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Thin-layer chromatographic detection method for radix codonopsis and radix astragali stomach-invigorating granules

A technique of thin-layer chromatography and detection method is applied in the field of thin-layer chromatography detection of Shenqi Jianwei granules, which can solve the problem of inability to determine the active ingredients of Shenqi Jianwei granules, etc., and achieve the effect of accurate detection results.

Inactive Publication Date: 2019-02-01
ZHONGXING PHARM CO LTD JIANGSU
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  • Description
  • Claims
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Problems solved by technology

[0004] Purpose of the invention: In view of the above problems, the purpose of the present invention is to provide a TLC detection method for Shenqi Jianwei Granules, so as to solve the technical problem that the active ingredients in Shenqi Jianwei Granules cannot be judged

Method used

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  • Thin-layer chromatographic detection method for radix codonopsis and radix astragali stomach-invigorating granules
  • Thin-layer chromatographic detection method for radix codonopsis and radix astragali stomach-invigorating granules
  • Thin-layer chromatographic detection method for radix codonopsis and radix astragali stomach-invigorating granules

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Embodiment 1

[0047] A thin-layer chromatographic detection method for Shenqi Jianwei granules, including a thin-layer chromatographic detection method for Radix Astragali, specifically comprising the following steps:

[0048]1) Preparation of the test solution: take Shenqijianwei granules, grind finely, add 50mL water to reflux for 1h, filter, evaporate the filter residue to dryness, add 50mL methanol, reflux for 1h, filter, evaporate the filtrate to dryness, and dissolve the obtained powder in 30mL water , shake and extract twice with water-saturated n-butanol, each time the amount of water-saturated n-butanol is 20mL, combine the n-butanol solution, wash twice with ammonia test solution, each time the amount of ammonia test solution is 20mL, and then Wash twice with water-saturated n-butanol, use 20 mL of water-saturated n-butanol each time, remove the water, evaporate the combined n-butanol solution to dryness, add 5 mL of methanol to dissolve the obtained residue, and put it on neutral ...

Embodiment 2

[0055] A thin-layer chromatographic detection method for Shenqi Jianwei granules, including a thin-layer chromatographic detection method for inulin, specifically comprising the following steps:

[0056] 1) Preparation of the test solution: Take 15 g of Shenqi Jianwei Granules, grind it finely, add 25 mL of dichloromethane, soak in cold for 24 hours, filter, evaporate the filtrate to dry, add 1 mL of dichloromethane to dissolve the residue, and obtain the test solution;

[0057] 2) Preparation of the reference substance mixed solution: take the reference substances of inulin and isocyanolide, add the two into methanol, and prepare the reference substances containing 2 mg / mL inulin and 2 mg / mL of inulin The mixed solution of the reference substance was obtained to obtain the mixed solution of the reference substance;

[0058] 3) Preparation of negative control solution: take a negative sample lacking inulin, and use step 1) to obtain a negative control solution;

[0059] 4) Sp...

Embodiment 3

[0063] A thin-layer chromatography detection method for Shenqi Jianwei granules, including the thin-layer chromatography detection method for Angelica sinensis, specifically comprising the following steps:

[0064] 1) Preparation of the test solution: Take 15 g of Shenqi Jianwei Granules, add ether to extract twice, each time the amount of ether is 20 mL, combine the ether solution, evaporate to dryness under low temperature conditions, add 1 mL of ethyl acetate to dissolve the residue, and obtain the test product solution;

[0065] 2) Preparation of reference drug solution: take 0.25 g of Angelica reference drug and add 2 mL of ether, shake for 10 min, let it stand, and take the supernatant as the reference drug solution;

[0066] 3) Preparation of negative control solution: take the negative sample lacking Angelica sinensis, and use step 1) method to prepare negative control solution;

[0067] 4) Spot each 5 μL of the test solution, control medicinal material solution, and ...

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Abstract

The invention discloses a thin-layer chromatographic detection method for radix codonopsis and radix astragali stomach-invigorating granules. The thin-layer chromatographic detection method for radixastragali comprises the following steps of 1) preparing a test solution, taking the radix codonopsis and radix astragali stomach-invigorating granules, performing washing with water-saturated n-butylalcohol, evaporating a combined n-butyl alcohol solution to dryness, adding methanol for dissolving obtained residues, performing arrangement on a neutral alumina column, performing eluting with the methanol, collecting eluant, performing evaporating to dryness, and adding the methanol for dissolving powder, so as to obtain the test solution; 2) preparing a reference solution; 3) applying samplesof the test solution and the reference solution to the same silica gel plate, and performing visual inspection under an ultraviolet lamp; and 4) comparing spots in the corresponding positions of a test color spectrum and a reference color spectrum, thereby indicating that the radix codonopsis and radix astragali stomach-invigorating granules contain the radix astragali component. The method has the advantages that the thin-layer chromatographic method is adopted for carrying out thin-layer chromatographic analysis on the radix astragali component in the radix codonopsis and radix astragali stomach-invigorating granules to detect whether the radix codonopsis and radix astragali stomach-invigorating granules contain the radix astragali component or not, so that the radix astragali componentis effectively detected and a detection result is accurate.

Description

technical field [0001] The invention relates to the field of thin-layer chromatography detection methods, in particular to a thin-layer chromatography detection method of Shenqi Jianwei granules. Background technique [0002] The prescription of Shenqi Jianwei Granules is derived from the classic prescription "Huangqi Jianzhong Decoction" in "Treatise on Febrile Diseases" by Zhang Zhongjing, a medical sage in the Han Dynasty, and it is compatible with fourteen natural Chinese herbal medicines such as Codonopsis, Astragalus, and Angelica. and stomach. Indications for chronic atrophic gastritis of spleen and stomach deficiency and cold type, suitable for epigastric distension and pain, fullness and discomfort, heat and pressure, belching and hiccups. This variety is included in the tenth volume of traditional Chinese medicine preparations in the "Drug Standards of the Ministry of Health", the standard number WS3-B-1973-95. [0003] However, for the quality standard of Shenqi...

Claims

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Application Information

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IPC IPC(8): G01N30/90
CPCG01N30/90
Inventor 杨琴武俊明
Owner ZHONGXING PHARM CO LTD JIANGSU
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