Probiotic preparation and preparing method and application thereof

A technology of probiotic preparations and live bacteria, applied in the field of probiotic preparations and its preparation, can solve problems such as excrement and residual bait pollution, damage to the micro-ecological balance of aquaculture water, and water quality deterioration

Active Publication Date: 2019-01-22
XIAMEN HUIYING ANIMAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, a series of problems have also appeared in the process of aquaculture, for example, due to excessive feeding and fertilization, harmful microorganisms and harmful algae multiply rapidly, as well as excrement and residual bait pollution, ammonia nitrogen and nitrite content increase, damage The micro-e

Method used

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  • Probiotic preparation and preparing method and application thereof
  • Probiotic preparation and preparing method and application thereof

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Experimental program
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Embodiment approach

[0027] According to a preferred embodiment of the present invention, the method for liquid fermentation comprises the following steps:

[0028] (1) Activation of the original strain: Bacillus subtilis and Bacillus natto were respectively inoculated into sterilized slant medium I for aerobic culture until colonies grew, and frozen preserved Lactobacillus plantarum, Lactobacillus acidophilus and Lactobacillus casei was inoculated into sterilized slant medium II for aerobic culture until colonies grew out, and Candida utilis and Saccharomyces cerevisiae were respectively inoculated into sterilized slant medium III for aerobic culture until they grew out. Bacterial colonies, inoculating Rhodopseudomonas palustris into sterilized slant medium IV and anaerobic culture until colonies grow to obtain respective activated slant strains;

[0029] The slant medium I contains beef extract 1-5g, peptone 5-15g, sodium chloride 2-8g, agar 10-30g and distilled water 1L and the pH value is 7.2-...

Embodiment 1

[0044] This embodiment is used to illustrate the probiotic preparation provided by the present invention and its preparation method.

[0045](1) Activation of the original strains: activate the streaked slopes of various strains stored at -18°C, wherein Bacillus subtilis and Bacillus natto were cultured aerobically at 37°C for 18 hours with slope medium I respectively, wherein, The specific formula of slant medium I is as follows: beef extract 3g, peptone 10g, sodium chloride 5g, agar 20g and distilled water 1L, pH value 7.2, sterilized at 121°C for 20min. Lactobacillus plantarum, Lactobacillus acidophilus and Lactobacillus casei were anaerobically statically cultured at 37° C. for 24 h with slant medium II (MRS medium culture) respectively, wherein the specific formula of slant medium II was as follows: 10.0 g of peptone , beef powder 5.0g, yeast powder 4.0g, glucose 20.0g, Tween 80 1.0mL, dipotassium hydrogen phosphate 2.0g, sodium acetate 5.0g, triammonium citrate 2.0g, mag...

Embodiment 2

[0051] This embodiment is used to illustrate the probiotic preparation provided by the present invention and its preparation method.

[0052] (1) Activation of the original strains: activate the streaked slopes of various strains stored at -18°C, wherein Bacillus subtilis and Bacillus natto were cultured aerobically at 37°C for 18 hours with slope medium I respectively, wherein, The specific formula of slant medium I is as follows: beef extract 1g, peptone 5g, sodium chloride 2g, agar 10g and distilled water 1L, pH value 7.5, sterilized at 121°C for 20min. Lactobacillus plantarum, Lactobacillus acidophilus and Lactobacillus casei were anaerobically statically cultured at 37° C. for 24 h with slant medium II (MRS medium culture) respectively, wherein the specific formula of slant medium II was as follows: 5.0 g of peptone , beef powder 2.0g, yeast powder 2.0g, glucose 10.0g, Tween 80 0.5mL, dipotassium hydrogen phosphate 1.0g, sodium acetate 2.0g, triammonium citrate 1.0g, magn...

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Abstract

The invention relates to the field of aquaculture and discloses a probiotic preparation and a preparing method and application thereof. The probiotic preparation is prepared from bacillus, lactic acidbacteria, saccharomycetes and photosynthetic bacteria through liquid fermentation. The bacillus consists of bacillus subtilis and bacillus natto. The lactic acid bacteria consist of lactobacillus plantarum, lactobacillus acidophilus and lactobacillus casei. The saccharomycetes consist of candida utilis and saccharomyces cerevisiae. Rhodopseudomonas palustris severs as the photosynthetic bacteria.According to the probiotic preparation, ammonia nitrogen and nitrite in an aquaculture water body can be effectively degraded, water body organic matter is decomposed, the pH value of the water bodyis adjusted, the content of dissolved oxygen of the water body is increased, and meanwhile, harmful pathogens in the aquaculture water body can further be inhibited; and in addition, the probiotic preparation can further improve the micro-ecological environment of intestinal tracts of aquatic animals, adjust balance of intestinal flora and inhibit the growth of harmful bacteria, thus nutrient absorption is increased advantageously, and the feed utilization rate is increased.

Description

technical field [0001] The invention belongs to the field of aquaculture, and in particular relates to a probiotic preparation and a preparation method and application thereof. Background technique [0002] Aquatic animal breeding is mainly engaged in aquatic economic animal breeding by using aquaculture technology and facilities in accordance with the biological characteristics of aquaculture animals and the requirements for water environment conditions, using fresh water or sea water that can be aquacultured. In recent years, with the continuous improvement of our country's living standards and economic conditions, the supply of aquatic products has also increased significantly, and aquaculture has attracted more and more attention. However, a series of problems have also appeared in the process of aquaculture, for example, due to excessive feeding and fertilization, harmful microorganisms and harmful algae multiply rapidly, as well as excrement and residual bait pollution...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16A23K50/80A23K10/18C02F3/34C12R1/125C12R1/07C12R1/25C12R1/23C12R1/245C12R1/865C12R1/72
CPCA23K10/18A23K50/80C12N1/16C12N1/20C02F3/34A23V2400/125A23V2400/113A23V2400/169
Inventor 林松泉庄若飞邹文政乔欣君黄榕
Owner XIAMEN HUIYING ANIMAL TECH
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