Thin-layer chromatographic detection method for radix codonopsis and radix astragali stomach-invigorating granules
A technique of thin-layer chromatography and detection method is applied in the field of thin-layer chromatography detection of Shenqi Jianwei granules, which can solve the problem of inability to determine the active ingredients of Shenqi Jianwei granules, etc., and achieve the effect of accurate detection results.
Inactive Publication Date: 2019-02-01
ZHONGXING PHARM CO LTD JIANGSU
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Abstract
The invention discloses a thin-layer chromatographic detection method for radix codonopsis and radix astragali stomach-invigorating granules. The thin-layer chromatographic detection method for radixastragali comprises the following steps of 1) preparing a test solution, taking the radix codonopsis and radix astragali stomach-invigorating granules, performing washing with water-saturated n-butylalcohol, evaporating a combined n-butyl alcohol solution to dryness, adding methanol for dissolving obtained residues, performing arrangement on a neutral alumina column, performing eluting with the methanol, collecting eluant, performing evaporating to dryness, and adding the methanol for dissolving powder, so as to obtain the test solution; 2) preparing a reference solution; 3) applying samplesof the test solution and the reference solution to the same silica gel plate, and performing visual inspection under an ultraviolet lamp; and 4) comparing spots in the corresponding positions of a test color spectrum and a reference color spectrum, thereby indicating that the radix codonopsis and radix astragali stomach-invigorating granules contain the radix astragali component. The method has the advantages that the thin-layer chromatographic method is adopted for carrying out thin-layer chromatographic analysis on the radix astragali component in the radix codonopsis and radix astragali stomach-invigorating granules to detect whether the radix codonopsis and radix astragali stomach-invigorating granules contain the radix astragali component or not, so that the radix astragali componentis effectively detected and a detection result is accurate.
Application Domain
Component separation
Technology Topic
ChemistryThin layer chromatographic +6
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