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Method for determining pesticide and chemical residues in fruits and vegetables

A determination method and chemical technology, which can be used in measurement devices, scientific instruments, instruments, etc., can solve the problems of poor mixing and extraction effect, high sample weight, low efficiency, etc., to reduce measurement costs, improve efficiency, reduce The effect of the concentration process

Inactive Publication Date: 2019-02-15
成都市华测检测技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, the present invention proposes a method for the determination of pesticide and chemical residues in fruits and vegetables, which solves the problems of poor mixing and extraction effect, too long time-consuming and low efficiency caused by the high sample weight of the existing measurement method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A method for measuring pesticide and chemical residues in fruits and vegetables, comprising the following steps:

[0034] S1, grind grape skins into juice, accurately weigh 10g of grape juice and place in a centrifuge tube;

[0035] S2, accurately add 40mL of acetonitrile to the centrifuge tube and mix evenly with the grape juice to form a mixture A;

[0036] S3, ultrasonically extracting the mixture A for 30 minutes;

[0037] S4, adding 5 g of sodium chloride to the mixture A after ultrasonic extraction and mixing to form a mixture B;

[0038] S5, put the mixture B into a centrifuge after vortexing for 1 min, and centrifuge at 3000r / min for 5 min to separate the layers to obtain the supernatant A and the lower sediment A;

[0039] S6, extracting the layered supernatant A;

[0040] S7, adding 40mL of acetonitrile to the precipitate A to form a mixture C;

[0041] S8, the mixture C was subjected to ultrasonic extraction for 30 minutes and centrifuged for layering, an...

Embodiment 2

[0051] A method for measuring pesticide and chemical residues in fruits and vegetables, comprising the following steps:

[0052] S1, grind the capsicum with the skin on, accurately weigh 10g of the capsicum and place it in a centrifuge tube;

[0053] S2, accurately add 40mL of acetonitrile in the centrifuge tube and evenly mix with pepper to form mixture A;

[0054] S3, ultrasonically extracting the mixture A for 30 minutes;

[0055] S4, adding 6.5 mL of acetone to the mixture A after ultrasonic extraction and mixing to form a mixture B;

[0056] S5, put the mixture B into a centrifuge after vortexing for 1 min, and centrifuge at 3000r / min for 5 min to separate the layers to obtain the supernatant A and the lower sediment A;

[0057] S6, extracting the layered supernatant A;

[0058] S7, adding 40mL of acetonitrile to the precipitate A to form a mixture C;

[0059] S8, the mixture C was subjected to ultrasonic extraction for 30 minutes and centrifuged for layering, and the...

Embodiment 3

[0069] A method for measuring pesticide and chemical residues in fruits and vegetables, comprising the following steps:

[0070] S1, grind the eggplant with its skin into juice, accurately weigh 10g of eggplant juice and place it in a centrifuge tube;

[0071] S2, accurately add 40mL of extracting solution to the centrifuge tube and uniformly mix with grape juice to form mixture A, the extracting solution is composed of the following components by volume percentage: 25% sodium oxyethylene ether sulfate, 7% lauryl diethanolamine, acetone 15%, n-hexane 30%, adenosine triphosphate disodium 13%, acetonitrile 10%;

[0072] S3, ultrasonically extracting the mixture A for 30 minutes;

[0073] S4, add 6.5mL acetone to the mixture A after ultrasonic extraction and mix to form mixture B; strengthen the demulsification effect;

[0074] S5, put the mixture B into a centrifuge after vortexing for 1 min, and centrifuge at 3000r / min for 5 min to separate the layers to obtain the supernatan...

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Abstract

The invention provides a method for determining the residual amount of pesticides and chemicals in fruits and vegetables, comprising the following steps: weigh the sample in a centrifuge tube; add acetonitrile; ultrasonic extraction after a period of 30 minutes; add 5 grams of sodium chloride; undergo centrifugation and stratification; extract supernatant liquor A; add acetonitrile; start ultrasonic extraction and centrifugation; extract supernatant liquor B, and mix it with supernatant liquor A to obtain supernatant liquor C; concentrate supernatant C to be purified; pass supernatant C through a C / N column; elute the C / N column; collect the elution solution, and heat the concentrated solution; add n-hexane; add the internal standard solution; mix the mixture. The invention has the beneficial effects that the mixing and extracting effect in the centrifuge tube is good, the absorption process is shortened, the concentration process is reduced, the time consumption is shortened, and theefficiency is improved.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to a method for determining pesticide and chemical residues in fruits and vegetables. Background technique [0002] According to the provisions of "GB / T 19648-2006 Determination of 500 Pesticides and Related Chemical Residues in Fruits and Vegetables by Gas Chromatography-Mass Spectrometry", the determination method for pesticides and chemical residues in fruits and vegetables is: extraction : 20g sample in a centrifuge tube → add 40mL acetonitrile → 15000r / min homogenate for 1min, → add 5g sodium chloride → homogenate for 1min, centrifuge → take 20mL supernatant (equivalent to 10g sample) → to be purified; : Pass the purified solution through the Envi-18 column → collect the filtrate and concentrate it to about 1mL at 40°C → pass through the pre-activated Envi-Carb and Sep-Pak tandem column, acetonitrile-toluene solution (3+1) to elute the tandem column, collect Concentrate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 贾健松熊子灵黄娟
Owner 成都市华测检测技术有限公司