseases currently endangering the swine industry, of which PCV2 is the main pathogenic factor causing this type of disease. In 2016, scholars such as Phan reported a novel porcine circovirus PCV3, which can cause porcine dermatitis and nephrotic syndrome (PDNS), porcine reproductive disorders, respiratory diseases, and digestive system inflammation [Phan TG, Giannitti F, Rossow S]. , et al.Detection of a novel circovirus PCV3 in pigs withcardiac and multi-systemic inflammation[J].Virology Journal,2016,13(1):184.]. Through the genome-wide genetic evolution analysis of PCV3, it was found that PCV3 is in a different evolutionary branch from the other two genotypes of PCV1 and PCV2, and there are ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/01C12N15/11C12N15/866C12N15/34C12R1/93
CPCC12N7/00C12N15/86C07K14/005C12N2710/14143C12N2710/14121C12N2800/105C12N2750/10022
Inventor 高崧程清如高清清王小波郇长超刘秀梵
Owner YANGZHOU UNIV