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High-density culture method for yeast

A high-density culture, yeast technology, applied in fungi and other directions, can solve the problems of complex control equipment, not very suitable, and achieve the effects of low investment, improved utilization, and simple operation

Active Publication Date: 2019-02-12
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods are not very suitable for most small and medium-sized yeast production enterprises, because more advanced methods often involve complex control equipment and implement complex control algorithms, and require necessary auxiliary equipment. These additional and expensive inputs is a serious obstacle in the path of modernization for most SMEs

Method used

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Examples

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Effect test

Embodiment 1

[0034] 1) Batch culture: put 1L medium in a 5L mechanically stirred ventilated fermenter, insert 100mL yeast seed culture liquid to start fermentation, and the yeast seed liquid cell concentration is 1×10 8 unit / ml, dilute H was used in the fermentation process 2 SO 4 Or dilute NaOH solution to maintain the pH between 4.5-5.0, and the jacket passes through cooling water to control the fermentation temperature at 26-28°C.

[0035] 2) Exponential fed-batch feeding: After about 18 hours of batch culture, the fermentable sugar is consumed, and when the dissolved oxygen rises to 60%, the feeding pump is turned on, and the feeding method is according to the feeding formula Execute (wherein the yeast specific growth rate μ is set to 0.02h -1 ).

[0036] 3) Dissolved oxygen feedback pulse feeding: Exponential feeding for about 45 hours, 2.0g / L ethanol was detected in the fermentation broth, and the feeding mode was changed to dissolved oxygen feedback pulse feeding, that is, dissol...

Embodiment 2

[0038] 1) Batch culture: put 1L medium in a 5L mechanically stirred ventilated fermenter, insert 100mL seed culture solution to start fermentation, and the concentration of yeast seed solution is 1×10 8 unit / ml, dilute H was used in the fermentation process 2 SO 4 Or dilute NaOH solution to maintain the pH between 5.0-5.5, and the jacket passes cooling water to control the fermentation temperature at 28-30°C.

[0039] 2) Exponential fed-batch feeding: After about 18 hours of batch culture, the fermentable sugar is consumed, and when the dissolved oxygen rises to 60%, the feeding pump is turned on, and the feeding method is according to the feeding formula Execute (the specific growth rate μ of yeast is set to 0.03h -1 ).

[0040] 3) Dissolved oxygen feedback pulse feeding: Exponential feeding for about 45 hours, 2.0g / L ethanol was detected in the fermentation broth, and the feeding mode was changed to dissolved oxygen feedback pulse feeding, that is, dissolved oxygen was a...

Embodiment 3

[0042] 1) Batch culture: put 1L medium in a 5L mechanically stirred ventilated fermenter, insert 100mL seed culture solution to start fermentation, and the concentration of yeast seed solution is 1×10 8 unit / ml, dilute H was used in the fermentation process 2 SO 4 Or dilute NaOH solution to maintain the pH between 5.5-6.5, and the jacket passes cooling water to control the fermentation temperature to 30-34°C.

[0043] 2) Exponential fed-batch feeding: After about 18 hours of batch culture, the fermentable sugar is consumed, and when the dissolved oxygen rises to 60%, the feeding pump is turned on, and the feeding method is according to the feeding formula Execution (wherein the yeast specific growth rate μ is set to 0.04h -1 ).

[0044] 3) Dissolved oxygen feedback pulse feeding: Exponential feeding for about 45 hours, 2.0g / L ethanol was detected in the fermentation broth, and the feeding mode was changed to dissolved oxygen feedback pulse feeding, that is, dissolved oxyge...

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Abstract

The invention discloses a high-density culture method for a yeast, and belongs to the field of yeast fermentation. Through a two-stage feeding mode combining index feeding and dissolved oxygen feedback pulse fed-batch feeding, the yield of a by-product ethyl alcohol is effectively controlled to be at a low level, the utilization ratio of a molasses raw material is improved, meanwhile, the yeast can be ensured to be quickly proliferated to high concentration, the dry cell weight of yeast cells can reach to 123.6g.L<-1>, the yield of yeast mycelium is improved, 0.51g of dry strain can be obtained at most per gram of sugar, and the productivity of small and medium-sized enterprises is favorably improved on the basis of the original scale.

Description

technical field [0001] The invention relates to a method for high-density cultivation of yeast, which belongs to the field of yeast fermentation. Background technique [0002] Yeast and its by-products can be widely used in food, medicine, feed and other industries, and have a broad application market and good economic benefits. In 2013, the global market for yeast products reached US$5.8 billion, and it is expected to reach US$9.2 billion in 2019. Molasses is a by-product of sugar processing. It contains 17-25% water, about 50% sugar, and some nitrogenous substances, vitamins and trace elements. It is the main raw material for cultivating yeast. [0003] The glucose effect that occurs during yeast culture is a factor that restricts the growth of yeast. The so-called glucose effect means that when the concentration of fermentable carbon source is too high, even if the oxygen supply is sufficient, the carbon source will flow to the ethanol synthesis pathway, resulting in Th...

Claims

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Application Information

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IPC IPC(8): C12N1/16
CPCC12N1/16
Inventor 王宇徐富增张建华王柯陈旭升张宏建
Owner JIANGNAN UNIV
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