Preparation method of epigallocatechin gallate cross-linked small intestinal submucosa guided bone regeneration membrane

A technology of epigallocatechin and small intestinal submucosa, applied in tissue regeneration, medical science, surgery, etc., can solve problems such as poor space maintenance ability, fast degradation speed, poor mechanical properties of bone regeneration membrane, etc., to overcome mechanical properties Poor, slow degradation, good biocompatibility effects

Active Publication Date: 2019-02-15
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It overcomes the shortcomings of the existing small intestinal submucosa guiding bone regeneration me

Method used

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  • Preparation method of epigallocatechin gallate cross-linked small intestinal submucosa guided bone regeneration membrane
  • Preparation method of epigallocatechin gallate cross-linked small intestinal submucosa guided bone regeneration membrane
  • Preparation method of epigallocatechin gallate cross-linked small intestinal submucosa guided bone regeneration membrane

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1 A kind of preparation method of EGCG cross-linked SIS guided bone regeneration membrane

[0043] 1. Preparation method of SIS film:

[0044] (1) Cleaning and cutting: take a pair of fresh pig small intestines that were slaughtered within 4 hours, and only take the jejunum section, wash it repeatedly with a large amount of water, cut it longitudinally along the mesentery and cut it into 15cm-20cm intestine sections;

[0045] (2) Mechanical curettage: mechanically scrape off the serosal layer and muscular layer outside the cut intestinal segment, rinse it in normal saline for 3 times, each time for 5 minutes;

[0046] (3) Degreasing: Wring out the rinsed film, put it into 1000mL (methanol: chloroform volume ratio is 1:1) degreasing solution, put it in a fume hood for 12 hours, and replace the degreasing solution once in the middle;

[0047] (4) Rinse: Pour off the degreasing solution, rinse the degreasing film repeatedly with a large amount of flowing water ...

Embodiment 2

[0059] Example 2 A preparation method of EGCG cross-linked SIS-guided bone regeneration membrane

[0060] 1. Preparation method of SIS film:

[0061] Same as Example 1.

[0062] 2. EGCG cross-linked SIS film:

[0063] 1) Preparation of cross-linking solution: Weigh 0.25 g of EGCG powder and dissolve it in 100 mL of PBS solution to prepare a cross-linking solution with a concentration of 0.25%, and store in the dark;

[0064] 2) Cross-linking of SIS membrane: Put a piece of SIS membrane (150mg) into 25mL of the prepared cross-linking solution, and cross-link in the dark at 37°C for 48 hours;

[0065] 3) Cleaning of the cross-linked SIS membrane: repeatedly washing the cross-linked SIS membrane with PBS and deionized water; lyophilization and sterilization:

[0066] a. Single-layer material: lay the cross-linked SIS film flat, with the mucous membrane facing up, freeze-dry in a vacuum freeze dryer, and sterilize with ethylene oxide;

[0067] b. Double-layer material: Lay the...

Embodiment 3

[0069] Example 3 A preparation method of EGCG cross-linked SIS-guided bone regeneration membrane

[0070] 1. Preparation method of SIS film:

[0071] Same as Example 1.

[0072] 2. EGCG cross-linked SIS film:

[0073] 1) Preparation of cross-linking solution: Weigh 0.5 g of EGCG powder and dissolve it in 100 mL of PBS solution, prepare a cross-linking solution with a concentration of 0.5%, and store in the dark;

[0074] 2) Cross-linking of SIS membrane: Put a piece of SIS membrane (150mg) into 25mL of the prepared cross-linking solution, and cross-link in the dark at 37°C for 48 hours;

[0075] 3) Cleaning of the cross-linked SIS membrane: repeatedly washing the cross-linked SIS membrane with PBS and deionized water; lyophilization and sterilization:

[0076] a. Single-layer material: lay the cross-linked SIS film flat, with the mucous membrane facing up, freeze-dry in a vacuum freeze dryer, and sterilize with ethylene oxide;

[0077] b. Double-layer material: Lay the cro...

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Abstract

The invention discloses a material with a bone repair effect. The material is epigallocatechin gallate cross-linked small intestinal submucosa, and the cross-linking index of the submucosa is 15.76-21.44%. The invention further provides a preparation method and application of the material. The material disclosed by the invention has excellent mechanical property and slow degradation, is capable ofmaintaining a space structure at the bone tissue regeneration position, has excellent biocompatibility and bone formation effects, overcomes the defects that the conventional small intestinal submucosa membrane serving as a guided bone regeneration membrane is poor in mechanical property, high in degradation speed, poor in space maintenance ability and the like, and has excellent clinical application prospects.

Description

technical field [0001] The invention relates to a preparation method of an epigallocatechin gallate cross-linked small intestinal submucosa guided bone regeneration membrane. Background technique [0002] Implant restoration has a high success rate and will not damage adjacent teeth, so it has become the first choice for the restoration of single missing teeth. For patients with multiple tooth loss and dentition loss, implant restoration also has a high success rate. Compared with traditional denture restoration, implant restoration has better stability, comfort and aesthetics, improves chewing efficiency, and greatly improves the quality of life of patients. The achievement and long-term maintenance of successful osseointegration is one of the most important prerequisites for successful implant restorations, namely the presence of sufficient healthy bone mass at the implant site. This does not only refer to sufficient bone height to accommodate implants of the appropriate...

Claims

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Application Information

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IPC IPC(8): A61L31/00A61L31/14
CPCA61L31/00A61L31/005A61L31/14A61L31/148A61L2300/412A61L2430/40A61L2430/02
Inventor 解慧琪苟敏王航江燕林
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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