A kind of heavy metal polluted soil bioremediation agent and its repair method

A bioremediation agent and technology for polluted soil, applied in the field of heavy metal contaminated soil bioremediation agent and its restoration, can solve the problems that cannot be applied outdoors in a large area, heavy metal soil cannot be repaired, microorganisms are difficult to adapt to the natural environment, etc.

Active Publication Date: 2021-05-04
万物生(深圳)生物科技控股有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the above-mentioned scheme has the following defects: the microorganisms in the above-mentioned scheme are all cultivated under the condition of laboratory medium, so they cannot be applied outdoors on a large scale. If they are directly applied outdoors, the living environment of the microorganisms will change, which may cause the microorganisms to be difficult to adapt. The natural environment affects the survival rate of microorganisms, so it cannot effectively repair the heavy metal soil

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  • A kind of heavy metal polluted soil bioremediation agent and its repair method
  • A kind of heavy metal polluted soil bioremediation agent and its repair method

Examples

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Effect test

Embodiment 1

[0024] A bioremediation agent for soil heavy metal pollution is prepared from the following raw materials according to the percentage of the total mass of raw materials: 5% of biological bacteria stock solution, 1% of antibacterial agent, and the rest is nutrient solution.

[0025] The microorganisms in the biological bacteria stock solution are Rhodobacter sphaericus, and the effective number of viable bacteria in the biological bacteria stock solution is 3 billion / g.

[0026] The components in the nutrient solution are calculated by weight percentage: 64% of distilled water, 6% of lactic acid, 5% of sodium chloride, 10% of peptone, 5% of ammonium phosphate, 8% of glucose and 2% of ascorbic acid.

[0027] Described antibacterial agent is wormwood essential oil.

[0028] At the same time, the invention also discloses a bioremediation method for heavy metal contaminated soil, the specific operation steps are as follows:

[0029] S1: Microbial culture, weigh heavy metal contami...

Embodiment 2

[0034] A bioremediation agent for soil heavy metal pollution is prepared from the following raw materials according to the percentage of the total mass of raw materials: 7% of biological bacteria stock solution, 2% of antibacterial agent, and the rest is nutrient solution.

[0035] The microorganisms in the biological bacteria stock solution are Rhodobacter sphaericus, and the effective number of viable bacteria in the biological bacteria stock solution is 4 billion / g.

[0036] The components in the nutrient solution are calculated by weight percentage: 64% of distilled water, 7% of lactic acid, 5% of sodium chloride, 11% of peptone, 6% of ammonium phosphate, 6% of glucose and 1% of ascorbic acid.

[0037] Described antibacterial agent is lavender essential oil.

[0038] At the same time, the invention also discloses a bioremediation method for heavy metal contaminated soil, the specific operation steps are as follows:

[0039] S1: Microbial culture, weighing heavy metal cont...

Embodiment 3

[0044] A bioremediation agent for soil heavy metal pollution is prepared from the following raw materials according to the percentage of the total mass of raw materials: 8% of biological bacteria stock solution, 2% of antibacterial agent, and the rest is nutrient solution.

[0045] The microorganisms in the biological bacteria stock solution are Rhodobacter sphaericus, and the effective number of viable bacteria in the biological bacteria stock solution is 5 billion / g.

[0046]The components in the nutrient solution are calculated by weight percentage: 54% of distilled water, 10% of lactic acid, 6% of sodium chloride, 15% of peptone, 7% of ammonium phosphate, 7% of glucose and 1% of ascorbic acid.

[0047] Described antibacterial agent is clary sage essential oil.

[0048] At the same time, the invention also discloses a bioremediation method for heavy metal contaminated soil, the specific operation steps are as follows:

[0049] S1: Microbial culture, weighing the heavy meta...

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Abstract

The invention discloses a bioremediation agent for soil heavy metal pollution and a restoration method thereof. The bioremediation agent is made of the following raw materials according to the percentage of the total mass of raw materials: biological bacteria stock solution 5-8%, bacteriostatic agent 1 ‑2%, the rest is nutrient solution. The present invention uses microorganisms to treat heavy metal-contaminated soil, which avoids secondary pollution to the soil, does not affect soil fertility, and is beneficial to the subsequent planting of crops. By pre-cultivating microorganisms in heavy metal soil, it is possible to obtain suitable for this kind of soil. Microorganisms, by adjusting the pH of the soil, help the microorganisms to adapt to the soil environment in the subsequent treatment process and improve the survival rate of the microorganisms, thereby ensuring the remediation efficiency of heavy metal-contaminated soil.

Description

technical field [0001] The invention belongs to the technical field of soil remediation, and more specifically relates to a bioremediation agent for heavy metal polluted soil and a remediation method thereof. Background technique [0002] Soil is a natural resource for human beings to survive, and it is also the material basis for human beings to obtain food and other renewable resources. With the development of industry and the modernization of agricultural development, soil heavy metal pollution has become a major soil problem. Soil heavy metal pollution has pollutants that move in the soil It has the characteristics of poor performance, long residence time, and cannot be degraded by microorganisms, and can eventually affect human health through media such as water and plants. [0003] For example, the patent application number CN201110122739.6 discloses a microbial repair method for heavy metal-contaminated soil, which specifically includes: Step 1: Separation of Rhodobac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B09C1/10
CPCB09C1/10B09C2101/00
Inventor 刘永飞马敏戚晓楠
Owner 万物生(深圳)生物科技控股有限公司
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