Screening model of drugs against head and neck cancer tumors

A tumor drug, head and neck technology, used in compound screening/testing, tumor/cancer cells, microorganisms, etc., can solve the problems of insufficient clinical applicability, complex model construction, and excessive time, and shorten the time required for drug efficacy experiments. The time required, the growth rate is fast, and the success rate is high.

Pending Publication Date: 2019-03-01
苏州致诺优生物医学有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Aiming at the problems of complex construction, long time, high cost and insufficient clinical applicability of the existing anti-head and neck cancer tumor screening drug models, the present invention provides a screening model for anti-head and neck cancer tumor drugs

Method used

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  • Screening model of drugs against head and neck cancer tumors
  • Screening model of drugs against head and neck cancer tumors
  • Screening model of drugs against head and neck cancer tumors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Establishment of Pathological Model of Mouse Head and Neck Cancer Tumor

[0031] 1. Preparation of hollow fiber reactor

[0032] refer to figure 1 , the hollow fiber reactor includes a plurality of hollow fibers 1 arranged in parallel, and a plurality of holes 2 are distributed on the surface of each hollow fiber tube. The material of the hollow fiber filaments used in the hollow fiber reactor is polypropylene or polychlorene, and the hollow fiber The diameter of the tube is 70-100 μm, the wall thickness is 2-4 μm, the length is 3-5 cm, the diameter of the hole is 100-300 nm, and 140 hollow fiber reactors are used in the experiment, and the surface of the hollow fiber reactor is modified with acetic acid plasma 10 minutes, and sterilized with UV light for 30 minutes to ensure that the cells are in a sterile state.

[0033] 2. Establishment of head and neck cancer tumor model in mice

[0034] 2.1 Establishment of general head and neck cancer tumor cell mode...

Embodiment 2

[0038] Example 2: Establishment of a screening model for anti-head and neck cancer drugs

[0039] 1. Experimental grouping

[0040] The 10 mice of the general head and neck cancer tumor model were divided into a SAS blank group and a SAS administration group, with 5 mice in each group. The mice in the MCF blank group were not given anticancer drugs, and the mice in the SAS administration group were given Doxorubicin (DOX) at a dose of Doxorubicin 20 mg / kg.

[0041] The 25 mice of the head and neck cancer tumor model were divided into control group, cis group, Carbo group, Doce group and 5-Fu group, with 5 mice in each group. The cis group was given Cisplatin, and the Carbo group was given carboplatin. , the Doce group was given Docetaxel, and the 5-Fu group was given 5-Fu. The dosage was Cisplatin 20mg / kg, carboplatin 20mg / kg, Docetaxel 20mg / kg, and 5-Fu 20mg / kg.

[0042] 2. Screening model for anti-head and neck cancer tumor drugs

[0043] In 10 mice of the general head an...

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Abstract

The invention discloses a screening model of drugs against head and neck cancer tumors. The screening model is constructed through the specific steps that (1) the surface of a hollow fiber reactor istransformed through acetic acid plasma, and sterilization treatment is conducted; (2) head and neck cancer tumor single cells are separated; (3) the head and neck cancer tumor single cells are plantedinto the hollow fiber reactor for cell adhesion culture; (4) a target drug is determined, a healthy mouse serves as a drug delivery target, the hollow fiber reactor is taken and planted into the backof the mouse, and a mouse head and neck cancer tumor pathological model is established after 24 h; and (5) the target drug is applied to the mouse head and neck cancer tumor pathological model, the drug is delivered once every two days, and the screening model of the drugs against the head and neck cancer tumors is constructed. According to the screening model, efficacy evaluation of the drugs against the head and neck cancer tumors can be quickly conducted, the time required by a pharmacological experiment of a traditional mouse xenograft model is greatly shortened, the time required from the experiment to the result does not exceed 20 days, the clinical experiment cost can be lowered, and the screening model is easy to popularize.

Description

technical field [0001] The invention relates to the field of drug efficacy screening, in particular to a screening model for anti-head and neck cancer tumor drugs. Background technique [0002] Head and neck tumors include neck tumors, ENT tumors, and oral and maxillofacial tumors. Neck tumors belong to general surgery in general hospitals, and the more common ones are thyroid tumors; common otolaryngology tumors include laryngeal cancer, paranasal sinus cancer, etc.; oral and maxillofacial tumors are common oral cancers, such as tongue cancer, Gum cancer, buccal cancer, etc. Therefore, the tumors that occur in the head and neck have many primary sites and pathological types, ranking first in systemic tumors. However, the median survival time of patients with head and neck tumor recurrence or metastasis is only about 6 months. Patients who fail first-line therapy experience a sharp decline in survival, usually dying within 3 months. Chemotherapy is currently the main tre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/13A61K49/00C12N5/09A01K67/027
CPCA61K35/13A01K67/0271A01K2207/12A01K2227/105A01K2267/0331A61K49/0008C12N5/0693C12N2509/00
Inventor 赖俊凯陈仲奇陈献堂赵美玲
Owner 苏州致诺优生物医学有限公司
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