Method for extracting kaempferol galactose compound from camellia semiserrata Chi

A technology for galactose and kaempferol, which is applied in the field of extracting kaempferol galactose compounds, can solve problems such as insufficient research, and achieve the effects of simple extraction method and high extraction purity

Active Publication Date: 2019-02-22
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, not enough research has been done on the specific components in the extract

Method used

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  • Method for extracting kaempferol galactose compound from camellia semiserrata Chi
  • Method for extracting kaempferol galactose compound from camellia semiserrata Chi
  • Method for extracting kaempferol galactose compound from camellia semiserrata Chi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The tea fruit of Nanshan tea is dried, shelled, and the cake produced after removing the oil by physical pressing method is dried and properly crushed to obtain Nanshan tea cake. Ultrasonic leaching was performed with 50% ethanol aqueous solution at 60°C for 3 times, each 2h, with a solid-liquid ratio of 1:4 (m / v), and then filtered and combined to obtain an extract. Then, the extract was concentrated and extracted with an equal volume of n-butanol to obtain an extract, which was then diluted 20 times with water to obtain a sample solution.

[0051] The purpose of shelling and degreasing is to maximize the extraction yield and reduce the interference in the separation process. Appropriate feedstock handling methods also help to avoid loss of target compounds.

[0052] The model of the macroporous resin is selected as D101 type, first soak the D101 macroporous resin with ethanol, and then pack the column (column volume BV 1 10L), rinse with ethanol and water in turn un...

Embodiment 2

[0058] The tea fruit of Nanshan tea is dried, shelled, and the cake produced after removing the oil by physical pressing method is dried and properly crushed to obtain Nanshan tea cake. Ultrasonic leaching was performed twice with 60% ethanol aqueous solution at 70°C for 2 hours each time, with a solid-liquid ratio of 1:2 (m / v), and then filtered and combined to obtain an extract. Then, the extract was concentrated, extracted with an equal volume of n-butanol to obtain the extract, and then diluted 22 times with water to obtain the sample solution.

[0059] The model of the macroporous resin is selected as D101 type, first soak the D101 macroporous resin with ethanol, and then pack the column (column volume BV 1 10L), rinse with ethanol and water in turn until there is no ethanol smell, can be detected with an alcohol meter, and the reading is less than 5. Slowly add the sample solution to the macroporous resin chromatography column, and then use 6 times BV in turn. 1 The vo...

Embodiment 3

[0064] The tea fruit of Nanshan tea is dried, shelled, and the cake produced after removing the oil by physical pressing method is dried and properly crushed to obtain Nanshan tea cake. Ultrasonic leaching was carried out with 55% ethanol aqueous solution at 65°C for 4 times, each time for 2 hours, and the material-liquid ratio was 1:4 (m / v), and then filtered and combined to obtain the extract. Then, the extract was concentrated and extracted with an equal volume of n-butanol to obtain an extract, which was then diluted 18 times with water to obtain a sample solution.

[0065] The model of the macroporous resin is selected as D101 type, first soak the D101 macroporous resin with ethanol, and then pack the column (column volume BV 1 10L), rinse with ethanol and water in turn until there is no ethanol smell, can be detected with an alcohol meter, and the reading is less than 5. Slowly add the sample solution to the macroporous resin chromatography column, and then use 6 times ...

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Abstract

The invention provides a method for extracting a kaempferol galactose compound from camellia semiserrata Chi. The method includes the steps that a camellia semiserrata Chi cake is subjected to leaching, concentration, extraction and dilution to obtain a sample solution; the sample solution is loaded with a wet method, gradient elution is carried out with an ethanol aqueous solution system, and eluent containing the target compound is collected and then subjected to aftertreatment to obtain a crude component solution; the crude component solution is loaded with a wet method to a polyamide resinchromatographic column for gradient elution, and eluent is collected and then concentrated and freeze-dried to obtain a crude extract; the crude extract is dissolved by a solvent and then subjected to DAC preparation, and crude isolate is obtained; the crude isolate is dissolved by a solvent and then subjected to DAC preparation, and pre-isolate is obtained; the pre-isolate is dissolved by a solvent and then subjected to sample injection so as to be subjected to isocratic elution, and the target compound is obtained. By means of the method, the target compound is effectively separated from camellia plants for the first time, and the extraction purity is high.

Description

technical field [0001] The present invention relates to the field of compound extraction, in particular to a method for extracting kaempferol galactose compounds from Camellia sinensis. Background technique [0002] Nanshan tea, also known as Guangning safflower oil tea (Camellia semiserrata Chi.), belongs to the Camellia L. (Camellia L.) plant of the family Theaceae, and is a perennial tree or shrub. Nanshan tea is mainly distributed in southeastern Guangxi and western Guangdong in China. It is an important cultivated species of Camellia oleifera. After research and comparison, it was found that among all kinds of Camellia oleifera, the chemical components in Guangning safflower Camellia are relatively rich. [0003] Nanshan tea and its cakes are especially rich in compounds such as flavonoids and saponins with various biological activities. At present, the research on Camellia oleifera meal mainly focuses on the optimization of various extraction processes based on tota...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08
CPCC07H1/08C07H17/07
Inventor 曹清明袁欢易英建张智昆方艺
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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