Microalgae nutrition repair solution for relieving grassland desertification and preparation method thereof

A repair solution and microalgae technology, applied in liquid fertilizers, applications, organic fertilizers, etc., can solve the problems of reducing the degradation of soil microbial transformation of toxic substances, reducing the number and activity of soil microorganisms, and inhibiting the development of plant roots, so as to improve plant growth. , Improve plant stress resistance, prevent plant diseases and insect pests

Pending Publication Date: 2019-02-05
雷云飞
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the soil in desertified areas is poor, and the nutrients that can provide vegetation are seriously lacking, the survival rate of vegetation is low, and the control effect is not good.
The application of chemical fertilizers can improve the survival rate of vegetation to a certain extent, but the negative impact of chemical fertilizers cannot be ignored: the raw materials for the production of chemical fertilizers contain heavy metals such as Zn, Cu, Co, and Cr, and these heavy metals are added to the plant following chemical fertilizers. soil, and cause the enrichment of heavy metal elements in the soil, which enter the plant through plant absorption and affect plant growth; chemical fertilizers will reduce the number and activity of soil microorganisms, thereby reducing the conversion of soil microorganisms to organic matter in the soil, the decomposition of minerals and Degradation of toxic substances; long-term application of chemical fertilizers will aggravate soil acidification, aggravate soil compaction, inhibit plant root development, and seriously reduce plant stress resistance

Method used

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  • Microalgae nutrition repair solution for relieving grassland desertification and preparation method thereof
  • Microalgae nutrition repair solution for relieving grassland desertification and preparation method thereof
  • Microalgae nutrition repair solution for relieving grassland desertification and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A microalgae nutrient restoration solution for alleviating grassland desertification, the preparation method comprising the following steps:

[0041] S1, expanding the cultivation of Monofidobacterium microfidum, Microcoleum, Pseudocladonella, nitrogen-fixing Anabaena and Chlorella pyrenoidosa, respectively, to obtain five kinds of algae liquids, among which, under the expansion of cultivation,

[0042] The expansion cultivation process of the five algae fluids includes: selecting and separating a single microalgae cell under a microscope → culturing a single microalgae cell in 15mL medium solution for 10 days to obtain 15mL algae fluid → transfer to 85mL medium solution Continue culturing for 10 days to obtain 100mL of algae solution → inoculate into 0.9L medium solution for 10 days to obtain 1L of algae solution → inoculate into 4L medium solution for 10 days to obtain 5L Algae liquid → inoculated into 13L medium solution and continued to cultivate for 10 days to obta...

Embodiment 2

[0047] The difference from Example 1 is that in the expanded cultivation of step S1,

[0048] The expansion cultivation process of the five algae fluids includes: selecting and separating a single microalgae cell under a microscope → culturing a single microalgae cell in 15mL medium solution for 12 days to obtain 15mL algae fluid → transfer to 85mL medium solution Continue culturing for 10 days to obtain 100mL of algae liquid → inoculate into 0.9L medium solution for 12 days to obtain 1L of algae liquid → inoculate into 4L medium solution for 12 days to obtain 5L Algae liquid → inoculated into 13L medium solution and continued to cultivate for 12 days to obtain 18L of algae liquid → transferred to 782L medium solution and continued to cultivate for 12 days to obtain 800L of algae liquid. At the end of the expansion of each stage above, the number of viable microalgae cells is ≥10 6 cells / mL, the number of active microalgae cells in the final obtained algae liquid is ≥10 6 a / ...

Embodiment 3

[0051] The difference from Example 1 is that in the expanded cultivation of step S1,

[0052] The expansion cultivation process of the five algae liquids includes: selecting and separating a single microalgal cell under a microscope → culturing a single microalgal cell in 15mL of medium solution for 15 days to obtain 15mL of algae liquid → transfer to 85mL of medium solution Continue culturing for 15 days, obtain 100mL of algae liquid → inoculate into 0.9L medium solution and continue culturing for 15 days, obtain 1L of algae liquid → transfer and inoculate into 4L medium solution for 15 days, obtain 5L Algae liquid → inoculated into 13L medium solution and continued to cultivate for 15 days to obtain 18L of algae liquid → transferred to 782L medium solution and continued to cultivate for 15 days to obtain 800L of algae liquid. At the end of the expansion of each stage above, the number of viable microalgae cells is ≥10 6 cells / mL, the number of active microalgae cells in the...

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Abstract

The invention discloses a microalgae nutrition repair solution for relieving grassland desertification, and belongs to the technical field of desertification control. The microalgae nutrition repair solution for relieving the grassland desertification is characterized in that multiple types of microalgae in a living body state are contained. Calculated by a number of microalgae cell individuals, the microalgae comprise 5-40 parts of Tolypothrix tenuis, 10-50 parts of Microcoleus sp., 5-40 parts of Scytonema sp., 10-50 parts of Anabaena azotica, and 30-80 parts of Chlorella pyrenoidosa. The invention further discloses a preparation method of the microalgae nutrition repair solution for relieving the grassland desertification. The preparation method comprises the following steps: S1, respectively amplifying breeding of the Tolypothrix tenuis, the Microcoleus sp., the Scytonema sp., the Anabaena azotica and the Chlorella pyrenoidosa, so as to acquire five types of algae fluid; and S2, mixing the five types of algae fluid. The microalgae nutrition repair solution for relieving the grassland desertification has the effects of repairing soil, replenishing natural nitrogen, activating soil microelements, improving plant adverse resistance, and promoting plant growth in allusion to a grassland desertification area.

Description

technical field [0001] The invention relates to the technical field of desertification prevention and control, in particular to a microalgae nutrient restoration solution for alleviating grassland desertification and a preparation method thereof. Background technique [0002] At present, two-thirds of the countries and regions in the world, nearly 35.3×10 8 m 2 The land area of ​​China is facing the threat of desertification, which has seriously affected the living space and sustainable development of human beings, making desertification an ecological and environmental issue of global concern. In desertified areas, the land is degraded, productivity declines, surface wind erosion is desertified, and sand dunes move and bury farmland, roads, and houses. The ecology is fragile and the economic conditions are relatively backward, so it is very difficult to prevent and control. [0003] Improving the vegetation environment is an important means of combating desertification, su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F11/08C05G3/04C05G1/00C05G3/80
CPCC05B7/00C05F11/08C05G3/80C05G5/20C05C5/02C05D3/00C05D5/00C05D9/00C05D9/02C05F11/00
Inventor 雷云飞
Owner 雷云飞
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