Unlock instant, AI-driven research and patent intelligence for your innovation.

Extraction method of soybean water-soluble protein

A technology of water-soluble protein and extraction method, which is applied in the field of food safety and quality inspection

Inactive Publication Date: 2019-01-25
NANJING UNIV OF FINANCE & ECONOMICS
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Kjeldahl method is classic and accurate, and is suitable for the determination of protein in food, but there are few reports on improving the extraction rate of water-soluble protein, so the research in this area has great prospects

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Extraction method of soybean water-soluble protein
  • Extraction method of soybean water-soluble protein
  • Extraction method of soybean water-soluble protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The soybeans are ground by a vertical cyclone mill and mixed for later use. Weigh 1.000 g (accurate to 0.001 g) of the sample into a 50 ml centrifuge tube, add 35 ml of distilled water, and shake to disperse the sample evenly. Place the centrifuge tube in a constant temperature water bath at 30°C and shake for 90 minutes, and then centrifuge at a speed of 3000 r / min for 10 minutes. After centrifugation, take 10ml of sample in the digestion tube, and add 12ml of concentrated sulfuric acid and catalyst to the digestion tube at the same time. After digestion, the automatic Kjeldahl nitrogen analyzer was used to measure and record the data.

Embodiment 2

[0033] The soybeans are ground by a vertical cyclone mill and mixed for later use. Weigh 1.000 g (accurate to 0.001 g) of the sample into a 50 ml centrifuge tube, add 40 ml of distilled water, and shake to disperse the sample evenly. Place the centrifuge tube in a constant temperature water bath at 35°C and shake for 120 minutes, and then centrifuge at a speed of 3000 r / min for 10 minutes. After centrifugation, take 10ml of sample in the digestion tube, and add 12ml of concentrated sulfuric acid and catalyst to the digestion tube at the same time. After digestion, the automatic Kjeldahl nitrogen analyzer was used to measure and record the data.

Embodiment 3

[0035] The soybeans are ground by a vertical cyclone mill and mixed for later use. Weigh 1.000 g (accurate to 0.001 g) of the sample into a 50 ml centrifuge tube, add 50 ml of distilled water, and shake to disperse the sample evenly. Place the centrifuge tube in a constant temperature water bath at 40°C for 150 min, then centrifuge at 3000 r / min for 10 min. After centrifugation, take 10ml of sample in the digestion tube, and add 12ml of concentrated sulfuric acid and catalyst to the digestion tube at the same time. After digestion, the automatic Kjeldahl nitrogen analyzer was used to measure and record the data.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an extraction method of soybean water-soluble protein. Soybeans are used as raw materials and an optimal extraction technology of the soybean water-soluble protein is researched; meanwhile, compared with an existing national and industrial standard preparation method, the extraction method has the advantage of further improving the extraction efficiency of the water-solubleprotein. An experiment adopts a single-factor test to compare influences, caused by a material-liquid ratio, digestion temperature and extraction time, on the extraction rate and stability of the soybean water-soluble protein. An optimal combination of the digestion temperature, the extraction time and the material-liquid ratio is obtained through orthogonal experiment optimization; the optimizedextraction and detection rate of the water-soluble protein is improved by 3.67 percent. Compared with other detection manners, the extraction method has the characteristics of rapidness, convenience,high efficiency and stability and has important meaning on the safety quality of grains.

Description

technical field [0001] The invention optimizes the soybean water-soluble protein extraction process based on relevant industry standards, and belongs to the field of food safety quality detection. Background technique [0002] Soybeans are often used in my country to make a variety of soy foods, or to extract soybean oil, brew soy sauce, or to make protein. Soybean is an excellent source of protein, containing all essential amino acids, and its processing function, nutritional and flavor properties play an important role in the food industry. Among all grains and oils, soybeans have the highest protein content, accounting for about 30% to 50% of the total mass. Usually, more than 90% of the protein in newly harvested soybeans can be dissolved in water. This part of the protein is called water-soluble protein. At present, proteins that can dissolve in water are widely used in production and processing, while non-water-soluble proteins are left in bean dregs. Therefore, the e...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/40G01N31/16
CPCG01N1/286G01N1/4077G01N31/16G01N2001/2866
Inventor 袁建郝怡宁邢常瑞初晨露陈尚兵
Owner NANJING UNIV OF FINANCE & ECONOMICS