Probiotics-containing care lotion for external use

A technology of probiotics and lotion, applied in the field of microorganisms, can solve problems such as skin irritation, unfavorable promotion and use, and toxic effects of lactobacilli, and achieve the effect of good synergistic performance and good application prospects

Pending Publication Date: 2019-02-01
杨丽华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

② Certain spermicidal contraceptive creams have toxic effects on Lactobacillus
[0004] At present, there are few probiotic care solutions used in gynecology; Chinese patent "2016100929816, an ecological gynecological external pharmaceutical composition and its preparation method and application" discloses a gynecological external composition fermented by probiotics, the composition It is the compatibility of lactic acid bacteria fermentation product and benzalkonium bromide antibacterial agent to kill harmful bacteria, but benzalkonium bromide itself, as a fungicide, can kill lactic acid bacteria, and benzalkonium bromide has a certain stimulating effect on the skin , is not conducive to the promotion of the use of

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] An external care lotion containing probiotics, which is prepared by mixed fermentation of Streptococcus salivarius, Lactobacillus acidophilus and Lactobacillus rhamnosus;

[0022] Specifically, the preparation method of the lotion comprises the following steps:

[0023] Prepare culture medium: Add glucose 10g / L, yeast extract 5g / L, sodium acetate 2g / L, potassium dihydrogen phosphate 0.5g / L, magnesium sulfate heptahydrate 0.1g / L, ferrous sulfate heptahydrate 0.1 to deionized water g / L, adjust the pH to 7.0, steam at 121°C for 10 minutes, and naturally cool to room temperature to obtain a culture solution;

[0024] Fermentation: Streptococcus salivarius seed liquid, Lactobacillus acidophilus seed liquid and Lactobacillus rhamnosus seed liquid were mixed according to the volume ratio of 3:2:2 to obtain a mixed seed liquid, and the number of viable bacteria in the mixed seed liquid was controlled to be 10 8 cfu / ml; Inoculate the mixed seed liquid into the culture liquid ac...

Embodiment 2

[0027] An external care lotion containing probiotics, which is prepared by mixed fermentation of Streptococcus salivarius, Lactobacillus acidophilus and Lactobacillus rhamnosus;

[0028] Specifically, the preparation method of the lotion comprises the following steps:

[0029] Prepare culture medium: Add glucose 10g / L, yeast extract 5g / L, sodium acetate 2g / L, potassium dihydrogen phosphate 0.5g / L, magnesium sulfate heptahydrate 0.1g / L, ferrous sulfate heptahydrate 0.1 to deionized water g / L, adjust the pH to 7.0, steam at 121°C for 10 minutes, and naturally cool to room temperature to obtain a culture solution;

[0030] Fermentation: Mix the Streptococcus salivarius seed liquid, Lactobacillus acidophilus seed liquid and Lactobacillus rhamnosus seed liquid according to the volume ratio of 5:3:3 to obtain a mixed seed liquid, and control the number of live bacteria in the mixed seed liquid to 10 9 between cfu / ml; inoculate the mixed seed liquid into the culture liquid according...

Embodiment 3

[0033] Acute Toxicity Test

[0034] 40 rabbits were randomly divided into 4 groups, 10 in each group, half male and half male, and 10% sodium sulfide was used to depilate the back of the rabbits. The diameter of the depilated area was 3cm. The making of damaged skin adopts sterilized iron file to scratch (taking the degree of oozing blood), sprays 0.1, 0.5, 1.0 and 2.0ml of the preparation of the present invention respectively in the depilatory area after depilation, and raises each animal in separate cages, and after 24 hours of administration From 1, 24, 48, 72 hours to the seventh day, observe and record the animal's body weight, fur, luster, diet, eye and mucous membrane changes, respiratory center, nervous system, limb activity and death every day, and record the changes after seven days. Rabbits were sacrificed, and the changes in heart, liver, spleen, lung, kidney and other organs were observed by dissection.

[0035] Results: The lotion prepared by the present inventi...

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PUM

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Abstract

The invention belongs to the technical field of microorganisms, and discloses a probiotics-containing care lotion for external use. The probiotics-containing care lotion for external use is prepared by mixed fermentation of Streptococcus salivarius, Lactobacillus acidophilus and Lactobacillus rhamnosus. The care lotion for external use is a pure microecological preparation, has a good antibacterial effect but has no side effects.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to an external care lotion containing probiotics. Background technique [0002] Vaginitis is a common and frequently-occurring disease in women's reproductive system inflammation. The main symptoms are increased vaginal discharge, itching of the vulva or burning sensation of the genitals. There are many reasons for the occurrence of vaginitis, the main reason is the imbalance of flora. Normal parasitism in the vaginal flora of healthy women, Lactobacillus is dominant, and vaginal Lactobacillus plays a key role in maintaining normal vaginal flora. The glycogen in the vaginal epithelium is decomposed into lactic acid by the action of lactobacilli, making the local vagina a weakly acidic environment. The following conditions may affect the balance of the vaginal ecosystem: ①Sex hormones: For example, estrogen levels decrease before and after the menstrual period, l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/747A61K35/744A61P15/00A61P29/00A61P17/00
CPCA61K35/747A61K9/0014A61K35/744A61P15/00A61P17/00A61P29/00A61K2300/00
Inventor 杨丽华胡艳香郑志翠
Owner 杨丽华
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