A construction method and application of an aptamer-DNA polymer based on nonlinear hybridization chain amplification

A high molecular polymer, hybrid chain reaction technology, applied in the field of biomedical targeted drug delivery system, can solve the problems of low bioavailability, strong side effects, non-targeting, etc., and achieve the effect of increasing application potential
CN109295133BActive Publication Date: 2022-04-19FUZHOU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
FUZHOU UNIV
Publication Date
2022-04-19

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Abstract

The invention discloses the construction and application of a DNA macromolecular polymer based on non-linear hybridization chain amplification. The system introduces an initiating chain into the system, and induces six different non-transforming Clip-like nucleic acid probes cascade self-assembled to form DNA polymers. On this basis, the 5' end of the partially assembled sequence was treated with phosphorylase to form a more stable DNA assembly under the action of ligase. Nucleic acid aptamers are bound to its sticky ends to identify target cells and introduce cytotoxic drug doxorubicin to realize imaging and drug delivery to target cells.
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Description

technical field

[0001] The invention belongs to the technical field of biomedical targeted drug delivery system, and in particular relates to the construction and application of a DNA polymer based on nonlinear hybrid chain amplification. Background technique

[0002] At present, in vitro nucleic acid amplification signal amplification technology to improve detection sensitivity and accuracy has become a hot research direction in the field of molecular biology. Common nucleic acid isothermal signal amplification techniques can be roughly divided into: strand displacement amplification (Strand displacement amplification, SDA), rolling circle amplification (rolling circle amplification, RCA), hybridization chain reaction (Hybridization chain reaction, HCR), Loop-Mediated Isothermal Amplification (LAMP) and so on. HCR technology is a new type of nucleic acid isothermal amplification technology that does not involve enzymes in catalysis and constant temperature. Under certain c...

Claims

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