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Serum urea measuring reagent, and applications thereof

A reagent, urea technology, applied in the field of serum urea determination reagents, can solve the problems of poor stability of blank absorbance change rate, etc., achieve the effect of improving stability, reasonable reagent formula, and improving reliability

Inactive Publication Date: 2019-02-26
AILEX TECH GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the deficiencies of the prior art and meet the needs of the market, the present invention provides a serum urea assay reagent and its application. By optimizing the reagent formula and adjusting the pH of the buffer solution, the problem of poor stability of the blank absorbance change rate is solved. Provides the reliability of hospital clinical test results, has broad prospects and huge market value

Method used

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  • Serum urea measuring reagent, and applications thereof
  • Serum urea measuring reagent, and applications thereof
  • Serum urea measuring reagent, and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1 preparation reagent

[0042] The components of reagent R1 are: Capso, NADH, EDTA.Na 2 , NaN 3

[0043] The components of reagent R2 are: HEPES, ɑ-ketoglutarate, urease, glutamate dehydrogenase, EDTA.Na 2 , Thioglycolic acid, BSA, NaN 3 ;

[0044] Add the above components into purified water, stir, mix and dissolve to obtain the reagent.

Embodiment 2

[0045] Example 2 Assembly kit

[0046] The reagents R1 and R2 prepared in Example 1, instructions, etc. were assembled into a kit.

Embodiment 3

[0047] Embodiment 3 detection method

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Abstract

The invention provides a serum urea measuring reagent, and applications thereof. The serum urea measuring reagent comprises a reagent R1 and a reagent R2; the reagent R1 comprises following raw materials: Capso, NADH, and EDTA.Na2; and the reagent R2 comprises following raw materials: HEPES, alpha-ketoglutaric acid, urase, glutamate dehydrogenase, EDTA.Na2, a reducing agent, and BSA. Reagent formula is optimized, and buffer pH is adjusted, so that effective buffering capacity is provided, NADH stability is improved, tool enzyme oxidation is reduced, component synergistic interaction and effectimprovement are achieved, a problem in the prior art that blank absorbance change rate stability is poor is solved, hospital clinical detection results are reliable, the application prospect is promising, and the market value is extremely large.

Description

technical field [0001] The invention belongs to the field of biology, and relates to a reagent for measuring serum urea and an application thereof. Background technique [0002] Urea is the end product of protein metabolism in the body. It has a small molecular weight and does not bind to plasma proteins. It can be freely filtered by the glomerulus. When the human kidney parenchyma is damaged, the glomerular filtration rate decreases and the urea in the blood increases. By measuring the urea content in the blood, the glomerular filtration function can be observed. [0003] At present, the commonly used method for detecting serum urea in hospitals is the enzyme coupling rate method. The determination principle is: urea is hydrolyzed into NH4+ and carbon dioxide under the action of urease, NH4+, reducing coenzyme Ⅰ (NADH) and α-ketoglutarate Glutamic acid is generated under the action of amino acid dehydrogenase, and NADH is oxidized to NAD+ at the same time, and the absorban...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N35/00G01N21/31
CPCG01N21/31G01N35/00
Inventor 李子樵程贝郑丽媛翁飞凤
Owner AILEX TECH GRP CO LTD
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