Preparation method for extracting small molecule peptide from maize
A technology of small molecular peptides and corn, which is applied in the field of preparation of small molecular peptides extracted from corn, can solve the problems of low nutritional value and poor taste of corn peptides, and achieve high nutritional value, high content of small molecular peptides, and good taste Effect
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[0020] The present invention provides a kind of preparation method of corn small molecule peptide, comprises the following steps:
[0021] 1) Grinding corn, mixing the obtained corn flour with water, heating at 100-110°C and a pressure of 0.05-0.2MPa for 170-190min to obtain a wall-breaking solution;
[0022] 2) After the wall-breaking liquid in the step 1) is cooled to 50-60°C, add a biological compound enzyme for enzymolysis, and inactivate the obtained enzymolysis liquid to obtain an inactivation liquid;
[0023] The biological complex enzyme is glutenase and cellulase;
[0024] 3) Centrifuging and membrane-separating the inactivation solution in step 2) in sequence to obtain corn polypeptide.
[0025] The invention grinds the corn, mixes the obtained corn powder with water, and heats for 170-190 minutes under the conditions of 100-110° C. and pressure of 0.05-0.2 MPa to obtain the wall-breaking liquid. In the present invention, the crushed particle size is preferably 24-...
Embodiment 1
[0030] 1) Weigh 1000g of corn, grind the corn into 40 mesh corn flour, mix the corn flour with 10kg of water, heat at 110°C and pressure of 0.05MPa for 190min, and obtain the wall breaking solution;
[0031] 2) Cool the wall-breaking solution obtained in step 1) to 60°C, add 3g of formulated biological compound enzyme (the mass ratio of glutenase and cellulase is 1:1, the enzymatic activity of glutenase is 4000IU, cellulose The enzyme activity of the enzyme is 10000IU), and the enzymolysis is carried out at 55°C to obtain the enzymolysis solution;
[0032] 3) subjecting the enzymolysis solution obtained in step 2) to inactivation treatment at 100° C. for 30 minutes to obtain an inactivation solution;
[0033] 4) Centrifuge the inactivation solution at 3400rpm, separate the supernatant with a membrane with a pore size of 100nm, collect the permeate after membrane separation, and pass the permeate through a low-temperature falling membrane concentration method (60°C, -0.006Mpa ...
Embodiment 2
[0035] 1) Weigh 1000g of corn, grind the corn into 40 mesh corn flour, mix the corn flour with 11kg of water, heat at 105°C and pressure of 0.1MPa for 180min, and obtain the wall breaking solution;
[0036] 2) Cool the wall-breaking solution obtained in step 1) to 50°C, add 4.5g of formulated biological compound enzyme (the mass ratio of glutenase and cellulase is 1:0.9, the enzymatic activity of glutenase is 10000IU, fiber Sulfase activity is 10000IU) carries out catalytic enzymolysis, obtains enzymolysis solution;
[0037] 3) subjecting the enzymolysis solution obtained in step 2) to inactivation treatment at 100° C. for 15 minutes to obtain an inactivation solution;
[0038] 4) Centrifuge the inactivation solution at 3500rpm, separate the supernatant with a membrane with a pore size of 100nm, collect the permeate, and concentrate the permeate through the membrane concentration method (GE brand membrane for purified water). The liquid was dried by spray method (175°C) to ob...
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