Composite microbial agent and application thereof in tobacco stems

A technology of compound microbial bacterial agent and bacterial agent, which is applied in the field of patent application matters to achieve the effect of improving the quality of tobacco stems, low processing cost, and good promotion and application value

Inactive Publication Date: 2019-02-22
CHINA TOBACCO HENAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Theoretically speaking, the use of microbial strains to ferment tobacco raw materials can achieve technical effects similar to natural aging treatment, that is, on the one hand, it can reduce the content of harmful components, and on the other hand, it can improve the quality of tobacco raw materials. Screening and combined application of bacterial species and microbial strains. Therefore, different combinations of target microbial strains and microbial strains need to be verified in order to obtain better application guarantees

Method used

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  • Composite microbial agent and application thereof in tobacco stems
  • Composite microbial agent and application thereof in tobacco stems
  • Composite microbial agent and application thereof in tobacco stems

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The composite microbial bacterial agent provided in this application is specifically prepared through the following steps.

[0044] (1) Preparation of Bacillus subtilis XC-19-1 active bacterial agent

[0045] Place the Bacillus subtilis XC-19-1 strain on the LB plate, activate and cultivate it at 37°C for 72 hours, collect the cultured colonies, inoculate them in liquid LB medium, and cultivate them overnight on a shaker at 37°C as the seed solution;

[0046] The seed solution was transferred to liquid LB medium at a volume ratio of 1%, and cultivated to OD at 30°C and 150 rpm. 600 =2.0 or so as fermentation broth;

[0047] Centrifuge the fermentation broth at 3600 rpm for 10min, discard the supernatant, then add sterile water to fully suspend, and adjust the number of viable bacteria to 11×10 7 A / mL spare.

[0048] (2) Preparation of Bacillus pumilus SMXP-3 active bacterial agent

[0049] Put the Bacillus pumilus SMXP-3 strain on the LB plate, and after 72 hours of...

Embodiment 2

[0059] When using the composite microbial agent prepared in Example 1 to treat tobacco stems, in order to determine the optimal ratio of the composite microbial agent and its specific effect on tobacco stems, the inventor carried out partial orthogonal experiment optimization. The specific process is as follows: :

[0060] According to the single dosage ratio of each active bacterial agent in the tobacco stem, the three kinds of bacterial solutions were absorbed and mixed. After adjusting the number of viable bacteria, the mixed bacterial solution was evenly applied to the cut stems (100 g of cut stems for each treatment), and at the same time Set up a blank control group and three kinds of single-bacteria liquid treatment groups, then adjust the moisture content of cut stems in each experimental group to 25%, place them in a constant temperature and humidity chamber (control temperature at 37°C, and humidity at 75%), and take them out after 96 hours of fermentation , placed i...

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Abstract

The invention belongs to the technical field of production and processing of cigarette raw materials and in particular relates to a composite microbial agent prepared by utilizing a specific microbialstrain and patent application matters of application thereof in tobacco stems. The active ingredients of the agent comprise bacillus subtilis XC-19-1, bacillus pumilus strains SMXP-3 and living bacteria of bacillus pumilus SMXP-58, and the agent is a complex obtained by mixing liquid culture of various bacteria. By virtue of screening of specific strains and complex development of the composite microbial agent, a simple technical scheme of treating the tobacco stems by the composite microbial agent is designed. Therefore, the composite microbial agent has the technical advantages that the process flow is simple and convenient, the treatment cost is low, the treatment cycle is short, the content of cellulose, proteins and pectin in the tobacco stems can be obviously reduced, and the quality of the tobacco stems is improved. Therefore, the composite microbial agent has excellent popularization and application value in the technical field of cigarette manufacturing.

Description

technical field [0001] The invention belongs to the technical field of production and processing of cigarette raw materials, and in particular relates to a patent application for a composite microbial agent prepared by using a specific microbial strain and its application in tobacco stems. Background technique [0002] Tobacco stems are the thick and hard veins of tobacco leaves, accounting for about 25% of tobacco leaves, and are the main raw materials for making shredded stems that play a supporting role in cigarettes. In the early days of cigarette production, it was generally believed that tobacco stems had more impurities and poor smokeability after burning, so they were only used in the production of low-grade cigarettes. However, with the update of cigarette preparation technology and health awareness, in the production of existing cigarettes, it is generally believed that the application of appropriate content of cut stems can not only improve the filling performance...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A24B5/16A24B15/20C12R1/125C12R1/07
CPCA24B5/16A24B15/20C12N1/20
Inventor 杨宗灿冯颖杰程良琨刘超张展张婷婷许衡李家美彭玉富申洪涛陈伟
Owner CHINA TOBACCO HENAN IND
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