A method for preparing n-acetylneuraminic acid by converting n-acetylneuraminic acid hydrate with an organic solvent
A technology of acetylneuraminic acid and organic solvent, which is applied in the field of chemical engineering and achieves the effects of satisfying market demand, high product purity and low cost
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Embodiment 1
[0021] In this implementation, N-acetylneuraminic acid is prepared from N-acetylneuraminic acid hydrate using an organic solvent as follows:
[0022] 1. Select N-acetylneuraminic acid dihydrate as raw material, and the purity of N-acetylneuraminic acid dihydrate is 98.2%.
[0023] 2. Weigh 10g of the above-mentioned N-acetylneuraminic acid dihydrate, and weigh 60g of ethanol according to the weight ratio of N-acetylneuraminic acid hydrate to ethanol is 6:1;
[0024] 3. Pour the above ethanol into the weighed N-acetylneuraminic acid dihydrate container, and put it into a 30°C water bath for stirring, and keep stirring until all the hydrates are converted into N-acetylneuraminic acid until.
[0025] 4. Filter the converted N-acetylneuraminic acid, filter with filter paper, and then quickly rinse twice with pure water.
[0026] 5. The above washed N-acetylneuraminic acid was put into an oven for drying at 80° C., and then detected by high performance liquid chromatography, the ...
Embodiment 2
[0028] In this implementation, N-acetylneuraminic acid is prepared by dehydrating N-acetylneuraminic acid hydrate with an organic solvent as follows:
[0029] 1. Select N-acetylneuraminic acid dihydrate as raw material, and the purity of N-acetylneuraminic acid dihydrate is 98.5%.
[0030] 2. Weigh 10g of the above-mentioned N-acetylneuraminic acid dihydrate, and at the same time weigh 80g of ethanol according to the ratio of N-acetylneuraminic acid hydrate to ethanol 8:1;
[0031] 3. Pour the above ethanol into the weighed container of N-acetylneuraminic acid dihydrate, and put it into a 60°C water bath for stirring until all the hydrates are converted into N-acetylneuraminic acid until.
[0032] 4. Filter the converted N-acetylneuraminic acid, filter with filter paper, and then quickly rinse twice with pure water.
[0033] 5. The above washed N-acetylneuraminic acid was put into an oven for drying at 60° C., and then detected by high performance liquid chromatography, the ...
Embodiment 3
[0035] This implementation follows the steps of Example 2, except that the ratio of N-acetylneuraminic acid hydrate to ethanol in step 2 is 11:1, the others are the same as the steps of Example 2, and the purity of the finally obtained N-acetylneuraminic acid is 95%. %.
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