Dwarf hydrangea macrophylla tissue culture method

A tissue culture and hydrangea technology, applied in the field of plant cultivation and reproduction, achieves the effects of broad market prospects, high survival rate, and shortening of seedling time.

Pending Publication Date: 2019-02-01
HANGZHOU LANDSCAPING +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, domestic research on tissue culture of hydrangeas is mostly

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Object: Dwarf big-leaf hydrangea "Wanhuajing"

[0022] Step 1) Put the container seedlings into the greenhouse in March, apply 5g of compound fertilizer with a nitrogen:phosphorus:potassium ratio of 28:5:5, water once a day in the morning and evening, and spray 600 times of carbendazim solution every 7 days on the whole plant After 40 days, collect new branches; remove the leaves of the collected new branches, wipe them clean with 75% alcohol cotton balls, put them into a mixed solution of detergent and 10% sodium hypochlorite, stir them on a magnetic stirrer for 30 minutes, and then rinse them with tap water for 1 hour spare;

[0023] Step 2) On the ultra-clean workbench, soak in 75% alcohol for 10 seconds, after pouring off the alcohol, soak in 0.1% mercuric acid solution for 10 minutes, shake constantly during this period, after pouring out the mercuric solution, rinse with sterile water 5 times for later use;

[0024] Step 3) Cut into small sections with a sterile ...

Embodiment 2

[0029] Target: Dwarf big-leaf hydrangea "Hanatemari"

[0030] The new shoots collected after 30 days in step 1) are put into detergent and 10% sodium hypochlorite mixed solution, stirred on a magnetic stirrer for 20 min, and then rinsed with tap water for 0.5 h for later use; the starting medium formula in step 3) is B 5 Basic medium, add 1.0mg·L -1 6-Benzylaminopurine, 0.1mg·L -1 Naphthaleneacetic acid, 0.7% carrageenan and 2.0% sucrose, the pH is adjusted to 5.4; step 4) in the proliferation medium formula is B 5 Basic medium, add 1.5mg·L -1 6-Benzylaminopurine, 0.1mg·L -1 Naphthaleneacetic acid, 0.7% carrageenan and 1.0% sucrose, the pH is adjusted to 5.4; Step 6) carries out rooting outside the bottle in July; The twig base is at 600mg L -1 Dip in naphthaleneacetic acid at a medium speed for 5s; the average temperature of the full-light spraying greenhouse is 35°C, adjust the spray interval and duration to keep the relative humidity at 90%, and spray 500 times of carbe...

Embodiment 3

[0032] Target: Dwarf big-leaf hydrangea "Miss Saori"

[0033] The new shoots collected after 35 days in step 1) are put into detergent and 10% sodium hypochlorite mixed solution, stirred on a magnetic stirrer for 25 minutes, and then rinsed with tap water for 0.6 hours for later use; in step 3), the starting medium formula is B 5 Basic medium, add 1.0mg·L -1 6-Benzylaminopurine, 0.1mg·L -1Naphthaleneacetic acid, 0.7% carrageenan and 2.0% sucrose, the pH is adjusted to 5.4; step 4) in the proliferation medium formula is B 5 Basic medium, add 2.0mg·L -1 6-Benzylaminopurine, 0.1mg·L -1 Naphthaleneacetic acid, 0.7% carrageenan and 1.0% sucrose, the pH is adjusted to 5.4; Step 6) carries out rooting outside the bottle in December; The twig base is at 700mg L -1 Dip in naphthaleneacetic acid at a medium speed for 5 seconds; the average temperature of the full-light spraying greenhouse is 15°C, adjust the spray interval and duration to keep the relative humidity at 70%, and spray...

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Abstract

The invention provides a dwarf hydrangea macrophylla tissue culture method, which comprises the following steps of: 1) selecting healthy and robustly growing and disease-free dwarf hydrangea macrophylla seedlings, scissoring newly growing branches, removing leaves, retaining parts of petioles, and performing cleaning for standby use; 2) conducting immersion disinfection on the branches with an alcohol solution and a mercuric chloride solution in order on a super clean bench, and rinsing the branches clean; 3) cutting the branches into small pieces, inoculating the small pieces of branches intoa medium, and conducting culture in a culture room; 4) after culturing for a period of time, sprouting axillary buds, cutting off the sprout tender branches, and inoculating the branches into a proliferation medium for culture; 5) after culture for a period of time, forming several adventitious buds at the root, when the buds grow to a certain height, conducting acclimation in a greenhouse; and 6) cleaning the seedlings, cutting adventitious buds, and performing ex vitro rooting. The method provided by the invention has the characteristics of high survival rate, short propagation period, a multiplication coefficient up to 6.2, and a rooting rate up to 93%, completes rooting and seedling hardening in one step, shortens the seedling raising time by 1-2 months, can realize anniversary generation of dwarf hydrangea macrophylla, and has broad market prospects.

Description

technical field [0001] The invention belongs to the technical field of plant breeding and reproduction, and in particular relates to a tissue culture method for dwarf hydrangea large leaves. Background technique [0002] Dwarf hydrangea is a general term for a class of cultivars of macrophylla of the genus Hydrangea in the family Saxifragaceae. The plant type is short and compact, and the plant height is 30-40cm; -5cm, which is 1 / 5 of the ordinary big-leaf hydrangea; the inflorescence is large and beautiful, with rich colors, including red, pink, blue, purple, white and green; it is a terminal corymb-shaped cyme Inflorescences are further divided into flat inflorescences and spherical inflorescences according to the number of decorative flowers and the way they grow; the shape, position and number of sepals of decorative flowers also vary in abundance. The dwarf type big-leaf hydrangea likes half shade, is not light-resistant, and likes fertile, moist, and well-drained soil...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 孟雨冉邱帅秦俊张宪权张俊林郭娟樊靖胡玉春
Owner HANGZHOU LANDSCAPING
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