High-density culture method for yeast
A high-density culture, yeast technology, applied in fungi and other directions, can solve the problems of complex control equipment, not very suitable, and achieve the effects of low investment, improved utilization, and simple operation
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[0033] Example 1
[0034] 1) Batch culture: Fill a 5L mechanically agitated ventilated fermentor with 1L of culture medium, connect with 100mL of yeast seed culture liquid to start fermentation, and the concentration of yeast seed liquid is 1×10 8 Pcs / ml, dilute H is used in the fermentation process 2 SO 4 Or the dilute NaOH solution maintains the pH between 4.5-5.0, and the jacket is passed through cooling water to control the fermentation temperature at 26-28°C.
[0035] 2) Exponential feeding: the fermentable sugar is consumed in batch cultivation for about 18 hours, and the feeding pump is turned on when the dissolved oxygen reaches 60%. The feeding method is according to the feeding formula Execute (where the yeast specific growth rate μ is set to 0.02h -1 ).
[0036] 3) Dissolved oxygen feedback pulse flow feed: exponential flow is fed for about 45 hours, 2.0g / L ethanol is detected in the fermentation broth, and the feeding method is changed to dissolved oxygen feedback pulse f...
Example Embodiment
[0037] Example 2
[0038] 1) Batch cultivation: Fill a 5L mechanically agitated ventilated fermentor with 1L of culture medium, connect with 100mL of seed culture solution to start fermentation, and the concentration of yeast seed solution is 1×10 8 Pcs / ml, dilute H is used in the fermentation process 2 SO 4 Or the dilute NaOH solution maintains the pH between 5.0-5.5, and the jacket is passed through cooling water to control the fermentation temperature at 28-30°C.
[0039] 2) Exponential feeding: the fermentable sugar is consumed in batch cultivation for about 18 hours, and the feeding pump is turned on when the dissolved oxygen reaches 60%. The feeding method is according to the feeding formula Execute (where the yeast specific growth rate μ is set to 0.03h -1 ).
[0040] 3) Dissolved oxygen feedback pulse flow feed: exponential flow is fed for about 45 hours, 2.0g / L ethanol is detected in the fermentation broth, and the feeding method is changed to dissolved oxygen feedback pulse...
Example Embodiment
[0041] Example 3
[0042] 1) Batch cultivation: Fill a 5L mechanically agitated ventilated fermentor with 1L of culture medium, connect with 100mL of seed culture solution to start fermentation, and the concentration of yeast seed solution is 1×10 8 Pcs / ml, dilute H is used in the fermentation process 2 SO 4 Or the dilute NaOH solution maintains the pH between 5.5-6.5, and the jacket passes cooling water to control the fermentation temperature at 30-34°C.
[0043] 2) Exponential feeding: the fermentable sugar is consumed in batch cultivation for about 18 hours, and the feeding pump is turned on when the dissolved oxygen reaches 60%. The feeding method is according to the feeding formula Implementation (where the specific growth rate of yeast μ is set to 0.04h -1 ).
[0044] 3) Dissolved oxygen feedback pulse flow feed: exponential flow is fed for about 45 hours, 2.0g / L ethanol is detected in the fermentation broth, and the feeding method is changed to dissolved oxygen feedback pulse ...
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