Method for preparing sialoglycopeptide from tuna eggs

A technology of sialic acid sugar and tuna, which is applied in the field of food processing to achieve the effect of high extraction rate and improved safety

Active Publication Date: 2019-01-29
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on tuna egg sialoglycopeptide

Method used

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  • Method for preparing sialoglycopeptide from tuna eggs
  • Method for preparing sialoglycopeptide from tuna eggs
  • Method for preparing sialoglycopeptide from tuna eggs

Examples

Experimental program
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Effect test

Embodiment 1

[0043] This implementation case shows a method for preparing sialic acid glycopeptide from tuna roe according to the following steps:

[0044]The frozen tuna roe was taken out and thawed at 4°C, homogenized by a homogenizer, frozen at -20°C for 12 hours, and freeze-dried by a freeze dryer to obtain tuna roe powder. Add 10 times the volume of absolute ethanol (1 / 10, w / v) to the freeze-dried fish egg powder and stir for 10 h to degrease, during which the absolute ethanol is replaced every 3 h. After degreasing, ethanol is removed by double-layer fat filtration, and the filter residue is air-dried in a cold air dryer for 6 hours to obtain defatted tuna roe powder. Take defatted fish egg powder and add deionized water to extract sialic acid glycopeptide (1 / 20, w / v) according to 20 times of its weight to volume ratio. The extraction temperature is 4° C., and the extraction time is 12 hours. During the period, ultrasonic treatment was performed every hour to assist in the extractio...

Embodiment 2

[0046] This implementation case shows a method for preparing sialic acid glycopeptides from tuna eggs according to the following steps: take out the frozen tuna eggs and thaw them at 4°C, homogenize them with a homogenizer, and freeze them at -20°C for 12 hours. The tuna roe powder is obtained by freeze-drying with a freeze dryer. Add 13 times the volume of absolute ethanol (1 / 12, w / v) to the freeze-dried fish egg powder and stir for 10 h to degrease, during which the absolute ethanol is replaced every 3 h. After degreasing, ethanol was removed by double-layer fat filtration, and the filter residue was air-dried in a cold air dryer for 8 hours to obtain defatted tuna roe powder. Take defatted fish egg powder and add deionized water according to its weight-to-volume ratio 25 times to extract sialic acid glycopeptide (1 / 25, w / v). The extraction temperature is 8° C. and the extraction time is 14 hours. During the period, ultrasonic treatment was performed every hour to assist in...

Embodiment 3

[0048] This implementation case shows a method for preparing sialic acid glycopeptide from tuna roe according to the following steps:

[0049] The frozen tuna roe was taken out and thawed at 4°C, homogenized by a homogenizer, frozen at -20°C for 12 hours, and freeze-dried by a freeze dryer to obtain tuna roe powder. Add 15 times the volume of absolute ethanol (1 / 15, w / v) to the freeze-dried fish egg powder and stir for 10 h to degrease, during which the absolute ethanol is replaced every 3 h. After degreasing, ethanol is removed by double-layer fat filtration, and the filter residue is air-dried in a cold air drier for 10 hours to obtain defatted tuna roe powder. Take defatted fish egg powder and add deionized water according to 30 times of its weight-to-volume ratio to extract sialic acid glycopeptide (1 / 30, w / v). The extraction temperature is 10° C., and the extraction time is 16 hours. During the period, ultrasonic treatment was performed every hour to assist in the extrac...

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Abstract

The invention discloses a method for preparing sialoglycopeptide from tuna eggs. The method makes up the blank of tuna processing industry of our country in the aspect of sialoglycopeptide. The methodcomprises the steps of subjecting defatted fish eggs to an ultrasonic assisted water extraction method so as to obtain a tuna-egg sialoglycopeptide crude product, carrying out dextran-gel G-50 columnseparation and activated-charcoal adsorption, and then, carrying out eluting by using a methanol-water system, thereby obtaining tuna-egg sialoglycopeptide purified product. The preparation method issimple and convenient, the extraction rate is relatively high, and the safety of the product is improved.

Description

technical field [0001] The invention relates to the technical field of food processing, in particular to a method for preparing sialic acid glycopeptides from tuna eggs. Background technique [0002] Sialic acid glycopeptide is a complex N-glycosylated glycopeptide, which can only be isolated from natural products. Sialoglycopeptide is a humanized glycopeptide, which can regulate the permeability of glomerulus and inhibit the growth of osteoclasts. Highly active sialoglycopeptides mainly exist in sexually mature animal egg cells. The international methods for separating and purifying sialoglycopeptides are mainly based on the method reported by Akira Seko in Japan in 1997. This method needs to go through two Sephadex separations, one DEAE ion exchange column and one CM-SephadexC separation, the process is very complicated and the cycle is long. A domestic patent uses a dextran gel for separation. After adsorption by activated carbon, it uses acetonitrile elution to obtain...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K9/00C07K1/14C07K1/16
CPCC07K9/00
Inventor 胡世伟李世杰姜维刘宇
Owner ZHEJIANG OCEAN UNIV
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